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PVP-modified sodium alginate/polydopamine composite nano-material and preparation and application thereof

A technology of composite nanomaterials and sodium alginate, which is applied in the direction of medical preparations with non-active ingredients, wave energy or particle radiation treatment materials, medical preparations containing active ingredients, etc., can solve complex synthesis processes, low photothermal efficiency, Limiting the application of tumor therapy and other issues, to achieve the effects of broad application prospects, excellent photothermal transfer efficiency, controlled drug release ability, and excellent in vivo/in vitro biocompatibility

Active Publication Date: 2019-11-05
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, organic photothermal materials also have some serious problems, such as complex synthesis process and low photothermal efficiency, which largely limit their application in tumor therapy.

Method used

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  • PVP-modified sodium alginate/polydopamine composite nano-material and preparation and application thereof
  • PVP-modified sodium alginate/polydopamine composite nano-material and preparation and application thereof
  • PVP-modified sodium alginate/polydopamine composite nano-material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of sodium alginate / polydopamine composite nanomaterial modified by PVP, the specific steps are:

[0033] Step 1: 0.2g sodium alginate is dissolved in distilled water (10mL), prepares 2% sodium alginate solution, then, dopamine hydrochloride is dissolved in the above-mentioned solution, stir constantly, obtain the aqueous solution containing sodium alginate and dopamine ( Weight ratio ALG:DA=1:1).

[0034] Step 2: In CaCl 2 2H 2 Tris (Tris) was added to the aqueous solution of O and PVP to adjust the pH=10 to obtain a coagulation bath, wherein, Ca 2+ The ion concentration was 2%, the PVP concentration was 1%, and the Tris concentration was 0.5%.

[0035] Step 3: Using the electrostatic spraying method, spray the aqueous solution containing sodium alginate and dopamine into the coagulation bath at a constant speed using a push injection device, and form a jet under the action of electrostatic force and surface tension. The entire electrostatic sprayi...

Embodiment 2

[0037] The morphology and size of the ALG / PDA-PVP nanogel obtained in Example 1 were observed and measured by a scanning electron microscope. The hydrodynamic size of the particles and their stability are measured by dynamic light scattering. Using FTIR to analyze and measure the structure of ALG / PDA-PVP nanogel, through 4000~400cm -1 The transmission mode, collects data at a speed of 32 scans / min. Depend on figure 1 (a, b) It can be seen that the material synthesized in Example 1 has a spherical particle structure.

[0038] analyze figure 1 (c) It can be seen that the infrared spectrum of the ALG / PDA-PVP composite nanomaterial shows that the material is at 3438cm -1 , 1649cm -1 , 1519cm -1 , 1290cm -1 and 1056cm -1 There is an absorption peak. Of which 3500cm -1 The left and right are O-H or N-H stretching vibrations shared by ALG, PDA and PVP. 1650cm -1 The left and right are the C=O stretching vibrations on both ALG and PVP, and the 1520cm -1 The left and right...

Embodiment 3

[0040] Using a preparation method similar to that in Example 1, the difference is that step 1 also includes: dissolving DOX in an aqueous solution containing sodium alginate and dopamine (the final concentration of DOX is 1 mg / mL); the seaweed modified by PVP is obtained in step 3 After sodium alginate / polydopamine (ALG / PDA) composite nanogel, 2 mL of the prepared sample was centrifuged (10,000 rpm, 10 min), the gel was collected and washed three times with distilled water, and the resulting PVP-modified sodium alginate / polydopamine was collected. (ALG / PDA) composite nanogel and supernatant, and refrigerate the gel and supernatant for later use.

[0041] First use the ultraviolet-visible spectrophotometer to measure and draw the standard curve of DOX, and then measure the absorbance of the supernatant at a wavelength of 480nm. According to Lambert-Beer’s law, the concentration of DOX in the supernatant can be determined, thereby calculating the load efficiency. The steps of d...

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Abstract

The invention provides a preparation method of a PVP-modified sodium alginate / polydopamine composite nano-material. The preparation method is characterized by comprising the following steps: sprayingan aqueous solution containing sodium alginate and dopamine to a coagulating bath with pH 9-11 and containing polyvinylpyrrolidone (PVP), CaCl2 and trihydroxymethyl aminomethane, centrifuging and separating, and washing to obtain the PVP-modified sodium alginate / polydopamine composite nano-material. In vitro / in vivo biosecurity experiments and cancer therapy experiments prove that the PVP-modifiedsodium alginate / polydopamine composite nano-material has excellent biocompatibility, phototherapeutic ability and photothermal therapy and chemotherapy combined treatment ability.

Description

technical field [0001] The invention belongs to the field of bionano materials, and relates to a preparation method of a composite photothermal material ALG / PDA-PVP and research on tumor treatment thereof. Background technique [0002] Cancer endangers the lives of countless people, and traditional treatment methods such as surgical resection and chemotherapy have obvious defects such as damage to normal tissues and drug resistance to chemotherapy drugs. Photothermal therapy (PTT) is the use of materials with near-infrared absorption to convert light energy into heat energy to ablate cancer cells and kill cells. Compared with traditional cancer treatment, photothermal therapy (PTT) has the advantages of low toxicity, non-invasive (non-invasive), specific treatment, especially avoiding the drug resistance of chemotherapy. In recent decades, more and more researchers have devoted themselves to finding suitable photothermal conversion agents with high photothermal conversion e...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/704A61K47/34A61K47/36A61K47/59A61P35/00D01D5/00
CPCA61K31/704A61K41/0052A61K41/0057A61K47/59A61K47/36A61K47/34A61P35/00D01D5/0046D01D5/0092A61K2300/00
Inventor 王世革徐杨杰
Owner UNIV OF SHANGHAI FOR SCI & TECH
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