Detection kit and detection method for aspartate aminotransferase mitochondrial isozyme

Abstract

The invention provides a detection kit and a detection method for an aspartate aminotransferase mitochondrial isozyme. The kit consists of a reagent R1 and a reagent R2 which are mutually independent;the reagent R1 is prepared from the following components: 12-13g / L of TRIS buffer, 40-60g / L of L-potassium aspartate, 2.5-4KU / L of malate dehydrogenase, 15mg / L-25mg / L of anti-cell type AST monoclonalantibody, and 6-8g / L of NADH; a solvent for the reagent R1 is purified water; the reagent R2 prepared from the following components: 12-13g / L of TRIS buffer and 10-12g / L of alpha-ketoglutaric acid; asolvent for the reagent R2 is purified water. The detection kit and the detection method provided by the invention have the advantages of good quantitative ability, short time consumption, rapid batch detection, good repeatability, good stability, simple operation and low price, and can be applied to multi-brand clinical biochemical analyzers with open channels.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit and a detection method for mitochondrial isozymes of aspartate aminotransferase. Background technique [0002] Aspartate aminotransferase (AST) is widely distributed in the heart, liver, lung, skeletal muscle, kidney, pancreas and other tissue cells. The highest content in cardiomyocytes, followed by liver cells. AST has two isozymes, one is the cytoplasmic isozyme of aspartate aminotransferase (s-AST) located in the cytoplasm; the other is the mitochondrial isozyme of aspartate aminotransferase located in the mitochondria of cells (m-AST). The changes in the concentrations of the two isoenzymes in serum have different clinical significance, among which the m-AST isoenzyme can objectively reflect the severity of some diseases and serve as an indicator for guiding treatment and prognosis of some diseases. In recent years, there have been more and more studies on m-A...

AI Technical Summary

Problems solved by technology

In recent years, there have been more and more studies on m-AST. The existing m-AST detection methods are mainly as follows: due to the low content of m-AST, the electrophoresis method requires a complicated dyeing process and a high-precision densitometer. Not suitable for clinical large-scale sample analysis; ion exchange column chromatography, this method is not suitable for large-scale operations; enzyme immunoassay has a low degree of automation; radioimmunoassay has radioactive contamination; Problems with routine project development

The above detection methods are cumbersome to operate, have low precision, poor quantitative ability, and take a long time, so they cannot be quickly detected.

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