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Anti-biofilm antibiotic hydrogel as well as preparation method and application thereof

A biofilm and antibiotic technology, applied in biochemical equipment and methods, botanical equipment and methods, chemicals for biological control, etc. The effect of simple preparation method and short action time

Active Publication Date: 2020-10-13
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this type of gel can reasonably administer aminoglycoside antibiotics to a certain extent, the killing effect on biofilm bacteria is severely reduced, and the minimum inhibitory concentration on film bacteria is several hundred times that of free bacteria.

Method used

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  • Anti-biofilm antibiotic hydrogel as well as preparation method and application thereof
  • Anti-biofilm antibiotic hydrogel as well as preparation method and application thereof
  • Anti-biofilm antibiotic hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: Effect of pectinase on the sensitivity of aminoglycoside antibiotics to kill free bacteria and film-coated bacteria

[0049] Amikacin was selected as the model representative drug of aminoglycoside antibiotics, and pectinase was selected as the representative of the polysaccharide degradation component in the anti-biofilm preparation. Pseudomonas aeruginosa was inoculated in a 96-well plate, 100 μL per well, and the initial concentration of bacteria was 10 4 CFU / mL, placed in an incubator at 37°C and incubated for three days, during which the medium was replaced once a day to cultivate a mature biofilm. On the fourth day, the medium was removed, fresh medium was added, and different concentrations of amikacin were added to detect its minimum inhibitory concentration on bacteria in the mature biofilm. At the same time, a certain concentration of pectinase (200u / mL) was introduced into different concentrations of amikacin solutions, and the minimum inhibito...

Embodiment 2

[0051] Embodiment 2: the impact of neutral protease on the sensitivity of aminoglycoside antibiotics to kill free bacteria and film-coated bacteria

[0052] Prepare mature biofilm according to the method in Example 1, and take the same method to add neutral protease to process mature biofilm, such as figure 2 As shown, it can be observed that when the amount of neutral protease used is 3u / mL, the killing performance of amikacin on the filmed bacteria can be significantly improved, and the MIC value of 640ug / mL is reduced to 8ug / mL.

Embodiment 3

[0053] Embodiment 3: Effect of phosphatase on the sensitivity of aminoglycoside antibiotics to kill free bacteria and film-coated bacteria

[0054] Prepare mature biofilm according to the method in embodiment 1, and take the same method to add phosphatase to process mature biofilm, such as image 3 As shown, it can be observed that when the phosphatase is 12.5u / mL, the killing performance of amikacin on the filmed bacteria can be significantly improved, and the MIC value of 640ug / mL is reduced to 16ug / mL.

[0055] 2. Preparation of aminoglycoside / anti-biofilm agent / oxidized polysaccharide polymer composite gel:

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Abstract

The invention provides an antibiotic hydrogel capable of degrading a biofilm. The antibiotic hydrogel is formed by crosslinking oxidized polysaccharide macromolecules, biological enzymes capable of degrading the biofilm and aminoglycoside antibiotics through acid-sensitive Schiff base bonds. The invention further provides antibacterial application of the antibiotic hydrogel capable of degrading the biofilm in vitro and in vivo. According to the antibiotic hydrogel capable of degrading the biofilm provided by the invention, on one hand, the aminoglycoside antibiotics can be released as required; and on the other hand, a biological enzyme component for degrading biofilm matrix is released at the same time, the biofilm is destroyed, the sensitivity of aminoglycoside antibiotics for treating biofilm infection is improved, and the biofilm is expected to be applied to various clinical anti-infection treatments.

Description

technical field [0001] The invention belongs to the field of biological materials and antibacterial, and relates to an antibiotic hydrogel for antibacterial biofilm, a preparation method and application thereof. Background technique [0002] In recent years, the abuse and irrational use of antibiotics have led to toxic side effects and drug resistance. For example, when the drug dose is lower than the minimum effective drug concentration, it can increase bacterial virulence and promote bacterial drug resistance by inducing gene mutations; high Drug concentration will produce deafness, kidney damage and other toxic side effects. And bacteria usually exist in the form of biofilm, which is composed of exopolysaccharides, extracellular proteins secreted by bacteria, dead bacteria and nucleic acids produced by lysis, and becomes a barrier that hinders the penetration and diffusion of antibiotics and other drugs. And to help bacteria escape the immune capture of organisms, the dr...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/36A61K38/47A61K38/48A61K38/46A61P31/04A01N63/50A01N43/16A01N25/04A01P1/00A61K31/7036A61K31/7048A61K31/702
CPCA61K9/06A61K47/36A61K38/47A61K38/48A61K38/465A61K31/7036A61K31/7048A61K31/702A61P31/04C12Y302/02015C12Y304/00C12Y301/03A01N63/00A01N43/16A01N25/04A61K2300/00
Inventor 程义云胡婧婧胡倩瑜
Owner EAST CHINA NORMAL UNIV
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