A preparation method of low molecular weight hyaluronic acid based on near-infrared spectroscopy

Abstract

The invention provides a preparation method of low molecular weight hyaluronic acid based on the near-infrared spectroscopy (NIRS). The real-time monitoring of HA enzymatic hydrolysis process is realized by using the NIRS technology; and through a constructed NIRS-based LMW-HA Mr prediction model, LMW-HA Mr information is acquired in real time, and then LMW-HA having a specific molecular weight isprepared. By the method, the enzymatic hydrolysis situation can be timely mastered, on-line real-time monitoring of HA Mr is realized, and adjustments can be made according to the current situation at any time so as to better achieve the purpose of producing the specific LMW-HA.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for preparing low-molecular-weight hyaluronic acid based on near-infrared spectroscopy. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Hyaluronic acid (HA), also known as hyaluronic acid, is ubiquitous in the interstitium of vertebrate tissues and some bacterial capsules, and its relative molecular mass (M r ) is usually 1×10 5 ~1×10 7 . HA has special physical and chemical properties and unique physiological functions, and has been widely used in many fields such as disease diagnosis and treatment, cosmetics, and health food. HA is degraded to generate different lower mo...

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Problems solved by technology

However, the inventors have found that while NIRS has the above-mentioned advantages, it also has some limitations, which limit its application.

For example: (1) NIRS analysis must use similar samples to establish a model first, and the establishment of the model requires a certain amount of energy and time; (2) Since NIRS analysis technology belongs to the second-level method, the accuracy of its measurement is limited to a certain extent Rely on the reference method; (3) The analysis of trace substances is not accurate enough, which is mainly caused by the detection of substances at 10000-4000cm -1 The absorption intensity in the spectral region is relatively weak

[0008] In addition, the inventors found that although NIRS technology can collect samples in the form of clear liquid, viscous liquid, solid, semi-solid, etc., without changing the shape of the sample to be tested, it often needs to overcome different difficulties in its practical application. Especially for the more complex viscous liquid environment, compared with the simple detection of solid form, more and more difficult problems need to be overcome

For example, when the NIRS technology is applied to the detection of the fermentation process, there are often large experimental errors. The main reason for the error is that the composition of the fermentation broth is complex, containing a large amount of bacteria, glucose, protein, etc. The complex background information will It has a great impact on the model; the viscous fermentation broth contains a large number of air bubbles, resulting in changes in the optical path

The inconsistency of the degree of alcohol precipitation in the treatment of fermentation broth leads to inaccurate primary data, thus introducing errors

Moreover, the model established by NIRS technology often depends on the consistency of the establishment conditions, so it is not widely applicable

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