Method for visually detecting Cu < 2 + > based on GQDs photocatalysis

A photocatalytic, cu2 technology, applied in the field of chemical sensing, to achieve the effect of strong sensitivity, rapid detection and simple operation

Active Publication Date: 2020-03-24
FUQING BRANCH OF FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, compared with the fluorescence properties of GQDs, there are still no reports on the construction of sensors using the photocatalytic activity of GQDs.

Method used

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  • Method for visually detecting Cu &lt; 2 + &gt; based on GQDs photocatalysis
  • Method for visually detecting Cu &lt; 2 + &gt; based on GQDs photocatalysis
  • Method for visually detecting Cu &lt; 2 + &gt; based on GQDs photocatalysis

Examples

Experimental program
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Effect test

Embodiment 1

[0053] The preparation of embodiment 1GQDs

[0054] Take 0.200g graphene oxide (Graphene Oxide, GO), fully dissolve in 30mL concentrated H 2 SO 4 and 10mL HNO 3 In the mixed liquid, the mixed solution A was obtained; the mixed solution A was ultrasonically treated for 2h, and stirred at 100°C for 3h, and the product A was obtained after cooling to room temperature; the product A was diluted with ultrapure water and placed on ice Na in the bath 2 CO 3 Adjust the pH to 7 to obtain a mixed solution B; filter the mixed solution B through a 0.22 μm filter membrane to remove residual GO, and then obtain a mixed solution C; place the mixed solution C in a dialysis bag with a molecular weight cut-off of 100Da Dialyzed for 2 days to remove NaNO formed during neutralization 3 and Na 2 SO 4 , to obtain a mixed solution D; the mixed solution D was placed in a dialysis bag with a molecular weight cut-off of 1000 Da for further dialysis for another 2 days to obtain the product B in t...

Embodiment 2

[0056] Example 2 Detection of Cu based on GQDs photocatalytic activity 2+ Feasibility test of

[0057] 1. Study on the photooxidation mechanism of TMB

[0058] Related studies have shown that GQDs can generate reactive oxygen species (ROS) under light exposure. In the embodiment of the present invention, in order to reveal the role of special ROS in the oxidation of TMB (tetramethylbenzidine), a ROS-specific scavenger, namely mannitol of OH, was used. 1 o 2 of tryptophan, O 2 - superoxide dismutase (SOD) and H 2 o 2 of catalase. Such as Figure 2A As shown, only the presence of tryptophan has an inhibitory effect on GQD-catalyzed photooxidation of TMB, while the other three scavengers have no significant difference, indicating that the photosensitized 1 o 2 Generation is the cause of TMB oxidation.

[0059] generated as an authentication 1 o 2 The most direct evidence, choose 2,2,6,6-tetramethylpiperidine (TEMP) as capture agent, carry out electron spin resonance ...

Embodiment 3

[0064] The optimization of embodiment 3 sensing conditions

[0065] In order to obtain the best sensing performance, the embodiments of the present invention optimize the pH of the reaction buffer (citric acid buffer), the concentration of TMB, the incubation time and the irradiation time. Taking ΔA as the detection index, ΔA (ie, A 0 -A) Indicates in the absence and presence of Cu 2+ Absorbance changes in the case of oxTMB.

[0066] (1) This embodiment explores the influence of the reaction buffer solution with a pH value of 2.5 to 6.0 on the ΔA value, and the results are as follows Figure 4A shown. Depend on Figure 4A It can be seen that the photoactivity of GQD is highly dependent on pH, and a higher ΔA can be obtained at a pH of 3.5-4.5, and preferably, the ΔA value is the highest at a pH of 4.0.

[0067] (2) Further, this example explores the influence of TMB with a concentration of 0-1.0mM on the value of ΔA, and the results are as follows Figure 4B shown. Depe...

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Abstract

The invention belongs to the technical field of chemical sensing, and particularly relates to a method for visually detecting Cu < 2 + > based on GQDs photocatalysis. The invention provides a method for visually detecting Cu < 2 + > based on GQDs photocatalysis. The method comprises the following steps: preparing GQDs; preparing a sample solution; preparing standard solutions with different Cu < 2+ > concentrations; mixing to construct a sensing system; carrying out chromogenic reaction; carrying out colorimetric detection: determining the absorbance of the solution D at 650nm by using an ultraviolet spectrophotometer; making a standard curve; and determining the concentration of Cu < 2 + > in the to-be-detected water sample through the standard curve. In the method for visually detectingCu < 2 + > based on GQDs photocatalysis, a novel colorimetric platform is constructed by utilizing the photocatalytic activity of the GQDs, high sensitivity and selective sensing of Cu < 2 + > in anactual water environment can be realized, low-cost, rapid and on-site detection of a detection instrument is realized, and the method is simple to operate, economical, environment-friendly, rapid in detection, high in selectivity and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of chemical sensing, in particular to a GQDs photocatalytic visual detection of Cu 2+ Methods. Background technique [0002] Copper(II) is an essential trace element that plays important roles in the environmental, biological and chemical fields. Copper deficiency in humans can lead to blood and nervous system disorders, and copper deficiency in plants can affect photosynthesis, respiration, reproduction, protein formation, lignification, auxin regulation, disease resistance, and certain other plant functions. However, high concentrations of copper ions (Cu 2+ ) cause toxic effects in living organisms and are associated with many diseases such as anemia cancer, cardiovascular disease, Wilson's disease and other neurodegenerative diseases. In addition, Cu 2+ It is widely used in industry and agriculture, so it is also a serious environmental pollutant. In view of this, the U.S. Environmental Protection A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/10G01N21/33G01N21/64
CPCG01N31/10G01N21/33G01N21/6402
Inventor 陈志涛刘晶晶
Owner FUQING BRANCH OF FUJIAN NORMAL UNIV
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