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Application of alkaloid atropine in relieving low-temperature freeze injury stress of plant seedlings

A technology of atropine and alkaloids, which is applied in the field of agricultural plant production, can solve unclear problems, and achieve the effect of simple and easy to use, strong operability, and improved frost resistance

Active Publication Date: 2020-08-21
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Up to now, it is still unclear whether exogenous tropine alkaloids, as protective agents, can promote the abiotic resistance of rapeseed and Arabidopsis

Method used

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  • Application of alkaloid atropine in relieving low-temperature freeze injury stress of plant seedlings
  • Application of alkaloid atropine in relieving low-temperature freeze injury stress of plant seedlings
  • Application of alkaloid atropine in relieving low-temperature freeze injury stress of plant seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The application of alkaloid atropine in alleviating the freezing damage of rapeseed seedlings is as follows:

[0020] 1. Attach a layer of nylon mesh to a rectangular plastic box, tighten it with a rubber band, and place it in a hydroponic tank filled with 1 / 4 Hoagland's culture medium. The liquid surface touches the nylon mesh, and select full seeds. , arranged on a nylon net, placed in a culture room at 25°C, and cultivated for 1 week (16 hours of light / 8 hours of darkness), then pick rape seedlings with consistent growth, insert them into a plastic plate with holes, and immerse the roots in the culture solution Continue to cultivate for a week;

[0021] 2. Prepare an aqueous solution containing the alkaloid atropine according to Table 1. Atropine was purchased from Shanghai Mokai Biotechnology Co., Ltd., item number M18261, with a purity of 99%;

[0022] 3. The roots of the seedlings were immersed in liquids containing different concentrations of the alkaloid atropi...

Embodiment 2

[0028] The application of the alkaloid atropine in alleviating the low-temperature freezing injury stress of Arabidopsis thaliana is as follows:

[0029] 1. Disinfect the harvested Arabidopsis seeds, and then vernalize them in a refrigerator at 4°C for 3 days;

[0030] 2. Sow the Arabidopsis thaliana seeds in 1 / 2MS solid medium, put them in the light incubator, transfer to the culture medium after germination (vermiculite: nutrient soil is mixed in a volume ratio of 1:1), and cultivate for 1 month;

[0031] 3. Prepare control group and treatment group 1 and treatment group 2 according to Table 3, select seedlings with consistent growth, pour alkaloid aqueous solution of different concentrations at the root position of each plant seedling, and cultivate overnight, each processing 3 bowls, 3 times biological replicates;

[0032] 4. Put the seedlings in a -4°C incubator for 4 hours, and then transfer to normal growth conditions to recover for 3 days.

[0033] The results found ...

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Abstract

The invention discloses an application of alkaloid atropine in relieving low-temperature freeze injury stress of plants. The cold resistance of the plants can be improved through exogenous alkaloid atropine when cold injury occurs. Under the low-temperature stress condition, an alkaloid atropine aqueous solution with the concentration of 50-150 nmol / plant can be set for rape seedling root soakingtreatment, and freezing injury stress of rape is relieved; and under the low-temperature stress condition, an alkaloid atropine aqueous solution with the concentration of 10-30 nmol / plant can be set for arabidopsis thaliana seedling irrigation treatment, and the effect of relieving freezing injury stress of arabidopsis thaliana is achieved. Alkaloid atropine treatment is adopted to relieve damageof low temperature to the plant seedling stage, and a new way is opened up for guaranteeing safe overwintering of oilseed rape seedlings.

Description

technical field [0001] The invention belongs to the field of agricultural plant production, and more specifically relates to the application of the alkaloid atropine in alleviating the stress of plant low-temperature freezing damage. Background technique [0002] Rapeseed is China's fifth largest crop after rice, wheat, corn, and soybeans. It shoulders the heavy responsibility of domestic vegetable oil supply. Therefore, the development of rapeseed industry is an important part of my country's agricultural production, and it is also of great significance to the security of national oil supply. . [0003] In the Yangtze River Basin, the main production area of ​​winter rapeseed, light and simplified cultivation and mechanized planting are currently advocated in production, and the area of ​​direct seeding rape is expanding year by year. However, the direct seeding planting method of rapeseed cannot fully adapt to the planting mode of rice and oil rotation in the Yangtze River...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01P21/00A01G22/15A01G22/00A01G13/00
CPCA01G13/00A01N43/90A01G22/00A01G22/15
Inventor 吕艳黄涌罗丹张学昆程勇邹锡玲陆光远
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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