Completely degradable tissue engineering skin scaffold material and preparation method thereof

Abstract

The invention discloses a completely degradable tissue engineering skin scaffold material which comprises two parts, namely, a bacterial cellulose / chitosan nanofiber layer and a large-aperture array micron fiber scaffold. The bacterial cellulose / chitosan nanofiber layer is an upper layer, the upper part of the large-aperture array micron fiber scaffold is a lower layer, and the layers are crosslinked and combined through glutaraldehyde. The bacterial cellulose / chitosan nanofiber layer is formed by in-situ synthesis of gluconacetobacter xylinum, and cellulase is fixed on the surface of the bacterial cellulose / chitosan nanofiber layer. The large-aperture array micron fiber scaffold is a scaffold formed by arranging electrostatic spinning lactic acid / hyaluronic acid / fibroin micro-nano fibersin an orderly manner, and the surface of the large-aperture array micron fiber scaffold is modified with polylactic acid-glycolic acid growth factor sustained-release microspheres. The used raw materials are non-toxic and harmless, the fiber property is stable, and good biocompatibility is achieved. The scaffold material can be completely degraded in vivo. The pore diameter of the lower-layer scaffold is close to the cell size, and cells can enter the scaffold. Growth factors can be stably released, and skin tissue regeneration is facilitated.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a fully degradable tissue engineering skin scaffold material and a preparation method thereof. Background technique [0002] As the largest organ of the human body, the skin plays a role in protecting the human body from the invasion of external objects. Damage to the skin can lead to the invasion of bacteria and pathogens, and severe cases can lead to death. Therefore, in the past three decades, a large number of studies on skin scaffolds have been carried out in the fields of biomedical materials and tissue engineering. Human skin is a double-layered structure consisting of epidermis and dermis. The epidermis is mainly composed of dense keratinocytes, which play a protective role; the dermis is mainly composed of fibroblasts and the collagen secreted by them, which has good elasticity and mechanical strength, and plays an important role in the skin regeneration p...

AI Technical Summary

Problems solved by technology

However, most of the nanofibrous membranes prepared by electrospinning cannot be completely degraded, and they are easy to shrink and deform, and the fiber stability is insufficient. When used as an epidermal scaffold for skin tissue engineering, it cannot meet the requirements of structural stability; the dermal scaffold structure has a relatively small pore size. Small, dense structure, not conducive to the problem of cells entering the scaffold to form a three-dimensional culture

When preparing scaffolds by 3D printing, although the porosity and pore size can be adjusted, which solves the problem of compact structure of electrospun fibers, since the printing materials need to reach a certain hardness to obtain the designed shape and structure, this results in the application of 3D printing scaffolds. In skin tissue engineering, the hardness is too high, it is difficult to completely degrade, it is not easy to adhere to the wound surface, and it is not conducive to cell adhesion and migration

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