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Water-soluble conjugated polymer with antibacterial and antiviral functions as well as preparation and application thereof

A technology of conjugated polymers and water-soluble polythiophene, which is applied in the direction of antiviral agents, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve economic losses and social burdens, bacterial drug-resistant viruses Antimicrobial resistance and other problems, to achieve good antibacterial, long-term use effect

Active Publication Date: 2020-10-02
河北凯尔威生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The drug resistance of bacteria and viruses has become a difficult problem in clinical treatment, causing huge economic losses and social burdens

Method used

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  • Water-soluble conjugated polymer with antibacterial and antiviral functions as well as preparation and application thereof
  • Water-soluble conjugated polymer with antibacterial and antiviral functions as well as preparation and application thereof
  • Water-soluble conjugated polymer with antibacterial and antiviral functions as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the synthesis of water-soluble conjugated polymer

[0054] Add 10mL chloroform, anhydrous ferric chloride (325mg, 2mmol), and monomer M120.2mg (0.1mmol), monomer M3 224.2mg (0.8mmol), monomer M4 59.3mg (0.1mmol) into the reaction flask in sequence ), so that the total substance amount was 1 mmol, and reacted at room temperature for 24 hours. The reaction was quenched with methanol and centrifuged, washed with methanol / chloroform (1:1), and tetrabutylammonium chloride was added to form a black precipitate. The precipitate was filtered and dissolved in methanol, spin-dried, washed, and dialyzed for 2 days with a dialysis bag with a molecular weight cut-off of 3500 Da; the solvent was removed under reduced pressure and vacuum-dried to obtain a purple-black solid.

Embodiment 2

[0055] Embodiment 2, water-soluble conjugated polymer is used for killing escherichia coli

[0056] 100 μL of a solution of the water-soluble conjugated polymer (prepared in Example 1) was prepared with ultrapure water to make the concentration reach 30 μg / mL. Add 100 μL of Escherichia coli suspension with OD600=1. The system was left standing at 4°C for 5 minutes, and the bacteria and the water-soluble conjugated polymer were mixed evenly by shaking. Then put it in a 37°C incubator and incubate it for ten minutes, and illuminate it with 0.5W white light for 5 minutes, then take 10 μL of the bacterial solution and dilute it 104 times, take 20 μL to smear the plate, and count the number of colonies after culturing in a 37°C incubator for 12 hours. . Among them, the solution without water-soluble conjugated polymer and the experimental group added with water-soluble conjugated polymer but without light were used as controls. The result is attached figure 1 As shown, the resu...

Embodiment 3

[0057] Embodiment 3, water-soluble polythiophene complex is used for killing EV71 virus

[0058] The water-soluble conjugated polymer (the polymer prepared in Example 1) was formulated into a solution so that the concentration of the water-soluble conjugated polymer was 1 mM, and put into 1.5mL centrifuge tubes respectively, according to the sample amount in the following table Prepare the solution

[0059]

[0060] Then add 0.2mL EV71 virus venom and mix well. The experimental group Tn was illuminated at room temperature for 30 minutes, and the light source intensity was not less than 40mW / cm2 (50W halogen tungsten lamp, light distance 30cm), and the control group C was placed in a dark box at room temperature for 30 minutes. The sample that takes is carried out the mensuration of viral half infectious dose (TCID50), and its processing result is as attached figure 2 shown. According to the results of the accompanying drawings, it can be observed that the material has a...

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Abstract

The invention provides a water-soluble conjugated polymer with antibacterial and antiviral functions as well as preparation and application thereof. Water-soluble polythiophene is used as a main component, and a monomer forming the water-soluble polythiophene and other acceptor units are copolymerized to form the water-soluble polythiophene, wherein the monomer forming the water-soluble polythiophene at least includes the monomer represented as the following structural formula; and the structural formula of the water-soluble conjugated polymer is shown as a formula I. Compared with the traditional antibacterial and antiviral drugs, the antibacterial and antiviral water-soluble conjugated polymer obtained by the invention has the characteristics of high efficiency, long service life and thelike. The conjugated polymer provided by the invention has good antibacterial and antiviral effects under an illumination condition, and meanwhile, the conjugated polymer provided by the invention isantibacterial and not easy to generate drug resistance, and the development period is shorter than that of antibacterial and antiviral drugs, so that the conjugated polymer can be efficiently appliedto clinical antibacterial and antiviral treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a water-soluble conjugated polymer with antibacterial and antiviral functions and its preparation and application, more specifically to a photofunctional polymer that can avoid drug resistance of bacteria and viruses and Its preparation method and application. Background technique [0002] The drug resistance of bacteria and viruses has become a difficult problem in clinical treatment, causing huge economic losses and social burdens. Due to the abuse of antibiotics and the strong variability of the virus itself, new treatments that can effectively avoid bacterial and viral drug resistance have become research hotspots in the fields of medicine and biology. [0003] The method of photodynamic therapy and photothermal therapy is a very effective treatment method for drug resistance of bacteria or viruses. This method generates biomolecular toxic substances (such as reactive...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G61/12A61K31/795A61K41/00A61K47/59A61P31/04A61P31/10A61P31/14A61P31/16
CPCA61K31/795A61K41/0052A61K41/0057A61K47/59A61P31/04A61P31/10A61P31/14A61P31/16C08G61/126C08G2261/1424C08G2261/143C08G2261/18C08G2261/3223C08G2261/3241C08G2261/43
Inventor 展永邢成芬高冬齐俊杰安海龙
Owner 河北凯尔威生物技术有限公司
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