Temperature-tolerant yeast and separation method and application thereof

A technology of separation culture and temperature resistance, which is applied in the field of wine making, can solve the problems of lack of temperature resistance, achieve good temperature resistance, improve the rate of high-quality products and wine yield, and highlight the effects of ethanol production and esterification

Inactive Publication Date: 2019-09-06
JIANGSU KINGS LUCK BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the various types of yeast disclosed in the prior art do not have good temperature resistance. Therefore, yeasts with good temperature resistance and excellent fermentation have been selected and bred, that is, as the fermenta

Method used

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  • Temperature-tolerant yeast and separation method and application thereof
  • Temperature-tolerant yeast and separation method and application thereof
  • Temperature-tolerant yeast and separation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0048] This embodiment provides a kind of temperature-resistant yeast, and the isolation and cultivation method comprises the following steps:

[0049] (1) Put 10 g of Luzhou-flavor medium-high-temperature Daqu on the day of entering the room into a triangular flask filled with 90 mL of sterile water, and shake it for 30 minutes to obtain a bacterial suspension;

[0050] (2) Dilute the bacterial suspension obtained in step (1) by the 10-fold method, apply the rolling ball method on the sterile modified solid medium, and cultivate at 28° C. for 3 days to obtain colonies;

[0051] (3) Pick the colony with regular shape in step (2) and carry out 10 streak cultures on the sterile modified solid medium to separate and purify, and obtain a single pure colony, which is confirmed as yeast by microscopic examination, and 26S rDNA sequence Saccharomyces cerevisiae, that is, the temperature-resistant yeast of the present invention, is screened out by homology analysis, and stored on the ...

Embodiment 2

[0056] This embodiment provides a kind of temperature-resistant yeast, and the isolation and cultivation method comprises the following steps:

[0057] (1) Put 10 g of Luzhou-flavor medium-high-temperature Daqu fermented for 1 day in a triangular flask filled with 90 mL of sterile water, and shake for 30 minutes to obtain a bacterial suspension;

[0058] (2) Dilute the bacterial suspension obtained in step (1) by the 10-fold method, apply the rolling ball method on a sterile modified solid medium, and cultivate at 26° C. for 4 days to obtain colonies;

[0059] (3) Pick the colony with regular shape in step (2) and carry out 5 times of streak culture on sterile modified solid medium to separate and purify, and obtain a single pure colony, which is confirmed as yeast by microscopic examination, and 26S rDNA sequence is used Saccharomyces cerevisiae, that is, the temperature-resistant yeast of the present invention, is screened out by homology analysis, and stored on the improved...

Embodiment 3

[0061] This embodiment provides a kind of temperature-resistant yeast, and the isolation and cultivation method comprises the following steps:

[0062] (1) Put 10 g of Luzhou-flavor medium-high-temperature Daqu fermented for 5 days in a triangular flask filled with 90 mL of sterile water, and shake it for 30 minutes to obtain a bacterial suspension;

[0063] (2) Dilute the bacterial suspension obtained in step (1) by the 10-fold method, apply the rolling ball method on the sterile modified solid medium, and cultivate at 30° C. for 2 days to obtain colonies;

[0064] (3) Pick the colony with regular shape in step (2) and perform 8 streak cultures on the sterile modified solid medium to separate and purify, and obtain a single pure colony, which is confirmed as yeast by microscopic examination, and 26S rDNA sequence Saccharomyces cerevisiae, that is, the temperature-resistant yeast of the present invention, is screened out by homology analysis, and stored on the improved solid s...

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Abstract

The invention relates to a temperature-tolerant yeast and a separation method and application thereof. The temperature-tolerant yeast is named as a saccharomyces cerevisiae YP005A strain, the preservation unit is General Microbial Center of the Commission for the Protection and Management of Microbial Species in China, the preservation time is Feb. 25th , 2019, the preservation number is CGMCC No.17256, and the address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The temperature-tolerant yeast is good in temperature tolerance, good fermentation performance at lower temperature and higher temperature can be maintained, the weight loss of CO2 is kept at a high level in the fermentation process at lower temperature and higher temperature, and meanwhile, the saccharomyces cerevisiae has outstanding ethanol production and esterification capacity at lower temperature and higher temperature; moreover, the strain can effectively improve the quality of highly flavored type medium and high temperature yeast for making hard liquor, so that the production of the highly flavored liquor is of great significance for improving the quality rate of a wine base and wine productionrate.

Description

technical field [0001] The invention belongs to the technical field of brewing, and in particular relates to a yeast and its separation and cultivation method and application, in particular to a temperature-resistant yeast and its separation and cultivation method and application. Background technique [0002] Baijiu is a type of alcohol that has been passed down by the Chinese for thousands of years, and it is also the type of alcohol that Chinese people drink most often. There are also many studies on its brewing process. Yeast is the main flora in the fermentation process of baijiu. [0003] CN108148829A discloses a method for preparing novel Saccharomyces cerevisiae by genome recombination technology and the obtained novel Saccharomyces cerevisiae, which can prepare yeast strains with excellent ethanol tolerance. The program includes Saccharomyces cerevisiae and alcohol-resistant and low-acetaldehyde-producing yeast strains as starting strains, using optimized sporulatio...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N1/02C12G3/02C12R1/865
CPCC12G3/02C12N1/02C12N1/18C12N1/185C12R2001/865
Inventor 吴建峰季方袁小转刘梦夏胡佳佳
Owner JIANGSU KINGS LUCK BREWERY
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