Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus rhamnosus lm1019 strain, and composition for preventing and treating obesity or diabetes including same

A technology of Lactobacillus rhamnosus and a composition, applied in the field of a composition for preventing and treating obesity or diabetes, the Lactobacillus rhamnosus LM1019 strain field, can solve the problems of undisclosed lipolysis inhibiting effect and appetite regulating effect and the like

Active Publication Date: 2020-10-20
GENOME & CO +1
View PDF16 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] In this regard, Japanese Patent Application No. 2016520305 (published on July 14, 2016) discloses the WIKIM31 strain of Lactobacillus genus with anti-obesity activity, and in U.S. Patent Application No. 2015 / 0283186 (published on October 8, 2015 Publication) discloses Lactobacillus rhamnosus CNCM I-3690 strain that is effective in managing obesity, and Korean Patent No. 101611837 (published on April 06, 2016) discloses a method for preventing or treating obesity and metabolic diseases caused by obesity Lactobacillus rhamnosus CBT LR5 strain, but the lipolysis inhibitory effect and appetite regulating effect of these strains in small intestinal cells or digestive tract are not disclosed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus rhamnosus lm1019 strain, and composition for preventing and treating obesity or diabetes including same
  • Lactobacillus rhamnosus lm1019 strain, and composition for preventing and treating obesity or diabetes including same
  • Lactobacillus rhamnosus lm1019 strain, and composition for preventing and treating obesity or diabetes including same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Adipocyte differentiation inhibition and fat accumulation inhibition in 3T3-L1 preadipocytes

[0061] In order to confirm the adipocyte differentiation inhibitory effect and the intracellular fat accumulation inhibitory effect in preadipocytes of the Lactobacillus rhamnosus LM1019 strain deposited with the Korean Collection of Microorganisms under the accession number KCCM12308P, the following experiments were performed.

[0062] First, differentiated adipocyte staining was accomplished using Oil Red O on 3T3-L1 preadipocytes treated with 1% LM1019 culture extract, 10% LM1019 culture extract, and phosphate buffered saline as a control, 1 The stained cells were observed by microscopy after 1 hour.

[0063] like figure 1 As shown, as a result of comparison with the control group to which phosphate buffered saline was added instead of the LM1019 culture extract, it was confirmed that the number of fat globules (arrows) in the adipocytes of the treated group treated with t...

Embodiment 2

[0067] Neutral lipolysis promotion and accumulation inhibition in 3T3-L1 adipocytes

[0068] In order to confirm the amount of intracellular neutral fat (TG, triglyceride) accumulated in the process of differentiation into adipocytes, 1% and 10% of the extract of the LM1019 strain were added and cultured, respectively. As a control group, phosphoric acid was used. Buffered saline solution was used instead of strain extract for cultivation. Adipocytes from day 9 of differentiation were obtained and used.

[0069] The obtained cells were washed three times with a phosphate buffered saline solution and the cells were disrupted, and then centrifuged at 15,000 rpm under a temperature condition of 4°C, and the supernatant was used as a sample. 10 μL of the sample and 150 μL of an enzyme reagent were added, and the reaction was carried out under normal temperature conditions for 15 minutes to measure the absorbance at 530 nm.

[0070] The results of the measurement of neutral fat i...

Embodiment 3

[0076] Determination of lipolytic enzyme (lipase) activity

[0077] In order to confirm the lipolytic enzyme (lipase) activity of the LM1019 strain, the following experiments were performed.

[0078] By preparing a lipase production medium, LM1019 was streaking in a loop, and incubated at a temperature of 37°C overnight. When lipase is produced, olive oil (olive oil) in the medium is decomposed to produce monoglyceride (monoglyceride).

[0079] Monoglyceride emits light in ultraviolet (UV) at 350 nm by binding to Rhodamine B (Rhodamin B) in the medium, and a comparison experiment was performed with a control group using Escherichia coli (E.coli), the control group and the LM1019 strain group Neither glowed.

[0080] The concentration of monoglyceride in the control group was 0.75 nmol / well, and the concentration in the LM1019 strain group was 0.70 nmol / well. Compared with the control group, there was no difference in the concentration of monoglyceride in the strain group.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a Lactobacillus rhamnosus LM1019 strain (accession number: KCCM12308P), and a composition for preventing and treating obesity or diabetes including same. Specifically,the Lactobacillus rhamnosus LM1019 strain according to the present invention reduces fat cells, neutral lipids, subcutaneous fat, and cholesterol, thus having an obesity prevention and treatment effect, and also reduces the blood concentrations of glucose and insulin, thereby suppressing insulin resistance and thus having a diabetes prevention and treatment effect.

Description

technical field [0001] The present invention relates to a novel Lactobacillus rhamnosus LM1019 strain and a composition comprising the same for preventing and treating obesity or diabetes. Specifically, the Lactobacillus rhamnosus LM1019 strain of the present invention has the effect of preventing and treating obesity by reducing adipocytes, neutral fat, subcutaneous fat and cholesterol, and at the same time suppressing blood sugar concentration and insulin concentration by reducing Insulin resistance to prevent and treat diabetes. In addition, Lactobacillus rhamnosus LM1019 is also involved in the expression of lipase as a lipolytic enzyme, leptin as an appetite regulating hormone, and the obesity-related gene carnitine palmitoyltransferase (CPT). Background technique [0002] Recently, due to the improvement of income level and the development of industries, the diet and eating habits have been rapidly westernized, and the number of patients with chronic diseases and adul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12A23K10/16A23L29/00A23L33/135A61K35/747C12R1/225
CPCA23C9/1203A23L33/135A23V2002/00A23V2200/328A23V2200/332A61K35/747C12R2001/225C12N1/205A61P3/04A61P3/10A23K10/18A23V2400/175A23K10/16A23L29/065C12N1/20A23L33/40A61K9/0056A61K2035/115
Inventor 朴汉洙
Owner GENOME & CO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com