Immune potentiator based on hirsutella sinensis and preparation method thereof
A technology of Mortierella sinensis and immune enhancer, applied in the field of immune enhancer and preparation based on Mortierella sinensis, to achieve the effect of increasing the content of immune active substances, high safety, and improving human immunity
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Embodiment 1
[0038] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:
[0039] 1) Strain selection
[0040] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );
[0041] 2) Strain culture
[0042] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:
[0043] 2.1) Preparation of primary solid bacteria
[0044]The isolated and purified slant strains were inoculated on the solid medium of a 9 cm petri dish, the medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 Add 3.0 g of agar, 20.0 g of agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in an incubator at 17°C for 20 days to obtain a first-grade solid strain.
[0045] ) secondary liquid seed culture
[0046] Put 200ml of liquid medium in a 500mL conical flask; each 10...
Embodiment 2
[0055] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:
[0056] 1) Strain selection
[0057] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );
[0058] 2) Strain culture
[0059] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:
[0060] 2.1) Preparation of primary solid bacteria
[0061] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish. The medium was: 200.0 g potato juice, 20.0 g glucose, 10.0 g maltose, 10.0 g peptone, 10.0 g yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH; put it in a 20°C incubator, and cultivate it for 30 days to obtain a first-class solid strain.
[0062] ) secondary liquid shake flask seed culture
[0063] Put 200ml of liquid medium in a 500mL Erlenmeyer flas...
Embodiment 3
[0073] The preparation operation steps of a kind of immune enhancer based on Mortierella sinensis are as follows:
[0074] 1) Strain selection
[0075] Cordyceps sinensis collected from Qinghai [ Ophiocordyceps sinensis 〕 Sinina isolated from the subunit ( Hirsutella sinensis );
[0076] 2) Strain culture
[0077] The culture method is liquid-solid mixed culture, and the specific procedures are as follows:
[0078] 2.1) Preparation of primary solid bacteria
[0079] The isolated and purified strains were inoculated on the solid medium of a 9 cm petri dish (the medium was: 200.0 g of potato juice, 20.0 g of glucose, 10.0 g of maltose, 10.0 g of peptone, 10.0 g of yeast powder, MgSO 4 1.5 g, KH2PO 4 3.0 g, 20.0 g agar, add water to 1.0 L, adjust the pH to 6.5 with 1.0 M NaOH), put them in an incubator at 18.5 ° C, and cultivate them for 25 days to obtain a first-class solid strain.
[0080] ) secondary liquid shake flask seed culture
[0081] Put 200ml of liquid mediu...
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