Clean production method for extracting tabersonine from African machilus fruits

Abstract

The invention provides a clean production method for extracting tabersonine from African machilus fruits. The method comprises the following steps: (1) reflux extraction: crushing an African machilusfruit raw material, putting the crushed African machilus fruit raw material into an extraction tank, adding a mixed solvent of a lipophilic organic solvent containing a hydrophobic betaine derivativeand a medium-polarity organic solvent, carrying out heating reflux, and filtering to obtain a mixed solvent extracting solution; and (2) silica gel chromatographic column adsorption: enabling the mixed solvent extracting solution to pass through a silica gel chromatographic column, collecting silica gel chromatographic column effluent, and concentrating until no solvent exists so as to obtain a brownish yellow oily matter, namely the voacanga chaenomeles speciosa volatile oil; and (3) silica gel chromatographic column elution: eluting the silica gel chromatographic column with an eluent, collecting the eluent, and concentrating the eluent under reduced pressure, cooling the concentrated solution, stirring for crystallization, carrying out suction filtration, and drying to obtain the tabersonine finished product. According to the method, inorganic acid, alkali and water are not used, no sewage or waste gas is discharged, and the tabersonine finished product is high in content, high in yield, environmentally friendly and suitable for industrial production.

Description

technical field [0001] The invention relates to a method for separating active components of African potato fruit, in particular to a clean production method for extracting tabonin from African potato fruit. Background technique [0002] African potato fruit (Voacanga Africana), also known as African Voacanga tree, is a perennial tree of the Apocynaceae family. It is mainly distributed in Africa from Senegal to Sudan and Angola, as well as in West Africa, Congo and Tanzania. African potato fruit has a long history of medicinal use in traditional medicine in Africa. The whole plant can be used as medicine and is often used to treat diseases such as leprosy, dysentery, malaria, generalized edema, epilepsy and convulsions in children. Pharmacological activity studies have shown that African potato fruit has anti-tumor, anti-bacterial, anti-inflammatory, anti-plasmodium, anti-angiogenesis, memory-improving, sedative-hypnotic and detoxification activities, and has extremely high ...

AI Technical Summary

Problems solved by technology

This method requires expensive equipment such as ultrafine grinding and ultrasonic extraction, and the steps are cumbersome. There may be a large amount of TritonX-114 residues in the product, which is not suitable for industrial production.

In addition, this method uses concentrated hydrochloric acid and water, and there will inevitably be waste water discharge, which cannot be called a real "clean process"

In addition, the detection method of this patent is not the external standard method, but the normalization method

There are still many flaws in the normalization method to test the purity. Moisture, inorganic salts, and impurity components that cannot be displayed under liquid phase conditions cannot be reflected by the normalization method.

Therefore, the product obtained in this patented method has a purity of 99%, and the actual purity still has flaws.