Galactooligosaccharides and their derivatives and their application as drugs or health products for improving mitochondrial function and preventing insulin resistance-related diseases

A technology of galactooligosaccharides and derivatives, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve problems such as potential safety hazards, improve oxidative stress status, facilitate industrialization, and simple preparation process Effect

Active Publication Date: 2021-11-12
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although carrageenan and its oligosaccharides have antiviral activity as a spray (public number CN102516323A, public number CN104546895A), oral administration has certain safety hazards (Shang Q., Toxicol Let., 2017, 279:87-95), Agar gum and laver gum polysaccharides and oligosaccharides are very safe when taken orally, and are high-quality raw materials for the development of drugs and functional foods

Method used

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  • Galactooligosaccharides and their derivatives and their application as drugs or health products for improving mitochondrial function and preventing insulin resistance-related diseases
  • Galactooligosaccharides and their derivatives and their application as drugs or health products for improving mitochondrial function and preventing insulin resistance-related diseases
  • Galactooligosaccharides and their derivatives and their application as drugs or health products for improving mitochondrial function and preventing insulin resistance-related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Containing 6-O-sulfate-β-1,3-D-galactose (Gal6S) and α-1,4-L-3,6-inner ether galactose (AnG) thiogaran oligosaccharide ( Preparation of SAOs), oligosaccharide alcohols (SAOs-OH) and oligosaccharide acids (SAOs-OOH).

[0034] Prepare 1000mg of sulfur agar polysaccharide with dilute sulfuric acid with a molar concentration of 0.1M to form a 10mg / mL aqueous solution, heat it to 60°C, stir and degrade it for 1.5 hours, neutralize it with an aqueous NaOH solution with a molar concentration of 2M after cooling, collect the supernatant by centrifugation, and then Add 3 times the volume of 95% medical ethanol at 4°C overnight, centrifuge to collect the supernatant, remove the ethanol by rotary evaporation under reduced pressure, use a 200Da dialysis bag for dialysis desalination, concentrate by rotary evaporation, and freeze-dry to obtain SAOs. Take 100 mg of SAOs and dissolve it in 10 mL of 100 mM NaBH 4 The aqueous solution (containing NaOH with a molar concentrat...

Embodiment 2

[0039] Example 2: Laver gum oligosaccharides (POs), oligosaccharide alcohols (POs) containing β-1,3-D-galactose (Gal) and 6-O-sulfate-α-1,4-galactose (Gal6S) -OH) and the preparation of oligosaccharide acids (POs-OOH).

[0040]Prepare laver gum with dilute sulfuric acid with a molar concentration of 0.1M to prepare a 10mg / mL aqueous solution, heat it to 80°C, stir and degrade it for 2.0 hours, neutralize it with an aqueous NaOH solution with a molar concentration of 2M after cooling, collect the supernatant by centrifugation, and add 4 times the volume 95% medical ethanol was placed overnight at 4°C, the supernatant was collected by centrifugation, and the ethanol was removed by rotary evaporation under reduced pressure, then desalted with a 200Da dialysis bag, concentrated by rotary evaporation, and freeze-dried to obtain laver gum POs. Take 150 mg of POs oligosaccharides and dissolve it in 15 ml of NaBH with a molar concentration of 150 mM 4 The aqueous solution (containing...

Embodiment 3

[0045] Example 3: Agar oligosaccharides containing β-1,3-D-galactose (Gal) and α-1,4-L-3,6-galactose (AnG) and their oligosaccharide alcohols and oligosaccharides acid preparation.

[0046] Dissolve the agarose in hot water, use dilute hydrochloric acid with a molar concentration of 0.1M to prepare a 10mg / mL solution, stir and degrade it at 80°C for 0.5 hours, neutralize it with an aqueous NaOH solution with a molar concentration of 2M after cooling, collect the supernatant by centrifugation, and then Add 2.5 times the volume of 95% medical ethanol at 4°C overnight, centrifuge to collect the supernatant, remove the ethanol by rotary evaporation, dialyze and desalt with a 200Da dialysis bag, concentrate by rotary evaporation and freeze-dry to obtain agar oligosaccharide AOs, and further reduce it with sodium borohydride to obtain agar Glucooligosaccharide alcohol AOs-OH, or agarooligosaccharide acid AOs-OOH obtained by oxidation with Benedict's reagent. The chemical structural...

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Abstract

The invention belongs to the field of marine medicines, and in particular relates to an oligosaccharide containing D-galactose and L-galactose and its derivatives and its application as a drug or health product for improving mitochondrial function and preventing insulin resistance-related diseases. Using red algal polysaccharides containing D‑ / L‑galactose as raw materials, degrade them by physical methods, chemical methods, biological enzyme methods or any combination of the above methods to prepare galactooligosaccharides and their derivatives with different degrees of polymerization, Its molecular skeleton contains D‑galactose and L‑galactose and their derivatives. The raw material of the product of the present invention is derived from red algae polysaccharide, which has the advantages of rich resources, simple preparation process, high safety, clear target and easy industrialization. The development field of special medical food such as blood fat and blood pressure lowering has broad application prospects.

Description

technical field [0001] The invention belongs to the field of marine medicines, and in particular relates to an oligosaccharide containing D-galactose and L-galactose and its derivatives and its application as a medicine or health product for improving mitochondrial function and preventing insulin resistance-related diseases. Background technique [0002] Studies have found that long-term abnormal blood sugar, blood lipids, and blood pressure will cause damage to the organs of the whole body and lead to their function decline, eventually leading to diseases such as hyperglycemia, hyperlipidemia, and hypertension. Mitochondria are important energy metabolism organelles for cell life activities. Mitochondrial dysfunction will lead to the occurrence and development of various metabolic diseases. For example, Shulman proposed that mitochondrial dysfunction is an important mechanism of insulin resistance. This view has obtained many experimental results support. Mitochondrial dys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08B37/12C07H11/00C07H15/04C07H3/06C07H1/00A61P5/48A61P3/10A61P3/00A61P3/06A61P1/16C12P19/14C12P19/12C12P19/04C12P19/00
CPCA61P1/16A61P3/00A61P3/06A61P3/10A61P5/48C07H1/00C07H3/06C07H11/00C07H15/04C08B37/0036C08B37/0039C12P19/00C12P19/04C12P19/12C12P19/14
Inventor 于广利王学良郝杰杰蔡超
Owner OCEAN UNIV OF CHINA
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