Injectable hydrogel for promoting bone regeneration and preparation method of injectable hydrogel
A technology for injecting water and hydrogel, which is applied in the field of biomedical engineering materials, can solve the problems of non-existing and limiting osteoinductivity, and achieve the effects of promoting osteogenic differentiation, good biocompatibility, and promoting in situ bone regeneration
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Embodiment 1
[0033] The preparation of embodiment 1 chitosan methacrylate (CSMA)
[0034] Weigh 3g of chitosan and dissolve it in 100mL of 2% acetic acid to form a chitosan solution. After ultrasonication for 2min to fully remove air bubbles, slowly add 0.3g of methacrylic anhydride into the chitosan solution dropwise within 5min. , when the two are completely mixed and there are no obvious bubbles, stir and react at room temperature for 12 hours, then transfer to a dialysis bag with a molecular weight cut-off of 3500Da, and dialyze thoroughly with distilled water and deionized water successively, and obtain a light yellow product after vacuum freeze-drying , Dry and fluffy water-soluble chitosan methacrylate.
Embodiment 2
[0035] Example 2 Preparation of CS-PAM-Mg hydrogel
[0036] The chitosan methacrylate of 1.5g, the pamidronate of 100mM and the magnesium chloride of 100mM are dissolved in the PBS solution, with 800r / min stirring reaction 3min, because PAM and Mg 2+ Driven by the effective coordination among them, they serve as cross-linkers to stabilize the aggregated network of CS-PAM, enabling the formation of CS-PAM-Mg nanocomposite hydrogels.
Embodiment 3
[0037] Example 3 Preparation of CS-PAM-Mg / BMP-2 hydrogel
[0038] An injectable hydrogel for promoting bone regeneration, said hydrogel comprising the following components in mass percentage: 95% chitosan methacrylate, 2% pamidronate, 1% osteoinductive factor BMP-2 , magnesium chloride 2%, the solution of the hydrogel is PBS buffer solution. Wherein, the preparation method of chitosan methacrylate is the same as in Example 1.
[0039] The preparation method of the injectable hydrogel that promotes bone regeneration in this embodiment is:
[0040] (1) Chitosan methacrylate, pamidronate, osteoinductive factor BMP-2, and magnesium chloride were dissolved in PBS buffer solution respectively to form chitosan methacrylate solution, pamidronate solution, bone Inducible factor BMP-2 solution, magnesium chloride solution, the concentration of methacrylic acid chitosan solution is 350mM, the concentration of pamidronate solution is 100mM, the concentration of osteoinductive factor BMP...
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