A kind of preparation method of magnetically immobilized lipase

Abstract

The invention discloses a preparation method for magnetic immobilized lipase. The preparation method comprises the following steps of hydrolyzing a mixture of tetraethoxysilane and methyl triethoxysilane to obtain transparent sol by taking dilute hydrochloric acid as a catalyst at a normal temperature, wherein the mass ratio of tetraethoxysilane to methyl triethoxysilane in the mixture is (1 to 2)-(5 to 1); after cooling the transparent sol to the room temperature, adding water while performing stirring, and then extracting and removing alcohol as a byproduct produced in a reaction system; andadding magnetic nano Fe3O4 particles in the sol obtained after treatment in the step S2, adjusting a pH value of the system to be 5-7 by using a dilute alkali solution under stirring, adding a lipasesolution, performing stirring to form gel, performing coarse crushing on the gel, placing the crushed gel in an oven of 20-40 DEG C for drying for 1-6h, finely grinding the dried gel, sieving the ground gel, and performing bagging for standby. By adopting the preparation method, for a finally obtained product of immobilized lipase, the activity can reach 80 percent or above of the activity of free lipase, and the immobilized lipase keeps stable activity by being used for more than ten times, is simple in the preparation process and is environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of new biological materials, and in particular relates to a preparation method of magnetically immobilized lipase. Background technique [0002] Lipase as a biocatalyst is an enzyme that can catalyze organic reactions such as ester hydrolysis, alcoholysis, lipid synthesis or transesterification at the oil-water interface. Compared with chemical catalysts, lipase has the advantages of mild reaction conditions, low energy consumption, high product conversion rate, less side reactions, and environmental friendliness, so it is widely used in agriculture, food, medicine, and biodiesel. Although lipase has a wide range of potential applications in industry, most lipases are very sensitive to the environment and have problems such as short lifespan, insolubility of free enzyme in the organic phase, easy agglomeration of the reaction, difficulty in separating the product from the enzyme, and high price. , which gre...

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Problems solved by technology

[0008] In view of the poor lipophilicity in the prior art, the low pH value of the gel affects the activity of lipase, the relatively insufficient toughness of the prepared immobilized carrier, the high brittleness, and the large loss of grinding and screening in the later stage, resulting in partial product yield. Low, drying process is not conducive to problems such as industrial production, the application provides a kind of preparation method of magnetically immobilized lipase to solve the above-mentioned existing problems

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