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Method for detecting GPC3 based on light addressing potential sensor

A technology of sensitive unit and solution, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of high cost, cumbersome operation and complexity, and achieve the effect of reducing detection limit and improving specificity

Active Publication Date: 2020-09-22
GUILIN UNIV OF ELECTRONIC TECH
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  • Application Information

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However, the method is cumbersome, complicated and expensive

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  • Method for detecting GPC3 based on light addressing potential sensor
  • Method for detecting GPC3 based on light addressing potential sensor
  • Method for detecting GPC3 based on light addressing potential sensor

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Embodiment Construction

[0037] The present invention will be described in detail below in combination with specific embodiments.

[0038] figure 1 is a schematic diagram of detecting GPC3 based on a photo-addressable potentiometric sensor. AuNPs / PEI-rGO nanocomposites were synthesized by a simple mixing method. The working surface of the LAPS chip was washed with NaOH solution, the LAPS chip was modified with aminosilylating reagent (APTES), and the modified LAPS chip was activated with carbodiimide / N-hydroxysuccinimide (EDC / NHS). AuNPs / PEI-rGO solution and GPC3-Apt solution were added dropwise on the surface to form the LAPS chip sensitive unit; then the standard solution of GPC3 protein was dropped on the LAPS chip sensitive unit to form the LAPS test substrate. Finally, put the LAPS test substrate into the LAPS test system. Under the action of an external bias voltage, the specific binding of GPC3 protein to GPC3-Apt will cause the potential change on the surface of the sensitive unit, and the (I-...

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Abstract

The invention discloses a method for detecting GPC3 based on a light addressing potential sensor. An LAPS chip, a light source driving circuit, a signal amplification circuit and a LabVIEW platform form an LAPS real-time test system. An AuNPs / PEI-rGO composite material is designed and synthesized; the AuNPs / PEI-rGO and the GPC3-Apt are modified on an LAPS chip to form a sensitive unit. A GPC3 standard solution is dropwise added on the sensitive unit to form a test substrate of the LAPS; under the action of external bias voltage, the specific binding of GPC3 and GPC3-Apt on the LAPS test substrate causes the change of the potential of the surface of the sensitive unit, and the I-V curve generates corresponding offset; the offset and the concentration of the GPC3 show a good linear relationat 0.1-100 [mu] g / mL, and detection of the GPC3 is realized.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting GPC3 based on an optical addressing potential sensor. Background technique [0002] Glypican-3 (GPC3) is phosphatidylinositol proteoglycan 3. The main detection methods include fluorescent immunoassay, enzyme-linked immunosorbent assay (ELISA), and electrochemical analysis methods. Immunological methods for measuring serum GPC3 have poor detection sensitivity and are difficult to apply clinically. The invention patent with the publication number CN 106645724A discloses a detection method for the surface marker molecule GPC3 of circulating tumor cells. By lysing red blood cells in the blood, all the remaining nucleated cells are tiled and enriched on the nano-substrate and fixed by using nanotechnology. All cells were labeled with nuclear fluorescent dye DAPI, all cells were incubated with GPC3 primary antibody, and then incubated w...

Claims

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Application Information

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IPC IPC(8): G01N27/00G01N23/2251G01N33/68
CPCG01N27/00G01N23/2251G01N33/68
Inventor 李桂银周治德赵乐陈敏
Owner GUILIN UNIV OF ELECTRONIC TECH
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