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Method for determining content of panax japonicus saponin IVa in throat clearing oral liquid

A technology of bamboo ginseng saponins and determination methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of inaccurate control of the quality, accuracy, stability or precision of Tu Nixie and Houyanqing oral liquid and other problems, to achieve the effect of low cost, high precision and accuracy, and simple sample processing

Inactive Publication Date: 2020-06-05
湖南时代阳光药业股份有限公司
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  • Claims
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Problems solved by technology

One of the disadvantages of this method is that the thin-layer chromatography scanning method is used for quantitative experiments, which is not good in terms of accuracy, stability or precision. The use of thin-layer chromatography scanning method for quantitative research has been gradually cancelled.
In addition, the oleanolic acid obtained by hydrolysis is the aglycone of various saponins rather than the single active index component of the monarch drug Tu Nixie in Houyanqing Oral Liquid, so it is impossible to accurately control the quality of Tu Nixie and Houyanqing Oral Liquid

Method used

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  • Method for determining content of panax japonicus saponin IVa in throat clearing oral liquid
  • Method for determining content of panax japonicus saponin IVa in throat clearing oral liquid
  • Method for determining content of panax japonicus saponin IVa in throat clearing oral liquid

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Experimental program
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Effect test

Embodiment 1

[0020] 1. Chromatographic conditions

[0021] Shimadzu HPLC instrument (LC-20A binary high-pressure gradient instrument, SPD-20A ultraviolet detector), chromatographic column is Shimadzu YMC C 18 Column (250mm×4.6mm, 5μm), detection wavelength 203nm, column temperature is room temperature, mobile phase is acetonitrile-0.5% phosphoric acid aqueous solution (33:67, V / V), flow rate is 1mL / min.

[0022] 2. Sample preparation

[0023] 1) Preparation of bamboo ginseng saponin IVa reference substance solution

[0024] Accurately weigh 3.0mg of bamboo ginseng saponin IVa (Chengdu Pusi Biotechnology Co., Ltd., purity greater than 98%), dissolve the mobile phase in a 10mL volumetric flask, and pass through a 0.45μm microporous membrane to obtain 0.30mg / mL of bamboo ginsenoside IVa reference substance solution.

[0025] 2) Preparation of oral liquid test solution

[0026] After the Houyanqing Oral Liquid is fully shaken, draw 2mL precisely, put it in a 10mL volumetric flask, add mob...

Embodiment 2

[0070] Adopt high performance liquid chromatograph (ultraviolet detector), chromatographic column is C 18 Column (250mm×4.6mm, 5μm), detection wavelength 203nm, column temperature room temperature, flow rate 1mL / min, mobile phase isocratic elution of acetonitrile-0.5% phosphoric acid aqueous solution (31.5:68.5, v / v). The sample was processed by diluting the oral solution 5 times with the mobile phase, passing it through a 0.45um microporous membrane, and injecting 20 μL of the sample. The methodological investigation results show that the retention time of bamboo ginseng saponin IVa is about 55 minutes, and it is separated from the adjacent peak baseline without interference, and the resolution is greater than 1.5; the reference substance has good linearity in the range of 0.15-3.0 μg, and the linear equation is y=298454x -15712, R 2 =0.9992; the precision of the instrument is good, and the RSD is 1.12%; the method reproducibility is good, and the RSD is 1.63%; the test prod...

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Abstract

The invention provides a method for determining the content of panax japonicus saponin IVa in a throat clearing oral liquid, HPLC is used for detection, and chromatographic conditions are as follows:a chromatographic column C18 is adopted, the detection wavelength is 200-210 nm, the column temperature is 20-40 DEG C, the flow rate is 0.8-1 mL / min, and a mobile phase is subjected to isocratic elution with an acetonitrile-phosphoric acid aqueous solution. The method has the advantages of simplicity and convenience in sample treatment, high precision and accuracy, good stability, low cost and the like, and lays a foundation for quality control of the laryngopharynx clearing oral liquid.

Description

technical field [0001] The invention belongs to the field of drug quality testing, and in particular relates to a method for determining the content of ginseng saponin IVa in Houyanqing oral liquid. Background technique [0002] "Houyanqing Oral Liquid" is derived from folk secret recipes. It is composed of four medicinal flavors: Achyranthes bidentata, Malancao, Tianmingjing and Plantain. It is a national-level new drug independently developed by Hunan Times Sunshine Pharmaceutical Co., Ltd. It has heat-clearing The effect of detoxification and sore throat is mainly used for throat swelling and pain, fever, thirst, constipation, tonsillitis, pharyngitis and other syndromes caused by excess heat in the lung and stomach. Achyranthes bidentata Blume is the wild species of Achyranthes bidentata Blume, Achyranthes longifolia (Makino) Makino, Achyranthes aspera L., Achyranthes aspera L. Roots and rhizomes of var. indica L. It has the effects of promoting blood circulation and r...

Claims

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Application Information

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IPC IPC(8): G01N30/89
CPCG01N30/89
Inventor 欧阳文于炜民曾颖陈韬刘梓琛胡云舒唐琴张云坤李顺祥唐纯玉王雄龙陈林郭丽娟
Owner 湖南时代阳光药业股份有限公司
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