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Application of sialic acid in the preparation of protective drugs for intestinal tract or dna damage caused by ionizing radiation

A technology of ionizing radiation and sialic acid, which is used in drug combinations, pharmaceutical formulations, and medical preparations containing active ingredients. , the effect of convenient medication

Active Publication Date: 2022-04-05
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on protective drugs for intestinal damage caused by ionizing radiation is mostly in the preclinical stage. Although some drugs have protective effects, they have significant shortcomings, mainly including: high concentration, low activity, and obvious toxic and side effects, which limit clinical application. Finding non-toxic or low-toxic, highly effective protective drugs for intestinal damage caused by ionizing radiation has always been a hot pursuit in the medical field

Method used

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  • Application of sialic acid in the preparation of protective drugs for intestinal tract or dna damage caused by ionizing radiation
  • Application of sialic acid in the preparation of protective drugs for intestinal tract or dna damage caused by ionizing radiation
  • Application of sialic acid in the preparation of protective drugs for intestinal tract or dna damage caused by ionizing radiation

Examples

Experimental program
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Effect test

Embodiment 1

[0034] First, a mouse model of intestinal injury induced by radiation (X-rays) was established. C57BL / 6 mice aged 6-8 weeks, half male and half male (body weight 25±2g), were randomly divided into two groups: irradiation group (10Gy) with 15 mice and The irradiation combined with sialic acid administration group (10Gy+sialic acid) consisted of 15 mice; the whole body of the mice was uniformly irradiated with a single X-ray irradiation machine, and the absorbed dose of the mice was 10Gy. Dissolve sialic acid in 0.01M phosphate aqueous solution to obtain a mixed solution, wherein the concentration of sialic acid in the mixed solution is 5 mg / mL, and the mice in the irradiation combined with sialic acid administration group are administered intragastrically (dose: 50 mg / kg) before irradiation Irradiation was carried out 1 hour later, and the mice in the irradiation group were given phosphate aqueous solution of the same volume and mass concentration. One day, two days and three d...

Embodiment 2

[0036] (1) Firstly, an X-ray-induced mouse intestinal injury model was established. Select 6-8 weeks old C57BL / 6 mice, half male and half male (body weight 25±2g), and randomly divide them into four groups: 6 mice in the control group without irradiation treatment and normal feeding, 6 mice in the simple sialic acid treatment group, and 6 mice in the irradiation group. 6 rats, 6 rats in the sialic acid treatment group before and after irradiation (three times). The mice were uniformly irradiated in a single whole body with an X-ray machine, and the absorbed dose of the mice was 10Gy. Sialic acid was dissolved in 0.01M phosphate aqueous solution, and the concentration of sialic acid was 5 mg / mL. Before irradiation, the mice in the sialic acid treatment group were intragastrically administered (dose: 50 mg / kg) for 1 hour before irradiation, and the mice in the irradiation group were given Phosphate aqueous solution with the same volume and mass concentration. One day, two days...

Embodiment 3

[0042] Human intestinal epithelial cells HIEC, which are more recognized in the research of intestinal injury in vitro, were selected as the model. Dissolve sialic acid in phosphate buffer solution, the concentration of the mother solution is 20 mg / mL, and prepare and use sialic acid solutions with concentrations of 50 and 100 μg / mL respectively by the method of serial dilution, and the diluent is DMEM medium for culturing cells. For the control group, an equal volume of 100 μg / mL sialic acid solution in phosphate buffer was used. HIEC cells were seeded in 6-well plates. When the cells were in the logarithmic growth phase, the above-mentioned different concentrations of bird's nest acid solutions and phosphate buffer saline were added as controls. After 2 hours of treatment, the cells were irradiated with 2Gy X-rays. After irradiation, 0, 1, 4 and 12 hours the cells were fixed with 4% paraformaldehyde, and the fixed cells were immunofluorescence stained with an antibody agains...

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Abstract

The invention relates to the application of sialic acid in the preparation of medicines for protecting intestinal tract or DNA damage caused by ionizing radiation, and discloses the dosage forms and administration methods. The protective drug for intestinal damage caused by ionizing radiation with sialic acid as the active ingredient provided by the present invention has the advantages of small toxic and side effects, significant curative effect, convenient and safe medication, etc. It is applicable to different personnel such as patients, and can be used before treatment or during tasks and work. At the same time, the invention provides a new application, treatment concentration and treatment mode of sialic acid as a DNA damage repair protein DNA-PKcs activator, and has broad potential application prospects in the field of biomedicine.

Description

technical field [0001] The invention relates to the field of drugs related to the treatment of radiation-induced intestinal damage, in particular to the application of sialic acid in the preparation of drugs for protecting the intestinal tract or DNA damage caused by ionizing radiation. Background technique [0002] The small intestine is very sensitive to ionizing radiation due to the rapid renewal of epithelial cells in the small intestine, and is the most important target organ of ionizing radiation. Intestinal damage caused by ionizing radiation is one of the important causes of early death in patients with acute radiation sickness. In addition, patients with malignant tumors are another special population exposed to ionizing radiation. Radiation therapy is an important treatment for malignant tumors. According to reports, more than 70% of patients with malignant tumors need radiation therapy. The common side effects of treatment lead to a significant reduction in the qu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7012A61P1/00
CPCA61K31/7012A61P1/00
Inventor 尚增甫郎月周平坤周俊东俞岚
Owner SUZHOU UNIV
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