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Amino acid composition beneficial to proliferation of lactobacillus acidophilus and application thereof

A technology of Lactobacillus acidophilus and amino acids, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problem that the production capacity cannot meet the demand for the number of Lactobacillus acidophilus, and achieve the effect of meeting the demand

Active Publication Date: 2020-04-14
郑州和合生物工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The production level of cultivating Lactobacillus acidophilus using the existing culture medium is: the live bacteria content of the fermentation broth is about (1.0~2.0)×10 9 cfu / mL, the live bacteria content of freeze-dried bacteria powder is about 2.0×10 11 cfu / g; this production capacity cannot meet the existing market demand for the quantity of Lactobacillus acidophilus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example relates to an amino acid composition that is beneficial to the proliferation of Lactobacillus acidophilus. The amino acid composition includes the following components in weight percentage: 60% alanine, 30% phenylalanine and 10% methionine. The composition is directly added to the MRS basic fermentation medium as a growth factor in the basic fermentation medium; the usage amounts of alanine, phenylalanine and methionine in the MRS basic fermentation medium are all 5g-50g / ton.

[0023] The application of the amino acid composition beneficial to the proliferation of Lactobacillus acidophilus in a fermentation process includes the following application steps:

[0024] 1) Prepare the basic fermentation medium of Lactobacillus acidophilus MRS, weigh the components of the basic fermentation medium according to the production scale of 5 tons: glucose 100kg, peptone 50kg, beef extract 50kg, yeast extract 25kg, sodium acetate 25kg, dihydrogen citrate Ammonium 12.5kg...

Embodiment 2

[0030] This example relates to an amino acid composition that is beneficial to the proliferation of Lactobacillus acidophilus. The amino acid composition includes the following components in weight percentage: phosphoserine 20%, taurine 20%, hydroxyproline 20%, γ - 20% aminobutyric acid and 20% carnosine are used as feeding ingredients in the fermentation production process, and are used in fed-batch feeding during the fermentation production process, and the fed-batch method is multiple intervals of input.

[0031] The dosages of phosphoserine, taurine, hydroxyproline, γ-aminobutyric acid and carnosine in the MRS basic fermentation medium are 5g-50g / ton respectively.

[0032] The application of an amino acid composition beneficial to the proliferation of Lactobacillus acidophilus in a fermentation process comprises the following application steps:

[0033] 1) Prepare the basic fermentation medium of Lactobacillus acidophilus MRS, weigh the components of the basic fermentation...

Embodiment 3

[0039] This example relates to an amino acid composition that is beneficial to the proliferation of Lactobacillus acidophilus. The amino acid composition includes the following components in weight percentage: 35% tyrosine, 30% cysteine, 25% gamma-aminobutyric acid % and 10% of δ-hydroxylysine are used as feeding ingredients in the fermentation production process, and are used in fed-batch feeding in the fermentation production process, and the fed-batch method is input at a constant speed.

[0040] The application of an amino acid composition beneficial to the proliferation of Lactobacillus acidophilus in a fermentation process comprises the following application steps:

[0041] 1) Prepare Lactobacillus acidophilus improved MRS basic fermentation medium, weigh the components of the basic fermentation medium according to the production scale of 5 tons: glucose 100kg, peptone 50kg, beef extract 50kg, yeast extract 25kg, sodium acetate 25kg, hydrogen citrate Diammonium 12.5kg, d...

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PUM

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Abstract

The invention discloses an amino acid composition beneficial to proliferation of lactobacillus acidophilus and an application thereof, which belong to the technical field of probiotic fermentation. The amino acid composition includes conventional amino acids and / or other amino acids. Other amino acids are the mixture of one or more of phosphoserine, phosphoethanolamine, ethanolamine, taurine, citrulline, homocitrulline , sarcosine, hydroxyproline, alpha-aminobutyric acid, alpha-aminoadipic acid, beta-alanine, beta-aminoisobutyric acid, ornithine, n-valine, alloisoleucine, n-isoleucine, homocysteine, delta-hydroxylysine, 1-methylhistidine, 3-methylhistidine, gamma-aminobutyric acid, carnosine, goose carnosine, cystathionine and arginine succinic acid. The content of viable bacteria in fermentation liquor is greater than 3.0*10<9> cfu / mL, and the content of viable bacteria in freeze-dried bacterial powder is greater than 3.5*10<11> cfu / g. The demand of the existing market for the numberof lactobacillus acidophilus is satisfied.

Description

technical field [0001] The invention belongs to the technical field of probiotic fermentation, and in particular relates to an amino acid composition beneficial to the proliferation of Lactobacillus acidophilus and an application thereof. Background technique [0002] Lactobacillus acidophilus belongs to the genus Lactobacillus. On the MRS agar solid plate, anaerobic culture at 37°C for 48 hours, a small, net-shaped, raised, rough surface, curly edge milky white colony is formed; it is a Gram-positive bacillus, and the end of the rod is round. [0003] Lactobacillus acidophilus can not only survive in the stomach, but the survival rate in the stomach can generally reach about 80%. It is also the main probiotic in the small intestine of the human body. Lactobacillus acidophilus exists in the small intestine and releases organic acids such as lactic acid and acetic acid, which can increase the utilization rate of calcium, phosphorus, and iron, promote the absorption of iron a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12R1/23
CPCC12N1/20C12P1/04C12R2001/23C12N1/205
Inventor 丁一宋士良代建胜丁力丁沛昊
Owner 郑州和合生物工程技术有限公司
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