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Injectable hydrogel for promoting bone regeneration and preparation method of injectable hydrogel

A technology for injecting water and hydrogel, which is applied in the field of biomedical engineering materials, can solve the problems of non-existing and limiting osteoinductivity, and achieve the effects of promoting osteogenic differentiation, good biocompatibility, and promoting in situ bone regeneration

Inactive Publication Date: 2020-09-15
GUANGZHOU CHUANGSAI BIOLOGICAL MEDICAL MATERIALS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, commonly used scaffolds are usually composed of bioactive glass, calcium phosphates (such as hydroxyapatite and β-tricalcium phosphate), and biopolymers, which have no or limited osteoinductive properties.

Method used

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  • Injectable hydrogel for promoting bone regeneration and preparation method of injectable hydrogel
  • Injectable hydrogel for promoting bone regeneration and preparation method of injectable hydrogel
  • Injectable hydrogel for promoting bone regeneration and preparation method of injectable hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 chitosan methacrylate (CSMA)

[0034] Weigh 3g of chitosan and dissolve it in 100mL of 2% acetic acid to form a chitosan solution. After ultrasonication for 2min to fully remove air bubbles, slowly add 0.3g of methacrylic anhydride into the chitosan solution dropwise within 5min. , when the two are completely mixed and there are no obvious bubbles, stir and react at room temperature for 12 hours, then transfer to a dialysis bag with a molecular weight cut-off of 3500Da, and dialyze thoroughly with distilled water and deionized water successively, and obtain a light yellow product after vacuum freeze-drying , Dry and fluffy water-soluble chitosan methacrylate.

Embodiment 2

[0035] Example 2 Preparation of CS-PAM-Mg hydrogel

[0036] The chitosan methacrylate of 1.5g, the pamidronate of 100mM and the magnesium chloride of 100mM are dissolved in the PBS solution, with 800r / min stirring reaction 3min, because PAM and Mg 2+ Driven by the effective coordination among them, they serve as cross-linkers to stabilize the aggregated network of CS-PAM, enabling the formation of CS-PAM-Mg nanocomposite hydrogels.

Embodiment 3

[0037] Example 3 Preparation of CS-PAM-Mg / BMP-2 hydrogel

[0038] An injectable hydrogel for promoting bone regeneration, said hydrogel comprising the following components in mass percentage: 95% chitosan methacrylate, 2% pamidronate, 1% osteoinductive factor BMP-2 , magnesium chloride 2%, the solution of the hydrogel is PBS buffer solution. Wherein, the preparation method of chitosan methacrylate is the same as in Example 1.

[0039] The preparation method of the injectable hydrogel that promotes bone regeneration in this embodiment is:

[0040] (1) Chitosan methacrylate, pamidronate, osteoinductive factor BMP-2, and magnesium chloride were dissolved in PBS buffer solution respectively to form chitosan methacrylate solution, pamidronate solution, bone Inducible factor BMP-2 solution, magnesium chloride solution, the concentration of methacrylic acid chitosan solution is 350mM, the concentration of pamidronate solution is 100mM, the concentration of osteoinductive factor BMP...

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Abstract

The invention relates to injectable hydrogel for promoting bone regeneration and a preparation method of the injectable hydrogel, and belongs to the technical field of biomedical engineering materials. The invention provides a bioactive nano-composite hydrogel based on chitosan methacrylate and pami magnesium phosphate nano-particles. The hydrogel is used for local delivery and on-demand simultaneous release of bioactive ions and biological factors. The nano-composite hydrogel prepared by the invention has good injectability and effective stress relaxation, so that the hydrogel is easy to inject and adapts to bone defects with irregular shapes. Magnesium ions released by the hydrogel promote osteogenic differentiation of human mesenchymal stem cells (hMSCs) and activation of alkaline phosphatase (ALP). In addition, a bone induction factor BMP-2 is released to further promote osteogenic differentiation of the hMSC. Results show that the injectable nano-composite hydrogel mediates optimized release of various treatment factors, and effectively promotes in-situ bone regeneration through minimally invasive surgery.

Description

technical field [0001] The invention relates to an injectable hydrogel for promoting bone regeneration and a preparation method thereof, belonging to the technical field of biomedical engineering materials. Background technique [0002] The delivery and long-term administration of therapeutic drugs such as protein-based growth factors, small molecule drugs, genes, and bioactive ions are crucial for the effective treatment of many pathological diseases. It is still challenging to deliver these different drugs with different properties simultaneously with the same carrier . Hydrogels possess a unique three-dimensional cross-linked polymer network comprising a wide range of chemical compositions and physical properties, and are widely used in drug delivery applications. However, due to the inherent permeability and limited network interactions of carrier molecules in hydrogels, sustained delivery is usually achieved through macromolecules such as protein-like growth factors, w...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/02A61L27/50A61L27/52A61L27/54C08B37/08
CPCA61L27/025A61L27/20A61L27/227A61L27/50A61L27/52A61L27/54A61L2300/102A61L2300/216A61L2300/236A61L2300/252A61L2300/412A61L2300/602A61L2400/06A61L2430/02C08B37/003C08L5/08C08L89/00
Inventor 郭瑞文荣蓝咏冯龙宝
Owner GUANGZHOU CHUANGSAI BIOLOGICAL MEDICAL MATERIALS CO LTD
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