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Method for extracting lemon phenol from Desmodium caudatum, and application thereof

An extraction method and a lemon phenol technology are applied in the field of extraction of lemon phenol from Sophora japonica, can solve the problems of incapable of industrialized production, complicated extraction process of lemon phenol, etc., and achieve the effects of being beneficial to industrial production, fewer steps and fewer consumables.

Inactive Publication Date: 2020-04-07
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for extracting lemon phenol from Sophora japonica, thereby overcoming the shortcomings of complex lemon phenol extraction process and incapable of industrial production

Method used

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  • Method for extracting lemon phenol from Desmodium caudatum, and application thereof
  • Method for extracting lemon phenol from Desmodium caudatum, and application thereof
  • Method for extracting lemon phenol from Desmodium caudatum, and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A method for extracting lemon phenol in Sophorae japonica, comprising the following steps:

[0038] (1) Raw material pretreatment: take the stems or / and leaves of Sophora japonica as raw materials, crush the natural dried Sophora japonica medicinal material through a pulverizer, and pass through a 60-mesh sieve to obtain a sample;

[0039] (2) Reflux: Put the weighed sample into the reflux device, add 9 times the sample volume of ethanol with a volume concentration of 50% and heat and reflux for extraction. The temperature of the ethanol heating and reflux extraction is 85°C, and the ethanol reflux time is 2h , to obtain the reflux extract,

[0040] (3) Concentration: filter the reflux extract, recycle the solvent by rotary evaporation, and concentrate to obtain a liquid extract;

[0041] (4) Sample preparation: adding the liquid extract to column chromatography silica gel and ethanol with a volume concentration of 50% to mix the sample, evaporated to dryness in a wate...

Embodiment 2

[0044] A method for extracting lemon phenol in Sophorae japonica, comprising the following steps:

[0045] (1) Raw material pretreatment: take the stems or / and leaves of Sophora japonica as raw materials, crush the natural dried Sophora japonica medicinal material through a pulverizer, and sieve to obtain samples;

[0046] (2) Reflux: Put the weighed sample into the reflux device, add 5 times the volume concentration of the sample volume and extract with 90% ethanol and heat it under reflux. Obtain the reflux extract;

[0047] (3) Concentration: filter the reflux extract, recycle the solvent by rotary evaporation, and concentrate to obtain a liquid extract;

[0048] (4) Sample preparation: add the liquid extract to column chromatography silica gel and ethanol with a volume concentration of 90% to mix the sample, evaporate to dryness in a water bath at 100° C. until a dispersed sample is obtained, and set aside; wherein, the amount of column chromatography silica gel added is ...

Embodiment 3

[0051] A method for extracting lemon phenol in Sophorae japonica, comprising the following steps:

[0052] (1) Raw material pretreatment: take the stems or / and leaves of Sophora japonica as raw materials, crush the natural dried Sophora japonica medicinal material through a pulverizer, and sieve to obtain samples;

[0053] (2) Reflux: Put the weighed sample into the reflux device, add 30% ethanol with a volume concentration of 20 times the sample volume, and heat and reflux for extraction. The temperature of the ethanol heating and reflux extraction is 90°C, and the ethanol reflux time is 3h , to obtain the reflux extract,

[0054] (3) Concentration: filter the reflux extract, recycle the solvent by rotary evaporation, and concentrate to obtain a liquid extract;

[0055] (4) Sample preparation: adding the liquid extract to column chromatography silica gel and ethanol with a volume concentration of 30% to mix the sample, evaporated to dryness in a water bath at 100°C, until a ...

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Abstract

The invention discloses a method for extracting lemon phenol from Desmodium caudatum. The method comprises the following steps: (1) pretreating raw materials; (2) carrying out reflux concentration; (3) preparing a sample: adding an obtained liquid extract into column chromatography silica gel and ethanol, stirring, and drying by distillation until a dispersed sample is obtained; and (4) carrying out column chromatography separation: putting the column chromatography silica gel into a chromatographic column, adding ethyl acetate to obtain a flowable solid-liquid mixture, adding the dispersed sample into the chromatographic column, eluting the chromatographic column with ethyl acetate, equivalently collecting all the fractions, then confirming whether lemon phenol is completely discharged ornot, enriching a part of fractions containing lemon phenol, and evaporating to obtain lemon phenol. The method for extracting lemon phenol from Desmodium caudatum is simple, easy to operate and few in step, and industrial production is facilitated. And the used solvents are few in variety and can be completely recycled. And the yield of lemon phenol in Desmodium caudatum is improved. The invention discloses application of lemon phenol in preparation of anti-liver cancer drugs, and applications in inhibition of proliferation and migration of human liver cancer HepG2 cells.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine preparations, in particular to a method for extracting lemon phenol from Sophorae japonica and its application. Background technique [0002] Desmodium caudatum (Thunb.) DC. is a plant of the genus Fabaceae and is a commonly used medicinal material for the Zhuang and Yao peoples in Guangxi. The Zhuang medicine is called Kewenzhan, and the Yao medicine is called Yamahong. Anhui, Zhejiang, Jiangxi and other provinces. Traditional Chinese medicine believes that it tastes sweet, bitter, and cool. It has the effects of clearing heat and detoxifying, expelling wind and rash, eliminating stagnation and relieving pain. Zhuang medicine believes that its taste is sweet, bitter, flat, adjusts the dragon road, ventilates the valley road, clears heat and poison, stops bleeding, and is used for heartache (stomach pain), white jelly (diarrhea), irregular menstruation, malnutrition (malnutrition), Po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61K31/352A61P35/00A61P35/04A61P1/16
CPCC07D493/04A61K31/352A61P35/00A61P35/04A61P1/16A61K36/48
Inventor 黄周锋胡筱希陆国寿黄建猷谭晓
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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