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Biological material, preparation method thereof and application of biological material as bone defect repairing material

A technology of biomaterials and bioceramic materials, applied in the application of bone defect repair materials, biomaterials and their preparation, can solve the problems of limited printing height, yellowing of materials, and low printing accuracy of biomaterials

Active Publication Date: 2019-05-31
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention solves the problem of light-induced cross-linking molding of the opaque system of inorganic bioceramic particles in the prior art, as well as the technical problems of low printing accuracy of biological materials, limited printing height and yellowing of materials

Method used

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  • Biological material, preparation method thereof and application of biological material as bone defect repairing material
  • Biological material, preparation method thereof and application of biological material as bone defect repairing material
  • Biological material, preparation method thereof and application of biological material as bone defect repairing material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: C-CS 0.4g+Gel-MA 2.2g+HAP 5g+photocrosslinking+EDC / NHS crosslinking

[0033] The preferred embodiment provided by the present invention is an extruded 3D printing biomaterial for bone defect repair. The bio 3D printing paste formula includes an organic polymer part, an inorganic bioceramic particle part and a photoinitiating additive part.

[0034] The organic polymer part is composed of Gel-MA and C-CS. Take the C-CS 0.4g magnetic stirring dissolved in 11mLH 2 O, then add 2.2 g of the Gel-MA, and dissolve completely under stirring in a water bath at 50°C.

[0035] The photoinitiator additive part consists of photoinitiator 819 and photoinitiator 184 . Take the 0.15g of photoinitiator 819 and 0.25g of photoinitiator 184, and dissolve them in 0.4mL of acetone until they are completely dissolved, and the solution turns slightly yellow. The prepared photoinitiating additive is partially added to the organic polymer solution, and magnetically stirred until it ...

Embodiment 2

[0039] Example 2: C-CS 0.8g+Gel-MA 3.2g+photocrosslinking

[0040] In the biological 3D printing slurry component for bone repair of the present invention, the organic polymer part can be printed independently without relying on the inorganic particles.

[0041] Take 0.8g of the C-CS and dissolve it in 11mL of water with magnetic stirring, then add 3.2g of the Gel-MA, and dissolve completely in a 50°C water bath. The photoinitiator additive part consists of photoinitiator 819. Take 0.15 g of the photoinitiator 819 and dissolve it in 0.4 mL of acetone until completely dissolved.

[0042] The prepared photoinitiating additive is partially added to the organic polymer solution, and magnetically stirred until it dissolves completely to form a homogeneous solution.

[0043] Transfer the slurry to the barrel of the extrusion bioprinter, use a needle with an aperture of 0.16mm, set the air pressure to about 0.15MPa, the line speed to 6mm / s, set the line spacing to 0.3mm, and irradi...

Embodiment 3

[0044] Example 3: C-CS 0.4g+Gel-MA 2.2g+HAP 3g+glutaraldehyde crosslinking

[0045] Take the C-CS 0.4g and dissolve it in 11mL H 2 O, then add 2.2 g of the Gel-MA, and dissolve completely in a water bath at 50°C.

[0046] The inorganic bioceramic particle part (hydroxyapatite) was sieved twice with a standard sieve of 0.15 mm aperture, 3 g of the sieved particles were taken, and the inorganic bioceramic particles were added to the above-mentioned homogeneous solution in five times, after each addition, Put it into a planetary centrifugal mixer and mix for about 15 minutes. After the last 3g is completely added, mix for 1 hour to obtain a uniform and stable extruded slurry.

[0047] Transfer the slurry to the barrel of the extrusion bioprinter, match the needle with the aperture of 0.41mm, set the air pressure to about 0.4MPa, the line speed to 8mm / s, the line spacing to 1.1mm, set the platform temperature to 4°C, and the barrel temperature to 30°C , to print. After printing...

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Abstract

The invention discloses a biological material, a preparation method thereof and application of the biological material as a bone defect repairing material and belongs to the technical field of medicalbiological materials. The preparation method comprises the steps of adding a photoinitiator solution into an aqueous solution containing methacrylic anhydride gelatin, wherein the photoinitiator solution is a photoinitiator 819 solution or a mixed solution of the photoinitiator 819 and a photoinitiator 184; carrying out extrusion type 3D biological printing on printing slurry, and carrying out ultraviolet irradiation on the slurry when the slurry is extruded; preferably, the aqueous solution containing methacrylic anhydride gelatin also contains carboxymethyl chitosan; preferably, chemical crosslinking is carried out after printing is finished; preferably, an inorganic bioceramic material is added to the printing slurry. The method has the advantages of simple components, stable printingprocess, high precision, high crosslinking degree, high crosslinking speed and good scaffold biocompatibility, and the biological material can also be used as a carrier of medicines or other functional components, and has very wide application prospect in the field of bone defect repair.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and more specifically relates to a biomaterial, a preparation method thereof, and an application as a bone defect repair material. Background technique [0002] Bone damage due to accidents, aging, etc. often requires the implantation of bone repair materials during surgical treatment. However, autologous bone transplantation will cause secondary trauma, and allogeneic bone transplantation may cause immune reactions. The research on synthetic bone defect repair materials is very important and has a wide range of clinical application needs. Bone defect repair materials often use inorganic bioceramic particles (such as hydroxyapatite, bioactive glass, etc.) and polymers (gelatin, collagen, polylactic acid, etc.) to make porous scaffold materials. The ability of tissue to grow into and achieve osseointegration can also be degraded after bone regeneration to achieve new bone replacement...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/22A61L27/20A61L27/10A61L27/12A61L27/50A61L27/54C08J3/24C08J3/28B33Y70/00B33Y80/00C08L89/00C08L5/08
Inventor 马军石玉飞
Owner HUAZHONG UNIV OF SCI & TECH
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