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Biological deodorizing preparation and preparation method and application thereof

A biological deodorization and preparation technology, applied in the field of animal husbandry engineering, can solve the problems of corrosiveness, affecting human health, hindering the absorption of nutrients, etc., and achieve the effects of good ammonia emission, obvious industrialization prospects, and labor cost saving

Active Publication Date: 2020-06-19
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These gaseous substances not only affect human health, but also are easily adsorbed on the mucous membrane of the respiratory tract, and are corrosive. In the long run, they will cause damage to the mucous membrane, resulting in poor epidemic prevention of pigs and easy to get sick
At the same time, after ammonia gas enters the body, it will hinder the absorption of nutrients, and will make the living environment of germs more comfortable, breed bacteria, and cause great losses to animal husbandry farms. Therefore, the sustainable development of animal husbandry production requires a High-efficiency, low-cost solution to reduce ammonia and other odor emissions
[0003] At present, there are some deodorization methods for farms that rely on physical adsorption and other methods, but these methods require a large cost of supporting infrastructure and are difficult to effectively promote
However, there are still few types of microbial deodorants or functional feed preparations aimed at reducing and removing the odor of farms, with narrow user options, single use methods, and unsatisfactory use effects. Therefore, it is urgent to develop a high-efficiency, low-efficiency Cost-effective, safe biological deodorizers or deodorizing feed additives reduce ammonia and odor emissions at the source

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] Example of product preparation

[0023] 1. Preparation medium

[0024] 1. Medium

[0025] 1.1 Seed medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L.

[0026] 1.2 Basic fermentation medium: disodium hydrogen phosphate 8.5g / L, potassium dihydrogen phosphate 3g / L, ammonium chloride 1g / L, sodium chloride 0.5g / L, brown sugar 50g / L, magnesium sulfate heptahydrate 0.492g / L L, where the pH value is 6.8-7.0.

[0027] 1.3 Instruments and equipment: temperature-controlled shaker, autoclave, electronic balance, sterile operating table, ultraviolet spectrophotometer

[0028]2. Preparation of Bacillus methylotrophicus (Bacillus methylotrophicus) GBacillus-9 strain fermentation broth

[0029] 2.1 Culture method

[0030] 2.1.1 Strain preservation method: Mix the bacterial solution when the OD600 of the seed medium reaches 1.5 and 50% sterile glycerol in a 1:1 ratio in a 1.5ml EP tube, seal it with a parafilm, and store it in a -20°C refrigerator, long-term storage...

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PUM

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Abstract

The invention relates to a biological deodorizing preparation. The biological deodorizing preparation is characterized in that an active component is a Bacillus methylotrophicus GBacillus-9 strain. Pasture test results show that the biological deodorizing preparation disclosed by the invention can notably reduce the concentration of ammonia in pens of pig farms, and has broad application prospects. The biological deodorizing preparation has favorable effects in reducing discharge of the ammonia in cultivation farms and particularly the pig farms. The raw materials of the biological preparationproduct, namely the biological deodorizing preparation, disclosed by the invention are easy to obtain, and the biological deodorizing preparation is simple in technology and suitable for large-scaleproduction; and besides, the biological preparation disclosed by the invention can be used in simple manners of spraying, adding to drinking water for animals, and / or adding to feeds and the like, andlabor cost and capital construction cost can be effectively saved.

Description

technical field [0001] The invention belongs to the field of animal husbandry engineering, and in particular relates to a biological deodorizing preparation suitable for livestock and poultry farms and its preparation and application. Background technique [0002] As the country continues to introduce more and more stringent policies and regulations on livestock producers, environmental protection has become the biggest production problem faced by farms, and among many farms, the environmental protection problem of pig farms is the most prominent. Gases such as ammonia and hydrogen sulfide emitted by pigs and pig manure are the main sources of odor in pig farms. Because the environment is not up to standard, the surrounding people have strong reactions, and farms have been shut down for renovation from time to time. These gaseous substances not only affect human health, but are also easily adsorbed on the mucous membrane of the respiratory tract and are corrosive. In the lon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/58A23K10/18C12R1/07
CPCC12N1/20B01D53/84B01D53/58A23K10/18B01D2257/406B01D2258/0266Y02A50/20
Inventor 石恒波林国栋吕正兵舒建洪杨一凡娄港归方银兵
Owner ZHEJIANG SCI-TECH UNIV
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