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Application of indometacin to resistance to coronavirus infections

A coronavirus and indomethacin technology, applied in the field of drug research and development, can solve the problems of poor curative effect of SARS coronavirus infection and other problems

Inactive Publication Date: 2020-08-25
徐天宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In order to solve the problem of poor curative effect of drugs used to treat SARS coronavirus infection in the prior art, the present invention provides the application of indomethacin in SARS coronavirus infection, and provides a new dosage form of indomethacin , an inhaled dosage form, for the treatment of pneumonia caused by SARS coronavirus and its complications

Method used

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  • Application of indometacin to resistance to coronavirus infections
  • Application of indometacin to resistance to coronavirus infections
  • Application of indometacin to resistance to coronavirus infections

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1 anti-SARS-CoV infection pharmacodynamics experimental method

[0020] In the present invention, SARS-CoV (TOR-2) is used to infect human alveolar epithelial cell line A549. The cell monolayer was covered with a 12-well plate culture dish, and the virus yield was measured by PFU and TCID50. The A549 cells covered with a single layer were infected with SARS-CoV for 1 hour at 37 degrees Celsius, and the multiplicity of infection (MOI) was 10PFU / cell. One hour after virus infection, the virus inoculum was removed, washed with PBS three times, and indomethacin of different concentrations dissolved in DMSO was added, and the cell viability was detected by the MTT method. The results show that indomethacin can effectively inhibit the infection of SARS-CoV virus to host cells and reduce the production of virus particles. The inhibitory effect is positively correlated with the drug concentration, and its half inhibitory concentration (IC50) is 5 μM ( figure 2 ), a...

Embodiment 2

[0021] Embodiment 2 anti-SARS-CoV 2 infection pharmacodynamics experimental method

[0022] We used the SARS-CoV2 envelope S gene (SARS-CoV 2S, GenBank: MN908947.3, thanks to Tao Shengce's research group at the Institute of Systems Biomedicine, Shanghai Jiao Tong University for donating the plasmid) to co-transfect the plasmid expressing the S protein and the HIV core Plasmid (pNL4-3-Luc-R-E-), which can obtain the SARS-CoV 2 recombinant virus SARS-CoV 2S / HIV with the S protein as the shell wrapping the HIV core, the construction method is similar to the previous construction of the SARS-CoV pseudovirus [Ying Guo, etc. Identification of a New Region of SARS-CoV S Protein Critical for Viral Entry. Journal of Molecular Biology 2009,394:600-605.]. Due to the deletion of env, nef and vpr genes on the HIV vector, the virus can only enter the host cell but cannot replicate, so the virus is safe. At the same time, the vector has a luciferase reporter gene, so it can The degree of ce...

Embodiment 3

[0026] Embodiment 3 A kind of inhalation dosage form of indomethacin

[0027] Indomethacin is a commonly used non-corticosteroid anti-inflammatory, antipyretic and analgesic drug. At present, the main dosage forms are various oral dosage forms, topical liniments, suppositories, etc., and there is no inhalation dosage form. The invention provides an inhalation dosage form of indomethacin, which is used to increase the effective concentration of indomethacin in local lung cells, has higher bioavailability, avoids the first-pass effect of the liver, and more effectively treats SARS virus infection Pneumonia caused by other factors.

[0028]Indomethacin has poor water solubility. We add sodium carbonate to normal saline, adjust the pH value of the solution to 7.4, slowly add indomethacin to form indomethacin sodium salt, which can significantly enhance its water solubility, and configure it to 20mg / ML indomethacin nebulized inhalation solution, take 2ml of indomethacin nebulized...

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Abstract

The invention relates to application of indometacin to resistance to coronavirus infections, and provides new application of the indometacin to inhibition from the SARS coronavirus and a novel preparation of the indometacin. The novel preparation is an inhalation preparation. The indometacin can obviously inhibit the SARS coronavirus, and thus is expected to be a medicine for treating diseases caused by the SARS coronavirus.

Description

technical field [0001] The invention belongs to the field of drug research and development, specifically illustrating the application of indomethacin in anti-coronavirus infection. Background technique [0002] Coronavirus (Coronavirus) is a kind of pathogenic single-stranded RNA virus that infects humans and other vertebrates and causes respiratory and digestive tract inflammation. In terms of systematic classification, coronaviruses belong to the order Nidovirales, the family Coronaviridae, and the genus Coronavirus. They are a large class of viruses that exist widely in nature. The 2019 novel coronavirus (SARS-CoV-2) is currently the seventh known coronavirus that can infect humans. It is genetically very close to the SARS-CoV coronavirus that broke out in 2003. According to the International Virus Taxonomy Committee, it belongs to The same virus species (Table 1), the remaining 5 coronaviruses that infect humans are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, and MERS-C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/405A61P31/14A61P11/00
CPCA61K31/405A61P11/00A61P31/14
Inventor 徐天宏桑托罗
Owner 徐天宏
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