Preparation method of rabies inactivation vaccine for livestock and stabilizing agent contained in vaccine

Abstract

The invention provides a preparation method of a rabies inactivation vaccine for livestock and a stabilizing agent contained in the vaccine. According to the preparation method, a virus concentrationprocess is that (1) a virus supernate continuously penetrates through a 300 KD membrane bag at the flow speed of 0.8-1 L / min, the virus is concentrated until the volume of the virus is 1 / 50-1 / 40 of the original volume, and coarse concentration liquid is obtained; (2) a PBS solution with the volume 10-15 times larger than that of the coarse concentration liquid is added into the coarse concentration liquid, the mixture penetrates through the 300 KD membrane bag at the flow speed of 1.8-2 L / min under the concentration pressure of 0.5-1.0 bar, under the condition that the mixture is concentrateduntil the volume of the mixture is 1 / 4-2 / 5 of the original volume of the mixture before penetration, the flow speed is adjusted to 0.8-1 L / min, and continuous concentration is conducted until the volume of the mixture is the same as the original volume of the coarse concentration liquid. According to the preparation method, by optimizing the virus concentration process, different membrane-penetration flow speeds are adopted for different antigen concentrations, in the concentration process, 30-50% of impure protein can be removed, the virus titer is kept at 90% or above, and the prepared vaccine is more stable and more efficient.

Description

Technical field [0001] The invention relates to the field of biological products, in particular to a preparation method of an inactivated rabies vaccine for animals and a stabilizer contained in the vaccine. Background technique [0002] Rabies (Rabies) is a zoonotic infection caused by the rabies virus (Rabiesvirus) of the Rhabdoviridae family and the genus Rabies virus. It is also known as waterphobia, rabies, etc. It is a kind of damage to the central nervous system. Acute viral infectious disease, all warm-blooded animals, including humans, can be infected. The disease is mainly infected by rabies virus liquid carried in animal saliva invading the body. Dogs are the main storage host and transmission vector of rabies virus in my country . The incubation period of rabies is generally from half a month to three months, most of which are concentrated in 30 to 90 days. Once rabies occurs, its development speed is very fast, mostly in 3 to 5 days, rarely more than 10 days, the case...

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a preparation method of veterinary rabies inactivated vaccine and the stabilizer contained in the vaccine, by optimizing the concentration and purification operations in the production process, and using The specific stabilizer overcomes the defects of low purity of rabies vaccine antigen in the prior art, and difficulty in balancing stability and quality

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