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Fullerene-based fluorescent probe with rapid and highly-efficient response to glutathione, and preparation method and application thereof

A fluorescent probe and glutathione technology, applied in the field of protein detection in analytical chemistry, can solve problems such as limited sensitivity and detection range, long reaction time under reaction conditions, expensive chromatography instruments, etc., to increase hydrophilicity, The effect of improving sensitivity and improving responsiveness

Active Publication Date: 2019-06-07
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional glutathione concentration detection such as enzymatic method and chromatography method can detect glutathione concentration, but there are many shortcomings, such as enzymatic method requires specific reaction conditions and long reaction time; chromatography requires expensive instruments, High requirements on the operator, and limited sensitivity and detection range

Method used

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  • Fullerene-based fluorescent probe with rapid and highly-efficient response to glutathione, and preparation method and application thereof
  • Fullerene-based fluorescent probe with rapid and highly-efficient response to glutathione, and preparation method and application thereof
  • Fullerene-based fluorescent probe with rapid and highly-efficient response to glutathione, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A fast and efficient glutathione-responsive fluorescent probe based on fullerene expressed as FQD-MnO 2 ; Wherein, FQD is fullerene quantum dot, and structural formula is C 60 (OH) x (NH 2 ) y o z , x=13, y=7, z=15.

[0044] The excitation wavelength range of fullerene quantum dots described in this embodiment is 350-500nm, and the fluorescence emission spectrum of fullerene quantum dots is an excitation wavelength-dependent emission spectrum, and the scope of its maximum emission peak is 450-550nm. The point particle size is less than 10nm.

[0045] The fluorescent probe described in this embodiment is composed of fullerene quantum dots and manganese dioxide nanosheets through electrostatic adsorption and coordination bonds. The preparation method specifically comprises the following steps:

[0046] a. Synthesis of fullerene quantum dots: in C 60 Adding mass concentration to the powder is 30% hydrogen peroxide and 28% ammonia, every 10mg of C 60 Add 10ml of a ...

Embodiment 2

[0050] A fast and efficient glutathione-responsive fluorescent probe based on fullerene expressed as FQD-MnO 2 ; Wherein, FQD is fullerene quantum dot, and structural formula is C 60 (OH) x (NH 2 ) y o z , x=13, y=7, z=15.

[0051] The excitation wavelength range of fullerene quantum dots described in this embodiment is 350-500nm, and the fluorescence emission spectrum of fullerene quantum dots is an excitation wavelength-dependent emission spectrum, and the scope of its maximum emission peak is 450-550nm. The point particle size is less than 10nm.

[0052] The preparation method specifically comprises the following steps:

[0053] a. Synthesis of fullerene quantum dots: in C 60 Adding mass concentration to the powder is 30% hydrogen peroxide and 28% ammonia, every 10mg of C 60 Add 1ml of a mixture of hydrogen peroxide and ammonia water, wherein the volume ratio of hydrogen peroxide and ammonia water is 5:2. Stir in the open at 50°C for 12 hours, the black solid dissol...

Embodiment 3

[0064] In this example, the method for measuring the concentration of GSH with the fluorescent probe of the present invention is studied.

[0065] Figure 7 Fluorescence spectrum graph and linear correlation regression line for the GSH response of the sample. The probe (180 μg / mL) was quickly mixed with different concentrations of GSH solutions at room temperature to observe the changes in fluorescence. Under the condition of 0 GSH concentration, FQD-MnO 2 Little to no fluorescence. With the gradual increase of GSH concentration up to 1 mM, the fluorescence of quantum dots gradually recovered. Both the linear regression line and the equation prove that within the GSH concentration range of 0-1mM, the fluorescence intensity of the quantum dots is linearly related to the GSH concentration, and the calculation equation is FL=0.06285C GSH +13.815(r 2 = 0.992). Main mechanism: In this GSH-responsive fluorescent probe, manganese dioxide nanosheets can effectively quench the fl...

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Abstract

The invention discloses a fullerene-based fluorescent probe with rapid and highly-efficient response to glutathione, and a preparation method and an application thereof. The fluorescent probe is a complex based on a fullerene quantum dot and a manganese dioxide nanosheet; the fluorescent probe with response to the glutathione is formed through the actions of electrostatic adsorption and coordination, and is expressed as FQD-MnO2; and the fullerene quantum dot has a structural formula of C60(OH)x(NH2)yOz. The fluorescent probe provided by the invention is simple in synthesis, has stable fluorescence, is insusceptible to quenching, can rapidly respond to the glutathione (GSH), and can be used for quantitative detection of the content of GSH in a solution and a cell. Under the condition of the presence of the GSH, the manganese dioxide nanosheet is rapidly reduced to manganese ions, and fluorescence of FQD is recovered at the same time. Through construction of the linear relationship between the concentration of the GSH and the fluorescence recovery intensity of the FQD, the concentration of the glutathione in the solution and the cell can be quantitatively detected. The fullerene-based fluorescent probe provided by the invention can efficiently and rapidly detect the content of the GSH and has high sensitivity and wide measurement concentration range.

Description

technical field [0001] The invention belongs to the technical field of protein detection in analytical chemistry, in particular to a fullerene-based fast and efficient glutathione-responsive fluorescent probe and a preparation method and application thereof. Background technique [0002] C 60 Molecules are closed spheres composed of five-membered rings and six-membered rings, which have a highly symmetrical structure and therefore have unique physical and chemical properties, which have become the focus of research. Because of its safety and efficiency, C 60 As a carrier and photosensitizer, it is gradually applied in the field of biomedicine. Although C 60 Molecules are insoluble in water, and many researchers can solve the problem of water solubility by modifying the surface of fullerene with groups. C 60 The molecule has almost no fluorescence due to its high symmetry, and recent studies have shown that the highly symmetric structure of fullerene can be broken by oxi...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y40/00G01N21/64
Inventor 蔡挺彭亚运叶超
Owner CHINA PHARM UNIV
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