Tissue-engineered auricle-shape composite scaffold and preparation method thereof

Abstract

The invention relates to a tissue-engineered auricle-shape composite scaffold and a preparation method thereof. The method comprises the following steps of: preparing cartilage acellular matrix powderfrom cartilage tissue, preparing a gelatin or collagen suspension containing the cartilage acellular matrix, and constructing a three-dimensional digital model with a human ear shape through CT, MRIor laser scanning and computer-aided design; preparing a three-dimensional auricle grid frame with a human ear shape by 3D printing of a PCL base material, and preparing a solid male die and a solid female die corresponding to the auricle shape; fixing the three-dimensional auricle grid frame with a human ear shape at a middle position between the solid male die and the solid female die, perfusingthe prepared gelatin or collagen suspension containing the cartilage acellular matrix, performing freezing treatment after perfusion, stripping the solid male die and the solid female die after freezing to obtain a three-dimensional frozen scaffold, freezing and drying the three-dimensional frozen scaffold, soaking the three-dimensional frozen scaffold into a chemical cross-linking agent for cross-linking, repeatedly washing and soaking the scaffold with deionized water after the cross-linking to remove residual chemical cross-linking agent, and performing vacuum freeze drying to obtain the final three-dimensional auricle-shape scaffold.

Description

technical field [0001] The invention relates to biomedical tissue engineering, in particular to a tissue engineering auricle shape support with a three-dimensional structure and a preparation method thereof. Background technique [0002] Auricle defects or loss due to various reasons such as congenital microtia, trauma and tumors are common clinically. The auricle is one of the main features of the human face, and its defect or loss will seriously affect the patient's aesthetics and mental health. The auricle of the human body is mainly composed of skin and cartilage. Since the skin tissue is easily obtained by local skin expansion and / or skin grafting, the key difficulty in ear reconstruction for auricle defects lies in how to obtain an ear with good shape and function. Rebuild the bracket. The current clinical ear reconstruction methods are mainly based on autologous costal cartilage carving or high-density polyethylene (HDPE) artificial ear brackets. The disadvantages a...

AI Technical Summary

Problems solved by technology

At present, polyglycolic acid / polylactic acid (polyglycolic acid / polylactic acid, PGA / PLA) scaffolds combined with large animal chondrocytes have been used to successfully construct cartilage with accurate human ear shape in vitro, but long-term observation in vivo has found that due to This scaffold is a polymer synthetic material, and the small amount of undegraded scaffold remaining in the cartilage can easily cause aseptic inflammatory reactions, resulting in unstable cartilage regeneration in vivo

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