Morchella esculenta mother strain culture medium and morchella esculenta mother strain preparation method

The technology of morel and culture medium is applied in the field of the culture medium of mother hickory chick and the preparation field of hickory hickory mother species, which can solve the inconvenience, the inability to store potatoes for a long time, and the mycelium growth speed of hickory chick mother species Slow and other problems, to achieve the effect of low cost, short full tube time, and convenient source

Inactive Publication Date: 2019-12-31
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of them use PDA medium, i.e. potato dextrose agar medium. On the one hand, the growth rate of the morel mother species hyphae prepared by PDA medium is relatively slow; on the other hand, because potatoes cannot be stored for a long time, each experiment needs to It is inconvenient to buy new potatoes

Method used

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  • Morchella esculenta mother strain culture medium and morchella esculenta mother strain preparation method
  • Morchella esculenta mother strain culture medium and morchella esculenta mother strain preparation method
  • Morchella esculenta mother strain culture medium and morchella esculenta mother strain preparation method

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preparation example Construction

[0022] Preparation of morel mother species:

[0023] (1) Juicing: Weigh each substance according to the mass percentage, take the potato, peel and remove the bud eyes, cut into 1m 3 Small pieces, add small potato pieces into water, boil on high heat, then switch to low heat and continue to boil for about 20 minutes, filter to get juice;

[0024] (2) Culture medium preparation: make up the lost water in the filtered juice, add agar, simmer to dissolve, then add glucose, stir until dissolved, and the pH is natural;

[0025] (3) Subpackage: Subpackage the culture medium in the Erlenmeyer flask, the subpackage of the culture medium is 2 / 3 of the capacity of the Erlenmeyer flask, seal it with a silica gel plug, and wrap the bottle mouth with kraft paper;

[0026] (4) Sterilization: subject the wrapped triangular flask and packaged clean petri dish to high-pressure steam sterilization, and sterilize at 121° C. for 30 minutes;

[0027] (5) Pour plate: under aseptic conditions, pour...

Embodiment 1

[0100] Culture medium: 2% glucose, 5% rice, 2% agar, 0.1% calcium sulfate, the balance is water, and the pH value is natural.

[0101] Preparation of morel mother species:

[0102] (1) Juicing: put 50g of rice into a small pot, add 900mL of water, bring to a boil on high heat, then switch to a slow fire and continue to boil for 20 minutes, filter to get the juice;

[0103] (2) Culture medium preparation: add 20g of agar to the filtrate to make up for the water loss, simmer to dissolve, then add 20g of glucose and 1g of calcium sulfate, stir until dissolved, set the volume to 1L, and the pH is natural;

[0104] (3) Subpackage: subpackage the medium into test tubes, the subpackage of the medium is 1 / 5 of the length of the test tube, and seal it with a silica gel plug;

[0105] (4) Bundling: get 7 test tubes with culture medium and bundle them together, and wrap the test tube mouth with double-layer newspaper;

[0106] (5) Sterilization: subject the bundled test tubes to high-p...

Embodiment 2

[0112] Medium: 2% glucose, 5% rice, 2% agar, 0.1% calcium sulfate, the balance is water, pH 8.

[0113] Preparation of morel mother species:

[0114] (1) Juicing: Put 50g of rice into a small pot, add 900mL of water, bring to a boil on high heat, then switch to a slow fire and continue to boil for 15 minutes, filter to get the juice;

[0115] (2) Culture medium preparation: add 20g of agar to the filtrate to make up for water loss, simmer to dissolve, then add 20g of glucose and 1g of calcium sulfate, stir until dissolved, set the volume to 1L, and adjust the pH to 8;

[0116] (3) Subpackage: subpackage the medium into test tubes, the subpackage of the medium is 1 / 4 of the length of the test tube, and seal it with a silica gel plug;

[0117] (4) Bundling: Get 7 test tubes with culture medium and bundle them together, and wrap the test tube mouth with kraft paper;

[0118] (5) Sterilization: subject the bundled test tubes to high-pressure steam sterilization, and sterilize at...

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Abstract

The invention discloses a morchella esculenta mother strain culture medium and a method for preparing a morchella esculenta mother strain by the morchella esculenta mother strain culture medium. The culture medium is prepared from, by mass, 1%-5% of one of glucose, saccharose or lactose, 3%-8% of one of rice, wheat or corn flour, 1.5%-2% of agar, one of 0%-0.5% of magnesium sulfate or calcium sulfate and the balance water. Material sources of the culture medium are convenient, formula simplicity and low cost are realized, and by replacement of potatoes in a PDA culture medium with rice, the problem that potatoes which cannot be stored for a long time need to be bought again in each experiment; by additional adding of calcium sulfate in the culture medium, hypha growth can be effectively promoted; by an orthogonal experiment for determining optimal components and contents of the culture medium, a prepared morchella esculenta mother strain is dense in hypha growth, robust and high in growing speed and growth vigor, full-tube time is short and only needs 2-3 days, and accordingly large-scale popularization and application can be carried out.

Description

technical field [0001] The invention relates to a culture medium for female species of edible fungi and a preparation method for the female species of edible fungi, in particular to a culture medium for female species of morels and a method for preparing female species of morels using the medium. Background technique [0002] Morel (Morchella esculenta (L.) Pars.), commonly known as Morchella esculenta, net bag mushroom, is named for its uneven cap surface, which presents a wrinkled net shape and is shaped like a Morchella. It is a rare food and medicine With fungus. Morchella is delicious, nutritious, and has high medicinal value, especially the amino acid content is the first of all edible fungi. Ancient books record that Morchella is flat in nature, sweet and cold in taste, and non-toxic. Modern pharmacological research has found that Morchella can regulate immune function, lower blood lipids, resist fatigue, resist radiation, and resist tumors. . [0003] Because hic...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40
CPCA01G18/20A01G18/40
Inventor 凡军民谢春芹邢忍忍张雪松许俊齐
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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