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Preparation method and application of icariin sustained-release nano-gel by aerosol targeted drug delivery

A technology of icariin and nano-gel is applied in the field of medicine to achieve the effects of reducing asthmatic airway inflammatory response, good bioadhesion, and reducing the level of cytokines

Pending Publication Date: 2020-07-24
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of icariin-zinc alginate nanogel and its preparation method for the treatment of asthma with rapid effect of ultrasonic nebulization inhalation in view of the technical problems existing in the existing medicine for treating asthma and its preparation method

Method used

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  • Preparation method and application of icariin sustained-release nano-gel by aerosol targeted drug delivery
  • Preparation method and application of icariin sustained-release nano-gel by aerosol targeted drug delivery
  • Preparation method and application of icariin sustained-release nano-gel by aerosol targeted drug delivery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of icariin-alginate nanogel

[0043] 1.1 Preparation of sodium alginate-water solution

[0044] At 45°C (usually 40-50°C), mix sodium alginate with water evenly to make a sodium alginate-water solution with a mass-volume concentration of 0.50% (m / v, usually 0.25-0.75%) ;

[0045] 1.2 Preparation of zinc chloride-ethanol solution

[0046] Mix zinc chloride with an ethanol solution with a concentration of 60% (usually 50-70%) by volume and vortex to prepare a chlorine concentration of 0.1% (m / v, usually 0.1-0.2%) Zinc chloride-ethanol solution;

[0047] 1.3 Preparation of icariin-zinc chloride solution

[0048] Add icariin into the zinc chloride-ethanol solution, and vortex mix, wherein, the ratio of the mass of icariin to the volume of the zinc chloride-ethanol solution is 0.3:100 (usually (0.1- 0.4): 100), that is, 0.3 g (usually 0.1-0.4 g) of icariin is added to 100 mL of zinc chloride-ethanol solution to prepare an icariin-zinc chloride solu...

Embodiment 2

[0055] Embodiment 2 Characterization of icariin-zinc alginate nanogel

[0056] 2.1 Particle size and Zeta potential test

[0057] The prepared zinc alginate nanogel solution and icariin-zinc alginate nanogel solution were diluted 10 times, and the particle size, particle size distribution and surface Zeta potential of the nanogel were measured using a nanoparticle size analyzer. The measurement was repeated 3 times, and the results were averaged, and the test results are shown in Table 1.

[0058] Table 1 Particle size and Zeta-potential (Means±SD) of zinc alginate nanogel

[0059]

[0060]

[0061] 2.2 Fourier transform infrared spectroscopy analysis

[0062] Sodium alginate, icariin, mixture of icariin-zinc chloride-sodium alginate, zinc alginate nanogel and icariin-zinc alginate nanogel dried to constant weight were mixed with bromine Potassium chloride mixed tablet, with the blank potassium bromide tablet as a control, the infrared spectrum of the sample is meas...

Embodiment 3

[0064] Example 3 Icariin-Zinc Alginate Nanogel Drug Loading Efficiency and Encapsulation Efficiency

[0065] Preparation of drug loading rate sample solution: Accurately weigh 3 parts of icariin-zinc alginate nanogel, 25.00 mg each, add an appropriate amount of methanol to the sample for ultrasonic extraction several times, extract icariin monomer to constant volume In a 25mL volumetric flask, after standing still, take the supernatant and filter it with a 0.45μm pore size microporous membrane, and determine the content of icariin by HPLC.

[0066] Preparation of the encapsulation efficiency sample solution: Accurately weigh 3 parts of the same batch of icariin-zinc alginate nanogel, 25.00 mg per part, add an appropriate amount of ultrapure water, and after ultrasonic treatment for 30 minutes, set the volume in a 25 mL volumetric flask. After standing still, take the supernatant and add it to the ultrafiltration tube. After centrifugation, keep the solution in the sleeve for u...

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Abstract

The invention discloses a sustained-release nano-gel by aerosol targeted drug delivery for treating respiratory system diseases. Sodium alginate high-molecular polymer is used as a vector material toconstruct a zinc alginate nano-gel drug delivery system to serve as a drug release storage cavern. Icariin-zinc alginate nano-gel is delivered to the lung in a targeted manner by adopting a drug delivery mode of ultrasonic aerosol inhalation, so that the liver first-pass effect of drugs can be avoided, response is rapid, and clinical drug delivery characteristics are accorded. The icariin-zinc alginate nano-gel can be directly and quickly distributed to the whole lung after ultrasonic aerosol inhalation, and can be adhered to the lung surface to constantly and stably release the icariin, so that the bioavailability of drugs can be improved, and the effect of treating respiratory system diseases can be achieved. Therefore, a proper and efficient novel preparation is provided to icariin treated respiratory system diseases, and targeted drug delivery can be performed through ultrasonic aerosol inhalation, and the application range of the sustained-release nano-gel can be enlarged.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method of icariin sustained-release nanogel for atomized targeted administration, which is used for preventing, alleviating and / or treating respiratory diseases, including bronchial asthma, chronic obstructive Lung disease, bronchitis and other diseases. Background technique [0002] Bronchial asthma (bronchial asthma), referred to as asthma, is a chronic airway inflammatory respiratory disease involving eosinophils, mast cells, lymphocytes, airway epithelial cells and other inflammatory cells and their cellular components. It is one of the common and frequently-occurring diseases that threaten public health worldwide. Its typical features are persistent airway inflammation, reversible airway obstruction, airway remodeling and airway hyperresponsiveness, clinical manifestations are wheezing, shortness of breath, chest tightness and coughing. According to the latest...

Claims

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Application Information

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IPC IPC(8): A61K47/61A61K9/19A61K9/12A61K31/7048A61K33/30A61P11/06
CPCA61K47/61A61K9/0075A61K9/19A61K31/7048A61K33/30A61P11/06A61K9/12A61K2300/00
Inventor 王强松岳晓月崔元璐吴佳蓉
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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