Biological deodorizing preparation and preparation method and application thereof
A biological deodorization and preparation technology, applied in the field of animal husbandry engineering, can solve the problems of corrosiveness, affecting human health, hindering the absorption of nutrients, etc., and achieve the effects of good ammonia emission, obvious industrialization prospects, and labor cost saving
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[0022] Example of product preparation
[0023] 1. Preparation medium
[0024] 1. Medium
[0025] 1.1 Seed medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L.
[0026] 1.2 Basic fermentation medium: disodium hydrogen phosphate 8.5g / L, potassium dihydrogen phosphate 3g / L, ammonium chloride 1g / L, sodium chloride 0.5g / L, brown sugar 50g / L, magnesium sulfate heptahydrate 0.492g / L L, where the pH value is 6.8-7.0.
[0027] 1.3 Instruments and equipment: temperature-controlled shaker, autoclave, electronic balance, sterile operating table, ultraviolet spectrophotometer
[0028]2. Preparation of Bacillus methylotrophicus (Bacillus methylotrophicus) GBacillus-9 strain fermentation broth
[0029] 2.1 Culture method
[0030] 2.1.1 Strain preservation method: Mix the bacterial solution when the OD600 of the seed medium reaches 1.5 and 50% sterile glycerol in a 1:1 ratio in a 1.5ml EP tube, seal it with a parafilm, and store it in a -20°C refrigerator, long-term storage...
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