Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A quick way to identify ag + Molecular logic gate with three-phase output function of and cys and its construction method

A logic gate, three-phase technology, applied in the field of molecular logic gate with three-phase output function and its construction, can solve problems such as low cost, achieve the effects of improving accuracy and sensitivity, improving sensitivity, reducing complexity and detection cost

Active Publication Date: 2021-09-28
SHANXI UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the limitations of the single signal output of existing molecular logic gates, and provides a method for quickly identifying Ag + A molecular logic gate and a construction method thereof with a three-phase output function of Cys, the signal output in the construction method of the molecular logic gate is a combined output of fluorescence intensity, fluorescence anisotropy, and fluorescence attenuation three-phase signals, providing more reliable and sensitive At the same time, the nucleic acid aptamer does not need to be labeled, the analysis and detection are simple and the cost is lower

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A quick way to identify ag <sup>+</sup> Molecular logic gate with three-phase output function of and cys and its construction method
  • A quick way to identify ag <sup>+</sup> Molecular logic gate with three-phase output function of and cys and its construction method
  • A quick way to identify ag <sup>+</sup> Molecular logic gate with three-phase output function of and cys and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The first step is to prepare berberine mother liquor:

[0049] Weigh 74.3mg of berberine hydrochloride, and dilute it with secondary deionized water in a 100 mL volumetric flask;

[0050] In the second step, Ag + -Preparation of nucleic acid aptamer solution:

[0051] Ag + - The aptamer was centrifuged for 5-10min, and the centrifuged Ag + -Nucleic acid aptamer was dissolved in 20 mmol / L Tris-HAc buffer to prepare Ag with a concentration of 100 μM + -Nucleic acid aptamer solution, heated at 90°C for 7 minutes, cooled to room temperature, stored at -20°C for later use, and diluted to 10 μM with 20 mmol / L Tris-HAc buffer before use;

[0052] The third step is to prepare Ag with different concentration gradients + Ionic standard solution:

[0053] Weigh 67.9 mg of silver nitrate, and dilute it in a 100 mL volumetric flask with secondary deionized water;

[0054] The fourth step is to prepare Cys standard solutions with different concentration gradients:

[0055] We...

Embodiment 2

[0070] The first step is to prepare berberine mother liquor:

[0071] Weigh 74.3mg of berberine hydrochloride, and dilute it with secondary deionized water in a 100 mL volumetric flask;

[0072] In the second step, Ag + -Preparation of nucleic acid aptamer solution:

[0073] Ag + - The aptamer was centrifuged for 5-10min, and the centrifuged Ag + -Nucleic acid aptamer was dissolved in 20 mmol / L Tris-HAc buffer to prepare Ag with a concentration of 100 μM + -Nucleic acid aptamer solution, heated at 90°C for 7 minutes, cooled to room temperature, stored at -20°C for later use, and diluted to 10 μM with 20 mmol / L Tris-HAc buffer before use;

[0074] The third step is to prepare Ag with different concentration gradients + standard solution:

[0075] Weigh 67.9 mg of silver nitrate, dilute it in a 100 mL volumetric flask with secondary deionized water, and dilute step by step to obtain standard solutions of different concentrations;

[0076] The fourth step is to prepare Cys...

Embodiment 3

[0095] The first step is to prepare berberine mother liquor:

[0096] Weigh 74.3mg of berberine hydrochloride, and dilute it with secondary deionized water in a 100 mL volumetric flask;

[0097] In the second step, Ag + -Preparation of nucleic acid aptamer solution:

[0098] Ag + - The aptamer was centrifuged for 5-10min, and the centrifuged Ag + -Nucleic acid aptamer was dissolved in 20 mmol / L Tris-HAc buffer to prepare Ag with a concentration of 100 μM + -Nucleic acid aptamer solution, heated at 90°C for 7 minutes, cooled to room temperature, stored at -20°C for later use, and diluted to 10 μM with 20 mmol / L Tris-HAc buffer before use;

[0099] The third step is to prepare Ag with different concentration gradients + standard solution:

[0100] Weigh 67.9 mg of silver nitrate, dilute it in a 100 mL volumetric flask with secondary deionized water, and dilute step by step to obtain standard solutions of different concentrations;

[0101] The fourth step is to prepare Cys...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A rapid identification of Ag + The molecular logic gate and the construction method thereof of the three-phase output function of Cys belong to the field of molecular computer technology, which can solve the limitation problem of the single signal output of the existing molecular logic gate, including: using Cys and Ag + As two parallel input signals, the output signals are fluorescence intensity, fluorescence anisotropy and fluorescence lifetime. With berberine as the signal reporting unit, Ag + ‑Nucleic acid aptamer is a molecular recognition substrate, using berberine in Ag + ‑Aptamer and Cys, Ag + The changes of fluorescence intensity, fluorescence anisotropy and fluorescence attenuation in different microenvironments when it exists, construct the basic Boolean logic gate of "forbidden" gate. The constructed molecular logic gate circuit has good performance, high sensitivity, and is easy to distinguish, and can solve the limitation brought by a single output signal.

Description

technical field [0001] The invention belongs to the technical field of molecular computers, and in particular relates to a rapid identification of Ag based on nucleic acid aptamer-target interaction and metal ion coordination. + Molecular logic gates with three-phase output function of Cys and its construction method. Background technique [0002] As a chemical system simulating logic operations, molecular logic gates realize the miniaturization of information processing and integration through computation at the molecular level. Similar to logic gates in digital circuits, like silicon processors that use electronic motion, to create micro-systems that can use the inherent diversity of molecules to complete computing functions, use molecules to describe the input and output signals of logic gates, and then realize Logical operations at the molecular level. Molecules in molecular logic gates can respond to external stimuli, and molecular logic gates also follow the rules of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/06G06N3/00
CPCC09K11/06G01N21/6428G01N2021/6439G06N3/002
Inventor 卫艳丽兰艺凤秦国杰王丽董川
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com