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Human respiratory tract epithelial cell pathogen cytology detection method and kit

A technology of epithelial cells and detection methods, applied in measuring devices, scientific instruments, instruments, etc., can solve problems such as large differences in viruses, undisclosed antigen and antibody detection methods, etc.

Pending Publication Date: 2020-07-28
苏州百道医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0010] The invention patent of Chinese Patent Publication No. CN1695057 discloses a disease antigen detection technology based on nucleic acid probes. However, the detection methods for each disease should be different based on the characteristics of the disease, which is not disclosed in the patent. Based on the detection method of antigen and antibody in the detection method of epithelial cells of human respiratory diseases, the detection results will be more accurate by combining nucleic acid probes with antibodies
[0011] Chinese patent publication number is CN103604922 invention patent, although a kind of respiratory syncytial virus kit based on enzyme immunoassay technology is disclosed, but its final result judgment is still based on the numerical value, and directly observed in the cell in the cytology detection Viruses are quite different, cytological observation will be more clear

Method used

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  • Human respiratory tract epithelial cell pathogen cytology detection method and kit
  • Human respiratory tract epithelial cell pathogen cytology detection method and kit
  • Human respiratory tract epithelial cell pathogen cytology detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] This embodiment provides a general cytological detection method for human upper respiratory tract epithelial cell pathogens, comprising the following steps:

[0095] S1. Specimen collection and preparation:

[0096] Collect upper respiratory tract specimens such as nasopharynx, oral epithelial cells or sputum, put them in the cell preservation solution in the sampling tube and fix them, and then prepare sample slides. The methods include but are not limited to the following two:

[0097] a. Cell manual smear: collect the cells in the sampling tube for cell smear and cell fixation;

[0098] b. Automatic cell smear: automatic liquid-based thin-layer cell film maker to prepare sample slides and fix cells;

[0099] S2, ICC immunocytochemical staining technique (abbreviation, antibody detection)

[0100] Methods include but are not limited to the following two, as follows:

[0101] a. Instrument testing:

[0102] Put the reagents and prepared samples into the automatic imm...

Embodiment 2

[0134] The influence of embodiment two cell preservation solution on fixation effect

[0135] It should be noted that different cell preservation solutions have a greater impact on the fixation effect of the sample. This example provides comparative test data on the influence of different cell preservation solutions on the fixation effect, as follows:

[0136] 1. The effect of cell preservation solution on the number of cells

[0137] Take 6x10 7 Remove the supernatant of the 293T cells, add PBS to resuspend, divide into six parts on average, as samples 1, 2, 3, 4, 5, and 6, repeat at least 3 times for each sample, and calculate the average value of the experimental results. Centrifuge again, remove the supernatant, and add samples 1, 2, 3, 4, 5, and 6 respectively:

[0138] Sample 1: 1% mass fraction of paraformaldehyde, 75% volume fraction of ethanol

[0139] Sample 2: 1% mass fraction of paraformaldehyde, 60% volume fraction of ethanol

[0140] Sample 3: 1% mass fractio...

Embodiment 3

[0174] Embodiment 3 The steps and logic of computer automatic judgment positive

[0175] This embodiment provides a method based on computer artificial intelligence technology to automatically count pictures in the server and identify positive cells, and output an auxiliary diagnosis report, as follows:

[0176] S1. Collect the types of pictures that need to be interpreted, including negative and positive, and sample pictures from different parts, and perform cell labeling.

[0177] S2. Carry out model training and evaluate the accuracy of the model. Review the data annotation, continue training, and optimize the algorithm.

[0178] S3. When the accuracy of the model meets expectations, the test can be performed.

[0179] See specific steps figure 2 , see the output picture result image 3 . Since the computer automatic judgment technology is not the key point of the present invention, it will not be described in detail here.

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Abstract

The invention relates to the field of human respiratory tract epithelial cell pathogen detection, in particular to a human respiratory tract epithelial cell pathogen cytology detection method and a kit. The method comprises the following steps of: S1, sample acquisition: collecting human respiratory tract epithelial cells, and fixing the human respiratory tract epithelial cells in a cell preservation solution; S2, flaking: manually or automatically adding a sample onto a sample carrying medium; S3, incubating: incubating the sample by adopting an immune cell chemical staining technology and / ora chromogenic in-situ hybridization technology; and S4, positive sample judgment: manually counting and identifying positive cells and outputting a diagnosis report, or automatically counting and outputting an auxiliary diagnosis report after computer scanning. With the human respiratory tract epithelial cell pathogen cytology detection method and the kit adopted, the defects of an existing diagnosis means, such as nucleic acid loss in a sample extraction process in nucleic acid detection can be eliminated; and a virus detection rate can be improved. The method is more sensitive, specific andefficient than nucleic acid detection, CT and other serological detection products which are conventionally used at present and serological test products which are under development.

Description

technical field [0001] The invention relates to the field of human respiratory epithelial cell pathogen detection, in particular to a human respiratory epithelial cell pathogen cytological detection method and a kit. Background technique [0002] Early diagnosis of the disease can greatly increase the patient's chance of survival. Early detection and early diagnosis are the key to improving the cure rate and reducing the fatality rate. In vitro diagnostic products can be mainly divided into: biochemical diagnostic products, immunodiagnostic products, molecular diagnostic products, microbiological diagnostic products, urine diagnostic products, blood coagulation diagnostic products, hematology and flow cytometric diagnostic products, on-site Rapid diagnostic products and other eight categories, of which biochemical, immune, molecular diagnosis and on-site rapid diagnostic products are the main four categories of domestic diagnostic products. [0003] Taking human respirator...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983
Inventor 吴纯宋砚明刘杨刘岩孙晓磊郭金城周勤王超
Owner 苏州百道医疗科技有限公司
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