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Lactobacillus plantarum with function of reducing blood lipids and application of lactobacillus plantarum

A technology of Lactobacillus plantarum and blood lipids, which is applied in the directions of lactobacillus, medical preparations containing active ingredients, applications, etc., can solve the problems such as the incomplete blood lipid lowering function of lactic acid bacteria, achieve good acid and bile salt tolerance, reduce blood lipids Effect

Inactive Publication Date: 2019-11-22
诺佰克(武汉)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the lactic acid bacteria disclosed in the prior art have incomplete functions for lowering blood lipids, and especially lack a kind of lactic acid bacteria that can not only reduce serum cholesterol, triglycerides and low-density lipoproteins, but also effectively reduce the contents of high-density lipoproteins, alanine aminotransferase and aspartate aminotransferase. Lactic acid bacteria

Method used

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  • Lactobacillus plantarum with function of reducing blood lipids and application of lactobacillus plantarum
  • Lactobacillus plantarum with function of reducing blood lipids and application of lactobacillus plantarum
  • Lactobacillus plantarum with function of reducing blood lipids and application of lactobacillus plantarum

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preparation example Construction

[0028] The present invention also provides a preparation method for the preparation described in the above scheme, comprising the following steps:

[0029] 1) inoculating Lactobacillus plantarum nbk-MA2 into the seed medium, activating it, and obtaining the primary seed liquid;

[0030] 2) inoculating the primary seed liquid described in step 1) into the seed medium, and culturing to obtain the secondary seed liquid;

[0031] 3) inoculating the secondary seed liquid in step 2) into the fermentation medium, carrying out fermentation, and centrifuging the fermentation liquid to obtain thallus;

[0032] 4) Drying and pulverizing the cells described in step 3) to obtain a preparation.

[0033] In the present invention, Lactobacillus plantarum nbk-MA2 is first inoculated into the seed medium and activated to obtain a first-grade seed liquid; the present invention has no special limitation on the inoculation amount of the Lactobacillus plantarum; the seed medium preferably includes...

Embodiment 1

[0045] Screening and identification of embodiment 1 Lactobacillus plantarum nbk-MA2

[0046] Tibetan kefir grains were collected from the homes of local residents in Tibet, ground with sterile saline and diluted to an appropriate gradient (10 2 ~10 5 ), were streaked on the MRS agar plate using the four-section line method, and cultured anaerobically in a 37°C incubator for 48h. Select a single colony, microscopically examine, streak, and repeat several times of isolation and purification to obtain Lactobacillus plantarum nbk-MA2. It was identified as Lactobacillus plantarum by molecular biology and named as Lactobacillus plantarum nbk-MA2.

Embodiment 2

[0047] Embodiment 2 A kind of preparation method comprising the bacterial powder of plantaractobacillus nbk-MA2 live bacteria

[0048] In terms of mass percentage, seed medium: 1.5% glucose, 1.8% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.4% ammonium citrate, 0.02% magnesium sulfate, 0.06% manganese sulfate, 0.1% Tween 80 and the balance of water, pH 6.5;

[0049] By mass percentage, fermentation medium: 2% glucose, 1.3% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.2% ammonium citrate, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.1% Tween 80 and the balance of water, the pH value is 6.4;

[0050] In parts by mass, the lyoprotectant: 2 parts of 10% skim milk, 2 parts of 3% glycerin and 5 parts of water.

[0051] Preparation

[0052] 1) Inoculate Lactobacillus plantarum into a test tube containing seed medium for activation (37°C, 20h) to obtain a first-grade seed liquid;

[0053] 2) Transfer the primary seed solution to the seed me...

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Abstract

The invention provides lactobacillus plantarum with a function of reducing blood lipids and application of the lactobacillus plantarum, and belongs to the technical field of new bacterial strains of lactobacillus and application of the new bacterial strains. According to the lactobacillus plantarum nbk-MA2, the preservation number is CGMCC NO.16872. Bacterial powder and inactivated bacterial powder of the lactobacillus plantarum nbk-MA2 can reduce the content of triglyceride, total cholesterol, glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, high-density lipoprotein and low-density lipoprotein in a body, recovering to the normal level is achieved, and the effect of reducing blood lipids is achieved; in addition, the lactobacillus plantarum nbk-MA2 has good acid and bile salt tolerance, and enough quantity of living bacteria can reach the intestinal tract and play a probiotic role.

Description

technical field [0001] The invention relates to the technical field of new bacterial strains of the genus Lactobacillus and its application, in particular to a strain of Lactobacillus plantarum with the function of lowering blood fat and its application. Background technique [0002] Dyslipidemia is one of the important risk factors for cardiovascular disease in my country. Blood lipids are the general term for cholesterol, triglycerides, and lipids (such as phospholipids) in serum. In the past ten years, the number of patients with dyslipidemia in my country has increased significantly. With the improvement of national living standards, dietary structure has changed. Hyperlipidemia is no longer exclusive to the elderly. Statistics show that the population of patients with hyperlipidemia is developing towards younger age. . Dyslipidemia, especially elevated low-density lipoprotein (LDL-C) is a key factor leading to the occurrence and development of atherosclerotic cardiovas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/16A61K35/747A61P3/06C12R1/25
CPCC12N1/20A23L33/135A23K10/16A61P3/06A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/30A23V2200/3262A23V2200/3204
Inventor 张阳潘芬湛剑龙
Owner 诺佰克(武汉)生物科技有限公司
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