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Method for improving enzymatic hydrolysis efficiency by adopting hydrothermal-microwave approach combined with ionic liquid for two-step pretreatment of lignocellulose raw material

A lignocellulose and ionic liquid technology, applied in the field of biomass energy utilization, can solve the problems of reduced concentration of tetrabutylammonium hydroxide, difficult temperature control, unfavorable sustainable mass production and the like

Inactive Publication Date: 2019-07-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This also indicates that if you want to dissolve more cellulose, you need to provide a high concentration of tetrabutylammonium hydroxide [TBA][OH], which consumes more solvent, and high concentration means that the dissolution process of cellulose will be accompanied by high energy consumption Concentration process, which in turn leads to the reduction of tetrabutylammonium hydroxide [TBA][OH] due to concentration, which is not conducive to sustainable mass production
In this regard, as in patent 201510093034, by stirring and dissolving at different temperatures, tetrabutylammonium hydroxide [TBA][OH] can effectively dissolve cellulose under the condition of a mass concentration of 35-50%; however, continuous heating, cooling, Heating up again, although the mass concentration of tetrabutylammonium hydroxide [TBA] [OH] has been reduced, the steps are loaded down with trivial details, and it is difficult to control the temperature in the reaction process

Method used

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  • Method for improving enzymatic hydrolysis efficiency by adopting hydrothermal-microwave approach combined with ionic liquid for two-step pretreatment of lignocellulose raw material
  • Method for improving enzymatic hydrolysis efficiency by adopting hydrothermal-microwave approach combined with ionic liquid for two-step pretreatment of lignocellulose raw material

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Using hydrothermal pretreatment of lignocellulosic raw materials to improve enzymatic hydrolysis efficiency

[0031] 1) pulverizing the lignocellulose raw material into 100 mesh lignocellulose powder;

[0032]2) Accurately weigh 5 g of the lignocellulose powder obtained in step 1 and mix it with 50 mL of distilled water and heat it in a water bath for 2 hours at a temperature of 90°C; after the heating is completed, filter and dry to obtain a sample after hydrothermal pretreatment;

[0033] 3) Accurately weigh 4 g of sample I obtained in step 2, place it in a 50 mL Erlenmeyer flask, add 20 mL of sodium citrate buffer solution (pH=4.8), and simultaneously add cellulase 55 FPU / g substrate, and the amount of β-glucosidase is 75FPU / g substrate, sealed with plastic wrap and placed in a constant temperature oscillator at 180rpm / min, 55°C for 72h; then cooled to room temperature, 1mL of supernatant was taken, and the content of reducing sugar in the enzymatic soluti...

Embodiment 2

[0035] Example 2: Using [TBA][OH] in combination with distilled water to pretreat lignocellulosic raw materials to improve enzymatic hydrolysis efficiency

[0036] 1) pulverizing the lignocellulose raw material into 100 mesh lignocellulose powder;

[0037] 2) Accurately weigh 5g of the lignocellulose powder obtained in step 1, place it in a 75mL Erlenmeyer flask, add 40mL of ionic liquid and 10mL of distilled water at the same time, place it in a water bath and heat for 40min at a temperature of 60°C;

[0038] 3) After heating, centrifuge the solid-liquid mixture obtained in step 2 at a speed of 15000r / min for 10min, separate the solid from the liquid, and take the supernatant;

[0039] 4) Add absolute ethanol to the supernatant obtained in step 3 at a volume ratio of 1:3. Obtain the mixed solution containing the precipitate, dry and store the obtained precipitate after suction filtration, that is, obtain the sample after the hydrothermal pretreatment of the ionic liquid tetr...

Embodiment 3

[0043] Example 3: Using microwaves combined with distilled water to pretreat lignocellulosic raw materials to improve enzymatic hydrolysis efficiency

[0044] 1) pulverizing the lignocellulose raw material into 100 mesh lignocellulose powder;

[0045] 2) Accurately weigh 5g of the sample I obtained in step 1, put it in a 75mL conical flask, add 50mL distilled water at the same time, put it in a microwave reactor, set the microwave power to 700w, the microwave frequency to 2450HHz, and the time to 20min. dry to obtain a sample pretreated by microwave combined with distilled water;

[0046] 3) Accurately weigh 4 g of the sample obtained in step 2, place it in a 50 mL Erlenmeyer flask, add 20 mL of sodium citrate buffer solution (pH=4.8), and simultaneously add cellulase 55 FPU / g substrate, and the amount of β-glucosidase is 75FPU / g substrate, sealed with plastic wrap and placed in a constant temperature oscillator at 180rpm / min, 55°C for 72h; then cooled to room temperature, 1m...

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Abstract

The invention relates to the field of biomass pretreatment and provides a method for improving the enzymatic hydrolysis efficiency by adopting a hydrothermal-microwave approach combined with an ionicliquid for two-step pretreatment of a lignocellulose raw material. The method can effectively improve the enzymatic hydrolysis efficiency of the lignocellulose raw material. The pretreatment method aims to achieve the effects that the method is environmentally friendly, the material is recyclable and the energy consumption and the cost are low, and the use rate of the ionic liquid is increased. Byadopting the hydrothermal-microwave approach combined with the ionic liquid tetrabutylammonium hydroxide [TBA][OH] for carrying out pretreatment sequentially on the lignocellulose raw material, the lignocellulose raw material is effectively destructed, the wetting effect of tetrabutylammonium hydroxide [TBA][OH] on lignocellulose is enhanced, more tetrabutylammonium hydroxide [TBA][OH] enters thelignocellulose through destroyed gaps in the surface of the lignocellulose, structures of hemicellulose and lignin are broken, depolymerization among the hemicellulose, the lignin and cellulose occurs, and correspondingly the solubility degree of tetrabutylammonium hydroxide [TBA][OH] on the cellulose is increased.

Description

technical field [0001] The invention belongs to the field of biomass energy utilization, and relates to a method for improving enzymolysis efficiency by hydrothermal-microwave combined with ionic liquid two-step pretreatment of lignocellulosic raw materials Background technique [0002] With the progress of human society and the rapid development of economy, the demand for various resources such as fuels and chemicals is increasing day by day. The existing fossil resources on the earth, such as coal, oil, natural gas, etc., can no longer meet the needs of human beings for sustainable development. Demand, coupled with the long-term use of various harmful gases released by the burning of fossil fuels, has become increasingly prominent in the impact of global environmental pollution and climate warming. Therefore, it is imminent to find renewable and clean energy to replace traditional fossil fuels. Biomass is the most abundant renewable energy source on Earth and is used to pr...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14C12P2201/00
Inventor 侯贤锋高振忠王晓波李锰王淑捷百明阳
Owner SOUTH CHINA AGRI UNIV
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