Newcastle disease attenuated sugar vitrified vaccine and preparation method thereof

Abstract

The invention belongs to the technical field of veterinary drugs, and particularly relates to a Newcastle disease attenuated sugar vitrified vaccine and a preparation method thereof. The preparation raw materials of the vaccine include hatching eggs, reagents and seed virus. The reagents comprise any one or more of PBS (phosphate buffer solution) or normal saline and any one or more of a penicillin solution or a streptomycin solution; and the seed virus comprises any one or more of an HB1 strain, an F strain, a LaSota strain, a LaSota-Clone30 strain or an N79 strain, and propiolactone. The vaccine also comprises a raw material reactant for preparing the vitrified vaccine, and the reactant is specifically one or more of trehalose or agarose. According to the present invention, an erythrocyte experiment is directly performed on the generated vaccine, and the optimal attenuated virus can be found according to the optimal erythrocyte agglutination point so as to effectively ensure the performance of the vaccine.

Description

technical field [0001] The invention relates to the technical field of veterinary drugs, in particular to a Newcastle disease attenuated sugar vitrified vaccine and a preparation method thereof. Background technique [0002] Newcastle disease is an acute and highly contagious poultry infectious disease caused by Newcastle disease virus (The morbidity and mortality are increasing year by year, and there is no specific drug treatment, which has caused serious economic losses to the poultry industry. The World Organization for Animal Health (OIE) lists it as a Class A infectious disease, and my country lists Newcastle disease as a major animal disease and a Class I infectious disease. [0003] The method adopted for this virus in the prior art is inoculation prevention. As the drug resistance of the virus increases, the drug properties of the adopted vaccine gradually decrease after inoculation, causing the virus to still affect the growth of livestock, and in severe cases, it i...

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Problems solved by technology

[0003] In the prior art, the method adopted for this virus is inoculation prevention. As the drug resistance of the virus increases, the drug properties of the adopted vaccine gradually decrease after inoculation, causing the virus to still affect the growth of livestock, and it is easy to cause losses in severe cases.

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