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Injectable silk fibroin porous hydrogel and preparation method thereof

A technology of porous hydrogel and silk fibroin, which is applied in the field of injectable silk fibroin porous hydrogel and its preparation, can solve problems such as mechanical properties and limitations of preparation methods, and achieves adjustable curing speed, good mechanical properties, Easy to inject effect

Active Publication Date: 2019-06-07
江西丝科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current silk fibroin hydrogels have many limitations in terms of mechanical properties and preparation methods. It is still a technology in this field to obtain injectable silk fibroin hydrogels without cross-linking agents, with excellent mechanical properties and porous structure. difficulty

Method used

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  • Injectable silk fibroin porous hydrogel and preparation method thereof
  • Injectable silk fibroin porous hydrogel and preparation method thereof
  • Injectable silk fibroin porous hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This example is the silk fibroin porous hydrogel prepared by the preparation method of the present invention.

[0052] The preparation method of the silk fibroin porous hydrogel described in this example is as follows:

[0053] (1) Preparation of silk fibroin aqueous solution, as shown in the schematic diagram figure 1 shown;

[0054] (2) The silk fibroin aqueous solution is perfused in the dialysis bag, and the water volatilization speed is controlled for concentration to obtain a silk fibroin concentrate, as shown in the schematic diagram. figure 2 The specification of the dialysis bag is 10Da, the ambient temperature during concentration is 0°C, the humidity is 10%, and the concentration of the silk fibroin concentrate is 20%;

[0055] (3) stirring and shearing the silk fibroin concentrate to make it pre-crosslinked to obtain a fluid foam of silk fibroin, the stirring speed is 1r / min, and the stirring time is 30s, as shown in the schematic diagram image 3 shown;...

Embodiment 2

[0065] This example is the silk fibroin porous hydrogel prepared by the preparation method of the present invention.

[0066] The preparation method of the silk fibroin porous hydrogel described in this example is as follows:

[0067] (1) prepare silk fibroin aqueous solution;

[0068] (2) The silk fibroin aqueous solution is poured into the dialysis bag, and the water volatilization speed is controlled for concentration to obtain the silk fibroin concentrate; The concentration of vegan protein concentrate is 30%;

[0069] (3) stirring and shearing the silk fibroin concentrate to make it pre-crosslinked to obtain a fluid foam of silk fibroin, the stirring speed is 200r / min, and the stirring time is 600s;

[0070] (4) The silk fibroin fluid foam is injected into the mold, and after standing, the injectable silk fibroin porous hydrogel is obtained, and the standing time is 5 hours.

[0071] Wherein, the specific preparation process of silk fibroin aqueous solution is as follo...

Embodiment 3

[0079] This example is the silk fibroin porous hydrogel prepared by the preparation method of the present invention.

[0080] The preparation method of the silk fibroin porous hydrogel described in this example is as follows:

[0081] (1) prepare silk fibroin aqueous solution;

[0082] (2) The silk fibroin aqueous solution is poured into the dialysis bag, and the water volatilization rate is controlled for concentration to obtain the silk fibroin concentrate; The concentration of vegan protein concentrate is 25%;

[0083] (3) stirring and shearing the silk fibroin concentrate to make it pre-crosslinked to obtain a fluid foam of silk fibroin, the stirring speed is 100r / min, and the stirring time is 300s;

[0084] (4) The silk fibroin fluid foam is injected into the mold, and after standing, the injectable silk fibroin porous hydrogel is obtained, and the standing time is 2 hours.

[0085] Wherein, the specific preparation process of silk fibroin aqueous solution is as follow...

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Abstract

The invention relates to an injectable silk fibroin porous hydrogel and a preparation method thereof, and belongs to the technical field of organic high-molecular polymer materials. The preparation method of the injectable silk fibroin porous hydrogel comprises the following steps: preparing a silk fibroin aqueous solution; performing concentration on the silk fibroin aqueous solution to obtain asilk fibroin concentrate; performing stirring shearing on the silk fibroin concentrate to make the concentrate pre-crosslinked to obtain silk fibroin fluid foam; and injecting the silk fibroin fluid foam into a mold, and allowing the mold to stand to obtain the injectable silk fibroin porous hydrogel. The preparation method provided by the invention simulates a molding process of natural silk without adding any cross-linking agents, and the operation is simple; the prepared hydrogel can fill the defect of any shapes, meet complex shapes of different wounds, and reduce invasiveness of an implant to body tissues; and before the hydrogel is injected, the hydrogel can be compounded with special-effects drugs and biologically-active substances through a mixing manner to construct a hydrogel drug sustained-release system.

Description

technical field [0001] The invention relates to an injectable silk fibroin porous hydrogel and a preparation method thereof, belonging to the technical field of organic polymer materials. Background technique [0002] Currently, the research of medical hydrogels has attracted widespread interest. Hydrogel materials have a variety of biological applications, including drug controlled release carriers and tissue engineering scaffolds, to repair defective tissues and organs. For tissue engineering scaffolds, they can often be divided into pre-formed porous scaffolds and injectable materials according to their molding characteristics. The former is to prefabricate biomaterials in vitro into a three-dimensional porous cell scaffold with a specific shape and a certain pore structure similar to the tissue or organ defect to be repaired, and then implant seed cells to construct the tissue, and then undergo surgery. The method of implanting this preformed system into the defect site...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08J9/00C08L89/00A61K9/06A61K47/42A61L27/22A61L27/52A61L27/54A61L27/56
Inventor 陶虎柳克银
Owner 江西丝科生物科技有限公司
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