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A kind of bpns/chitosan/prp thermosensitive hydrogel and its preparation method and application

A temperature-sensitive hydrogel and chitosan technology, which can be used in pharmaceutical formulations, allergic diseases, photodynamic therapy, etc., can solve the problems of limiting the application of PDT and PTT, and achieve the removal of hyperplastic synovial tissue, low cytotoxicity, and cell affinity and proliferative ability of good effect

Active Publication Date: 2022-03-11
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of PDT and PTT in the treatment of RA is limited due to the effective requirement of removal of hyperplastic synovial tissue and concurrent osteogenesis

Method used

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  • A kind of bpns/chitosan/prp thermosensitive hydrogel and its preparation method and application
  • A kind of bpns/chitosan/prp thermosensitive hydrogel and its preparation method and application
  • A kind of bpns/chitosan/prp thermosensitive hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Preparation of BPNs / chitosan / PRP thermosensitive hydrogel

[0054] BPNs / chitosan / PRP thermosensitive hydrogel provided by the invention

[0055] (1) Grind black phosphorus crystals into powder in a grinder. Black phosphorus crystal powder was ultrasonically treated at 1200W for 3h, then centrifuged at 4000rpm for 10min to obtain BPNs, which were stored and sealed at 4°C.

[0056] (2) 200 mg of chitosan (90% deacetylation degree) was dissolved in 9 mL of 0.1 mol / L acetic acid solution to prepare chitosan acetic acid solution. Dissolve 560 mg of β-GP in 1 mL of deionized water to prepare an aqueous solution of β-GP. Under uniform stirring, the β-GP aqueous solution was added dropwise to the chitosan acetic acid solution to obtain a clear and uniform chitosan thermosensitive hydrogel, which was stored and sealed at 4 °C.

[0057] (3) Mix the BPNs (200 μg / mL, 1.25 mL) prepared in step (1) with the chitosan thermosensitive hydrogel (4.75 mL) prepared in step (2), and t...

Embodiment 2

[0086] Example 2 Therapeutic experiment of BPNs / chitosan / PRP thermosensitive hydrogel on RA at the cellular level

[0087] 1. Materials

[0088] 1.1 Reagents

[0089]

[0090]

[0091] 1.2 Instruments

[0092]

[0093] 2. Experimental method

[0094] In order to study the ROS production of PRP-chitosan thermosensitive hydrogel combined with black phosphorus nanosheets injectable biomaterials and the scavenging effect on rheumatoid synoviocytes, the inventors combined PRP-chitosan thermosensitive hydrogel at the cellular level A range of properties of black phosphorus nanosheets as an injectable biomaterial were investigated.

[0095] 2.1 Culture of rheumatoid synoviocytes

[0096] (1) The configuration of the culture medium:

[0097] Take 1000mL of DMEM medium, add 10% fetal bovine serum, 1% penicillin and streptomycin, 1% L-glutamine, filter and sterilize, and package in a 50ml cell-specific centrifuge tube for later use.

[0098] (2) Cryopreservation and recov...

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Abstract

The invention relates to a BPNs / chitosan / PRP temperature-sensitive hydrogel and a preparation method and application thereof. The present invention mainly mixes BPNs, chitosan thermosensitive hydrogel and activated PRP to prepare BPNs / chitosan / PRP thermosensitive hydrogel, through phototherapy of BPNs, biotherapy treatment of platelet-rich plasma (PRP) and phosphorus-driven Calcium extracts biomineralization for proliferation, so that it has good PTT and PDT effects, low cytotoxicity in different cells, and a survival rate higher than 85%; good biocompatibility, cell affinity and proliferation ability; in near-infrared light It is easy to generate ROS under irradiation, and reaches the maximum within 8 minutes, which can promote cell adhesion, growth and extracellular matrix generation; it can effectively remove hyperplastic synovial tissue, repair defective bone tissue and maintain the smoothness of the articular surface, and can be used as a photothermal and / or photodynamic drugs for the treatment of rheumatoid arthritis.

Description

technical field [0001] The invention belongs to the field of hydrogel technology, and in particular relates to a BPNs / chitosan / PRP temperature-sensitive hydrogel and a preparation method and application thereof. Background technique [0002] Rheumatoid arthritis (RA) is the most common chronic systemic inflammatory disease, affecting about 1% of the world's population, resulting in long-term arthritis, cartilage degeneration, bone defects, disability and premature death. Therapies for RA can cause many adverse side effects, including infection, interstitial lung disease and impaired glucose metabolism. The disadvantage of the operation is that it has a large trauma, extensive adhesion after the operation, and cannot be reused. Despite the advantages of arthroscopic synovectomy over conventional surgery, blind spots remain. Chemical synovectomy and radiosurgical resection may damage normal tissue. [0003] In recent years, there has been increasing focus on phototherapy in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/06A61K47/36A61P19/02A61P37/02A61P19/08A61K35/16A61K35/19
CPCA61K9/06A61K47/36A61K35/19A61K41/0057A61K41/0052A61K35/16A61P19/02A61P19/08A61P37/02A61K9/0009A61K2300/00
Inventor 高丰雷潘文振郭开金
Owner XUZHOU MEDICAL UNIV
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