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Bacillus mucilaginosus and application thereof for preparing saline alkali soil improvement agent

A technology for Bacillus colloids and saline-alkali soil, which is applied in the field of agricultural microorganisms, can solve the problems of repairing speed and scope of application that cannot meet actual needs, limited bacterial strains such as salt-alkaline resistance and high-temperature resistance, and achieves repairing and improving salt. Alkaline soil, enhance self-healing ability, promote dissolution effect

Active Publication Date: 2019-08-09
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the above-mentioned technical solutions have a certain repair function on the soil after acting on saline-alkali land, they are limited by the salt-alkaline and high-temperature resistance of the strain itself, resulting in a restoration speed and scope of application that cannot meet actual needs.

Method used

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  • Bacillus mucilaginosus and application thereof for preparing saline alkali soil improvement agent
  • Bacillus mucilaginosus and application thereof for preparing saline alkali soil improvement agent
  • Bacillus mucilaginosus and application thereof for preparing saline alkali soil improvement agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The screening process of Bacillus mucilaginosus JDzhs-bh is as follows:

[0056] (1) Retrieve soil samples from Binzhou Zhanhua saline-alkali land test base, screen Bacillus colioids according to the screening medium and conditions of Bacillus colioids, separate and purify, and finally obtain pure strains;

[0057] The screening medium is a silicate medium with the following components:

[0058] Sucrose 5g, Na 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, FeCl 3 0.005g, CaCO 3 0.1g, bauxite 0.5g, agar 15g, water 1000ml; pH 7.5;

[0059] Screening conditions: cultured at 25°C for 4 days;

[0060] Whether it is a pure strain is judged by microscope observation, and the pure strain is obtained by the method of streaking on a plate;

[0061] (2) prepare the bacterial suspension of bacillus colloidus bacterial strain

[0062] Pick a single colony of the strain and insert it into a simple medium, the components are as follows:

[0063] Yeast extract powder 5g / L, peptone 10g / L...

Embodiment 2

[0115] The culture method of colloid bacillus (Bacillus mucilaginosus) JDzhs-bh, comprises the steps:

[0116] (1) Inoculate Bacillus colloidus into the slant medium, activate and cultivate at a temperature of 28° C. for 48 hours to obtain an activated strain;

[0117] The components of the slant medium are as follows:

[0118] Sucrose 15g / L, K 2 HPO 3 3.2g / L, NaCl 2.6g / L, SiO 2 7g / L, MgSO 4 3.0g / L, CaCO 3 5.5g / L, yeast extract 2.7g / L, agar 15g / L, pH 7.4;

[0119] (2) Inoculate the activated bacterial strain prepared in step (1) in the seed medium, and cultivate it for 20 hours at 30°C and 180r / min to obtain the seed solution;

[0120] Described seed culture medium component is as follows:

[0121] Starch 13g / L, K 2 HPO 3 8g / L, ammonium sulfate 3.8g / L, yeast extract 2.7g / L, sucrose 7g / L, MgSO 4 3.5g / L, CaCO 3 3.5g / L, SiO 2 6g / L, FeCl 3 0.5g / L, pH 7.4;

[0122] (3) inoculate the seed solution prepared in step (2) in the fermentation medium, the inoculatio...

Embodiment 3

[0127] A saline-alkali soil improver, prepared by the following components:

[0128] 5-12 parts of plant ash, 24-32 parts of zeolite, 10-18 parts of humic acid, 2-6 parts of Paecilomyces lilacinus bacteria liquid, 12-42 parts of attapulgite, 12-20 parts of xanthan gum, gum 4-8 parts of Bacillus JDzhs-bh bacteria liquid;

[0129] After the above components are mixed, a soil conditioner is made.

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Abstract

The invention relates to bacillus mucilaginosus and application thereof for preparing a saline alkali soil improvement agent. The bacillus mucilaginosus JDzhs-bh is preserved at the China Center for Type Culture Collection on March 20th, 2019, the address is No.3, courtyard 1, the western Beichen road, Chaoyang district of Beijing, and the strain preservation number is CGMCC No.17376. The invention discloses bacillus mucilaginosus capable of resisting high temperature and saline alkali and being cultivated through ultraviolet mutation screening for the first time. The bacillus mucilaginosus has the stress resistance for high temperature and saline alkali, and can effectively adapt to saline alkali hardening soil. Meanwhile, the effect of degrading silicon and phosphorus is achieved, release of elements such as soluble silicon and phosphorus in soil is promoted, crop stress resistance is enhanced, the utilization rate of the soil repairing agent is achieved, saline alkali soil is repaired and improved, and salinization is lowered.

Description

technical field [0001] The invention relates to a colloidal bacillus strain and its application in preparing a saline-alkali soil improver, belonging to the technical field of agricultural microorganisms. Background technique [0002] Soil salinization and secondary salinization have become resource constraints for the sustainable development of irrigated agriculture in the world. The problem of soil salinization exists widely in the world, especially in arid and semi-arid areas. Soil salinization is the result of the combination of natural factors and human activities. Natural factors mainly refer to abnormal climate conditions, especially severe drought conditions, resulting in vegetation degradation, accelerated wind erosion, and saline-alkali desertification. Human factors mainly refer to overgrazing, deforestation, grassland reclamation and continuous farming, etc. In the past ten years, irrational use of chemical fertilizers and abuse of pesticides are also an import...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/40C12R1/07C09K101/00
CPCC09K17/40C09K2101/00C12N1/20C12N1/205C12R2001/07
Inventor 姚强董晓霞宫志远辛寒晓孙中涛韩建东黄春燕张元祺刘盛林刘青安森魏秀萍李慧琳亓超凡
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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