Composition and use thereof in protein extraction and/or dissolution

A protein extraction and composition technology, applied in the field of extraction and/or dissolution, can solve the problems of accurate understanding and in-depth understanding of the outer membrane of Geobacter sulfur-reducing, inactivation of catalytic subunits, etc., to avoid protein aggregation , easy to operate, avoid the effect of deactivation

Active Publication Date: 2019-06-14
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the particularity of outer membrane cytochrome c, sodium dodecyl sulfate will lead to irreversible aggregation of lipophilic protein at high temperature, and the use of reducing agent will also completely inactivate the catalytic subunit of cytochrome c
These issues limit researchers' accurate knowledge and in-depth understanding of the outer membrane of Geobacter sulfurreducing

Method used

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  • Composition and use thereof in protein extraction and/or dissolution
  • Composition and use thereof in protein extraction and/or dissolution
  • Composition and use thereof in protein extraction and/or dissolution

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1, Extraction and Characterization of Cytochrome c Components in the Outer Membrane of Geobacter Sulfurreducens Under Anaerobic Conditions

[0034] The mineral salt medium formula used in this example is as follows: 20mM sodium acetate, 40mM fumaric acid, 1.50g / L ammonium chloride, 0.60g / L disodium hydrogen phosphate, 0.10g / L potassium chloride, 2.50g / L carbonic acid Sodium hydrogen and 1% trace element solution. Trace element solution includes 1.50g / L nitrilotriacetic acid, 3.00g / L magnesium sulfate heptahydrate, 0.50g / L manganese sulfate hydrate, 1.00g / L sodium chloride, 0.10g / L ferrous sulfate heptahydrate, 0.18g / L cobalt sulfate heptahydrate, 0.10g / L calcium chloride dihydrate, 0.18g / L zinc sulfate heptahydrate, 0.01g / L copper sulfate pentahydrate, 0.02g / L potassium aluminum sulfate dodecahydrate, 0.01g / L boric acid , 0.01g / L sodium molybdate dihydrate, 0.03g / L nickel chloride hexahydrate, 0.30mg / L sodium selenite pentahydrate, 0.40mg / L sodium tungstate di...

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Abstract

The invention relates to the technical field of protein extraction, and in particular to a composition and use thereof in protein extraction and / or dissolution. The composition is composed of urea thiourea Triton X-100 and CHAPS. While the composition contains a plurality of denaturants which can perform affinity with a surface hydrophobic subunit of a strongly hydrophobic protein in an ampholyte,a tertiary structure of a protein peptide chain is opened, the deactivation of catalytic subunits is avoided, and the protein agglomeration is also avoided. Therefore, the separation treatment of thestrongly hydrophobic protein is achieved, and the solubility of the protein is improved. The composition has simple composition, is convenient to operate and has a remarkable effect.

Description

technical field [0001] The invention relates to the technical field of protein extraction, in particular to a composition and its application in protein extraction and / or dissolution. Background technique [0002] Protein is an important class of organic macromolecules in organisms and a direct participant in the execution of functions in organisms. Proteins can play a variety of functions in the body, such as structural support, reaction catalysis, growth regulation, material transport and storage. Membrane proteins are a special class of proteins, which are usually distributed on the surface of the outer membrane of cells or embedded in the outer membrane of cells. Since membrane proteins are usually embedded in the phospholipid bilayer of the cell membrane, their lipid solubility is better than that of intracellular proteins. Membrane proteins usually have more hydrophobic residues on the surface, while hydrophilic residues are usually buried inside the protein. Such st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/34
Inventor 俞汉青马昕李道波
Owner UNIV OF SCI & TECH OF CHINA
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