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A kind of preparation method of composite bone repair material

A bone repair and freeze-dried bone technology, applied in the field of biomedical engineering, can solve problems such as deep wound infection, affecting surgical efficacy, and graft settlement

Active Publication Date: 2021-09-14
FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is undeniable that there are some obvious problems in the application of BMP-2, such as fatal anterior neck edema, deep wound infection, heterotopic ossification formation, early postoperative vertebral body osteolysis, graft subsidence, transplantation, etc. object displacement, postoperative neuritis, etc.
Among them, in the application of spinal intervertebral fusion, due to the high concentration of local BMP-2, the phenomenon of bone resorption of the implanted early endplate is common. This kind of bone resorption can easily lead to the subsidence or displacement of the graft, and then affect the curative effect of the operation.

Method used

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  • A kind of preparation method of composite bone repair material
  • A kind of preparation method of composite bone repair material
  • A kind of preparation method of composite bone repair material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of the composite bone repair material of this embodiment comprises the following steps:

[0034]Step 1. Preparation of allogeneic freeze-dried bone (FDB): remove the soft tissue of the donor, cut the bone into 3-8mm×3-8mm×3-8mm, freeze at -80°C for 5w, initially remove the antigenicity, and further remove Clean soft tissue, degrease with supercritical carbon dioxide according to the content recorded in the patent number ZL201510134565.3, vacuum freeze-dry at -40°C for 72 hours, and sterilize by irradiation;

[0035] Step 2, prepare hot dog ridge polar extract (PRCHP) solution:

[0036] 1) Prepare scalded dog ridge: take the cleaned dog ridge slices of uniform size, put 8 times the amount of river sand in a pot, heat to control the temperature of the river sand at 180°C, put in the dog ridge slices, fry and bury them , fry for 6.5 minutes, take out and sieve to remove sand, let cool, set aside;

[0037] 2) Polysaccharide extract of dog ridge: tak...

Embodiment 2

[0043] The preparation method of the composite bone repair material of this embodiment comprises the following steps:

[0044] Step 1. Preparation of allogeneic freeze-dried bone (FDB): same as in Example 1;

[0045] Step 2, prepare hot dog ridge polar extract (PRCHP) solution:

[0046] 1) Prepare scalded dog ridge: take the cleaned dog ridge slices of uniform size, put 8 times the amount of river sand in a pot, heat to control the temperature of the river sand at 190°C, put in the dog ridge slices, fry and bury them , fry for 6 minutes, take out the sieve to remove the sand, let cool, and set aside;

[0047] 2) Polysaccharide extract of dog ridge: take scalded dog ridge of uniform size, crush it through a 100-mesh sieve to obtain hot dog ridge powder, decoct it with 8 times the amount of water for 40 minutes each time, and combine the decoction liquid , concentrated under reduced pressure, added ethanol to make the final concentration 80%, kept overnight, and obtained crude...

Embodiment 3

[0053] The preparation method of the composite bone repair material of this embodiment comprises the following steps:

[0054] Step 1. Preparation of allogeneic freeze-dried bone (FDB): same as in Example 1;

[0055] Step 2, prepare hot dog ridge polar extract (PRCHP) solution:

[0056] 1) Prepare scalded dog ridge: take the cleaned dog ridge slices of uniform size, put 8 times the amount of river sand in a pot, heat to control the temperature of the river sand at 180°C, put in the dog ridge slices, fry and bury them , fry for 6 minutes, take out the sieve to remove the sand, let cool, and set aside;

[0057] 2) Polysaccharide extract of dog ridge: take hot dog ridge of uniform size, crush it through a 60-mesh sieve to obtain hot dog ridge powder, decoct it twice with 6 times the amount of water, 40 minutes each time, and combine the decoction liquid , concentrated under reduced pressure, added ethanol to make the final concentration 80%, kept overnight, and obtained crude p...

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Abstract

The embodiment of the invention discloses a preparation method of a composite bone repair material, which belongs to the technical field of biomedical engineering. The composite bone repair material is made of allogeneic freeze-dried bone (FDB), hot dog ridge polar extract (PRCHP) solution, and bone morphogenetic protein 2 (BMP‑2). The composite bone repair material of the embodiment of the present invention uses FDB as a scaffold material, and is compounded with RHCHP and BMP-2. RHCHP cooperates with BMP-2 to promote osteogenesis and inhibit osteoclastosis. On the basis of ensuring that the osteogenic activity of BMP-2 is not reduced On the one hand, reduce its local application concentration as much as possible, reduce cytotoxicity, so as to achieve the effect of promoting bone formation and reducing complications. The present invention adopts soaking and freeze-drying self-assembly technology to complete the composite steps of PRCHP-FDM-BMP-2, and has the advantages of simple operation, easy control and low production cost.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of biomedical engineering, and in particular to a preparation method of a composite bone repair material. Background technique [0002] Spinal fusion plays an important role in restoring spinal stability, restoring normal alignment of the spine, and relieving pain. In recent years, with the continuous improvement and development of spinal internal fixation devices and bone graft materials, the fusion rate of bone grafts has increased significantly, but the stability of the spine can only be achieved through bony fusion. Bone graft materials provide a suitable environment for the growth of cells and tissues during bone graft fusion, and are continuously degraded during the growth of new tissues and cells, and are finally replaced by new bone tissues. [0003] The fusion rate of autologous bone grafting in spinal fusion can reach more than 90%, but this method has the disadvantages o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/22A61L27/50
CPCA61L27/227A61L27/3608A61L27/3637A61L27/365A61L27/3691A61L27/50A61L2300/30A61L2300/412A61L2430/02A61L2430/40C08L89/00
Inventor 李忠海徐钢赵彦涛王博张瑜常煜昂
Owner FIRST AFFILIATED HOSPITAL OF DALIAN MEDICAL UNIV
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