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Bio-organic fertilizer applicable to rape and application

A bio-organic fertilizer and organic fertilizer technology, applied in the direction of organic fertilizer, organic fertilizer preparation, bio-organic part treatment, etc., can solve the problem of incomplete killing of harmful bacteria and insect eggs, safety risk of infecting crops, and low conversion rate of organic matter and other problems, to achieve the effect of inhibiting Sclerotinia sclerotiorum, improving crop quality, and promoting crop growth

Active Publication Date: 2019-11-01
湖北新保得生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing organic fertilizer bio-fermentation technology has the following disadvantages: the raw materials of livestock and poultry manure are widely used, the fermentation cycle is long, the killing of harmful bacteria and insect eggs is not complete, the conversion rate of organic matter is low, and the deodorization effect is poor, which may There are heavy metals or drug residues, which have certain safety hazards
Rapeseed meal and rapeseed are both derived from rapeseed crops, which meet most of the nutrient requirements for the growth of rapeseed crops, but direct application has potential safety risks of plant pathogenic microorganisms infecting crops

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Mutation screening of Aspergillus oryzae protease high-producing strains:

[0037] Cobalt 60 mutagenesis was carried out in the Irradiation Center of Hubei Academy of Agricultural Sciences. Get the fresh, spore-rich Aspergillus oryzae bacterial strain slant, wash the spores with an appropriate amount of sterile water containing 0.1% Tween 80, count the hemocytometer, and dilute the plate to ensure that the number of spores is 10 7 individual / mL or more. Take 5ml of spore suspension and place them in 4 sterile Erlenmeyer flasks, and receive cobalt-60 gamma ray irradiation at doses of 185Gy, 395Gy, 605Gy, and 815Gy respectively, while the control does not receive irradiation. Each spore liquid after receiving radiation irradiation was spread on the casein medium, cultivated, counted and selected 70-80 strains of colonies with large transparent circles, and preserved them on the PDA slant.

[0038] The strains that were screened and preserved were cultured in bran medium...

Embodiment 2

[0045] Screening of Lactobacillus plantarum L16 strain:

[0046] 1. Preparation of culture medium:

[0047] BCP medium: peptone 5g; yeast extract 3g; lactose 5g; agar 20g; 0.5% bromocresol violet 10ml; distilled water 1000ml; pH 6.8-7.0.

[0048] MRS liquid medium: glucose 20g / L; peptone 10g / L; yeast extract 5g / L; beef extract powder 10g / L; diammonium citrate 2g / L; sodium acetate 5g / L; Tween 80 1g / L; K2HPO4 2g / L; MgSO4·7H2O 0.058g / L; MnSO4·4H2O 0.25g / L; distilled water 1000ml; pH6.5-6.8.

[0049] PDA medium: potato 200g; glucose 20g; agar 15-20g; distilled water 1000ml; natural pH.

[0050] For solid medium, 1.5% agar was added on the basis of liquid medium, and all medium was sterilized at 115°C for 20 minutes.

[0051] 2. Preliminary screening: Take pickles, orchard rhizosphere soil, rotten leaves of vegetable field and chopped pepper samples 5g each, put them in 300ml MLS liquid medium for 12 hours, then take 1ml of culture medium for gradient dilution, spread on BCP pla...

Embodiment 3

[0060] Inhibitory effect of Lactobacillus plantarum L16 on pathogenic microorganisms in rapeseed:

[0061] Use a hole puncher with a diameter of 0.6 cm to punch out the cultured rapeseed pathogenic bacteria sheet on the PDA plate, transfer it to the center of another PDA plate, and inoculate the activated Lactobacillus plantarum L16 symmetrically at a distance of 3 cm from the edge of the pathogenic bacteria colony with a pick needle , the control was only inoculated with pathogenic bacteria, and then cultured at a constant temperature of 26°C. When the control is full of plates, measure the average colony diameter of pathogenic bacteria and the average bacteriostatic bandwidth of lactic acid bacteria, calculate the bacteriostatic rate, and measure the strength of antagonism with the average bacteriostatic rate size and average bacteriostatic bandwidth of lactic acid bacteria.

[0062] Strain L16 showed good antagonistic effect on two kinds of pathogenic bacteria in rapeseed, ...

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Abstract

The invention belongs to the field of bio-organic fertilizers and discloses a bio-organic fertilizer applicable to rape and an application. The bio-organic fertilizer comprises the following raw materials in percentage by weight: 45% to 55% of grain stillage powder, 20% to 25% of rape seed meal, 15% to 25% of rape stalk powder, 0.3% to 0.5% of composite microbial agent and 5% to 8% of Procambarusclarkii shell meal. The bio-organic fertilizer disclosed by the invention is obtained through carrying out fermentation by the specific composite microbial agent. According to the bio-organic fertilizer applicable to the rape and the application, agricultural wastes such as grain stillage, rape stalks and Procambarus clarkii shells can be effectively utilized, the production cost can be reduced, the incidence rate of sclerotinia rot of rape can be lowered, and the yield of the rape can be increased.

Description

technical field [0001] The invention belongs to the field of bio-organic fertilizers, and specifically discloses a bio-organic fertilizer suitable for rapeseed and its application. Background technique [0002] As an important material input in modern agricultural production, fertilizers play an extremely important and irreplaceable role in ensuring the high and stable yield and quality potential of crops. The planting area of ​​rapeseed in my country is over 100 million mu. Compared with other major rapeseed producing countries in the world, the overall site conditions for rapeseed planting in my country are poor, the soil organic matter content is low, the types and degrees of nutrient deficiency are high, and the soil fertilizer retention capacity is poor, resulting in poor soil foundation. The low level of soil fertility, coupled with the fact that most rapeseed is planted as a winter crop in my country, low temperature, excessive soil moisture and insufficient moisture, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05F17/00
CPCC05F1/005C05F17/00C05F5/002C05F5/008C05F11/00C05F11/08Y02W30/40
Inventor 马丽曹立湘赵述淼赵星曹伟
Owner 湖北新保得生物科技有限公司
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