Preparation method of facial mask containing stem cell exosome components

A technology for mask preparation and exosomes, applied in the field of stem cell application, can solve the problems of insignificant mask effect, uneven material mixing, and reduced mask effectiveness, and achieve the effect of avoiding mutual infection, easy penetration and absorption, and ensuring activity.

Inactive Publication Date: 2020-08-21
河南省遗传资源细胞库有限公司 +1
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Problems solved by technology

[0004] In recent years, the application of stem cells in skin-tightening cosmetics has also become a research hotspot, especially in facial masks. However, in most existing facial masks, the human face does not absorb the substances in place, resulting in a less effective mask. It is not obvious, and some of the existing masks containing stem cell exosomes are unevenly mixed or incompletely sterilized during production, which will easily lead to a decline in the effectiveness of the mask

Method used

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  • Preparation method of facial mask containing stem cell exosome components

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] see figure 1 , a method for preparing a mask containing stem cell exosome components, comprising the following steps:

[0027] Step 1. Extracting mesenchymal stem cells, using adult mesenchymal stem cells extracted from blood for serum-free culture;

[0028] Step 2. Cultivate mesenchymal stem cells, culture the extracted mesenchymal stem cells in serum-free medium, and add serum probiotics, L-glutamine, deoxyribonucleoside, FGF and TGF-B to the medium ...

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Abstract

The invention discloses a preparation method of a facial mask containing stem cell exosome components. The preparation method comprises the following steps: extracting mesenchymal stem cells, culturing the mesenchymal stem cells, extracting stem cell exosomes, preparing a facial mask material and synthesizing the facial mask. The beneficial effects of the invention are that: the method is suitablefor large-scale production; accidents such as infection in the transportation process are avoided; meanwhile, the problem of mutual infection is also avoided; the integrity and high purity of the rawmaterials of the facial mask in the production process are ensured through twice filtration and advanced sterilization; the functionality of a mask is improved; bletilla striata, ligusticum chuanxiong hort, and lithospermum erythrorhizon are ground and crushed under a negative pressure condition to ensure that the raw materials bletilla striata, ligusticum chuanxiong hort, and lithospermum erythrorhizon are fully ground; the activity is not influenced by temperature, complete functions of the mask are guaranteed, the mask is convenient to prepare and reserve, a mixed chemical solution can guarantee full mixing of the raw materials, the activity of the materials is guaranteed, the materials cannot be lost, all the raw materials can play roles, the absorption efficiency of a user is improved, and maintenance substances can permeate and be absorbed more easily.

Description

technical field [0001] The invention relates to a method for researching stem cell exosomes, in particular to a method for preparing a mask containing stem cell exosome components, and belongs to the technical field of stem cell applications. Background technique [0002] Exosomes are nanoscale lipid bilayer vesicles released by exocytosis to the outside of cells, and can exist in various types of extracellular body fluids, such as blood, urine, amniotic fluid, saliva, cerebrospinal fluid, and even breast milk. It is found that exosomes contain cell-specific proteins, lipids and nucleic acids, which can be transmitted to other cells as signaling molecules, thereby changing the functions of other cells, such as: exosomes participate in neuron communication, antigen presentation, immune response, organ Regulates fundamental physiological processes such as development and reproduction, and is also involved in the regulation of pathological processes such as cancer progression, ...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/9794A61K8/9789A61K8/92A61K8/73A61K8/64A61K8/58A61K8/55A61K8/02A61K8/34A61K8/49A61Q19/00C12N5/0775
CPCA61K8/99A61K8/9789A61K8/9794A61K8/922A61K8/731A61K8/64A61K8/553A61K8/585A61K8/498A61K8/342A61K8/0212A61Q19/00C12N5/0665A61K2800/81A61K2800/84A61K2800/805C12N2500/40C12N2500/32C12N2501/113C12N2501/115C12N2501/15C12N2500/38
Inventor 朱学义韦丹
Owner 河南省遗传资源细胞库有限公司
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