Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Derivative of 3-(3-hydroxylphenyl)-3-hydroxy-propionic acid, homogeneous enzyme immunodetection reagent and preparation method therefor

A technology of hydroxypropionase and homogeneous enzyme immunization, which can be used in the preparation of carboxylate, the preparation of organic compounds, the preparation of carboxylate/lactone, etc., and can solve the problems of complicated operation and the like

Active Publication Date: 2019-05-03
苏州博源医疗科技有限公司
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the commonly used methods for detecting HPHPA are gas chromatography-mass spectrometry (GC-MS) and enzyme-linked immunoassay (ELISA), etc., but these methods are complicated to operate and have certain limitations in large-scale clinical application.
At present, there is a lack of HPHPA detection reagents with good stability, high sensitivity and strong specificity in the market, especially high-throughput automated detection reagents with good quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Derivative of 3-(3-hydroxylphenyl)-3-hydroxy-propionic acid, homogeneous enzyme immunodetection reagent and preparation method therefor
  • Derivative of 3-(3-hydroxylphenyl)-3-hydroxy-propionic acid, homogeneous enzyme immunodetection reagent and preparation method therefor
  • Derivative of 3-(3-hydroxylphenyl)-3-hydroxy-propionic acid, homogeneous enzyme immunodetection reagent and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1. Synthesis of 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative

[0079] The synthetic route of 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative is as follows:

[0080]

[0081] The preparation process of 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative is as follows:

[0082] (1) Synthesis of compound 3:

[0083] Weigh 20g of compound 1, namely 3-(3-hydroxyphenyl)-3-hydroxypropionic acid (purchased from Sigma) and 33.2g of compound 2 (purchased from Sigma), and dissolve them in 300mL DMF together. Add 36.4g of K 2 CO 3 , The reaction mixture solution was prepared, and the above reaction mixture solution was stirred at room temperature for 12 hours. After the reaction was completed, 200 mL of purified water was added to the above reaction solution, and the solution was extracted with 300 mL of DCM. The extraction step was repeated 3 times. The organic phase obtained by extraction is passed through Na 2 SO 4 After drying, the residue obtained after co...

Embodiment 2

[0086] Example 2. Synthesis of 3-(3-hydroxyphenyl)-3-hydroxypropionic acid immunogen

[0087] The 3-(3-hydroxyphenyl)-3-hydroxypropionic acid immunogen in this example is formed by linking the 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative obtained in Example 1 with BSA, Its structural formula is shown in the following formula (II):

[0088]

[0089] The carrier is BSA.

[0090] The specific steps of the synthesis method of the 3-(3-hydroxyphenyl)-3-hydroxypropionic acid immunogen are as follows:

[0091] (1) Weigh 2.72g potassium dihydrogen phosphate, 4.26g disodium hydrogen phosphate, 8.5g sodium chloride, 0.95g magnesium chloride, and dissolve them in 1L of deionized water, adjust the pH to 8.2, and prepare buffer A;

[0092] (2) Weigh 3 mg of BSA and dissolve it in 3 mL of the above buffer A at -4°C to make a 1 mg / mL carrier solution;

[0093] (3) Weigh 3 mg of the 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative obtained in Example 1, and dissolve it in 300 μl of the ...

Embodiment 3

[0096] Example 3. Synthesis of 3-(3-hydroxyphenyl)-3-hydroxypropionic acid immunogen

[0097] The 3-(3-hydroxyphenyl)-3-hydroxypropionic acid immunogen in this example is obtained by linking the 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative obtained in Example 1 with ovalbumin. The structural formula is as shown in the following formula (Ⅱ)

[0098]

[0099] The carrier is ovalbumin.

[0100] The specific steps of the synthesis method of the 3-(3-hydroxyphenyl)-3-hydroxypropionic acid immunogen are as follows:

[0101] (1) Weigh 2.3g of potassium dihydrogen phosphate, 3.8g of disodium hydrogen phosphate, 8.0g of sodium chloride, and 0.7g of magnesium chloride, and dissolve them in 0.9L of deionized water, adjust the pH to 8.0, and prepare buffer A;

[0102] (2) Weigh 6 mg of ovalbumin and dissolve it in 3 mL of buffer A at -4°C to make a 2 mg / mL carrier solution;

[0103] (3) Weigh 2.4 mg of the 3-(3-hydroxyphenyl)-3-hydroxypropionic acid derivative obtained in Example 1, and d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention discloses a derivative of 3-(3-hydroxylphenyl)-3-hydroxy-propionic acid (HPHPA), a homogeneous enzyme immunodetection reagent and a preparation method therefor and relates to the technical field of biological detection. An HPHPA immunogen with high immunogenicity and an antibody thereof are prepared by using the derivative of the HPHPA, and the antibody is high in specificity and high in potency and is free of any cross reaction with 92 kinds of common interfering substances; and the HPHPA homogeneous enzyme immunodetection reagent prepared by using the antibody can achieve high-flux and rapid detection on the HPHPA on a fully-automatic biochemical analyzer, the accuracy of detection is high, the specificity is high, and the efficiency of detection is remarkably increased.

Description

Technical field [0001] The invention relates to the technical field of biological detection, in particular to derivatives of 3-(3-hydroxyphenyl)-3-hydroxypropionic acid, homogeneous enzyme immunoassay reagents and preparation methods thereof, and more particularly to a 3-(3- (Hydroxyphenyl)-3-hydroxypropionic acid derivatives, immunogens, antibodies, enzyme-labeled conjugates, detection reagents and preparation methods thereof. Background technique [0002] 3-(3-Hydroxyphenyl)-3-hydroxypropionic acid (3-(3-Hydroxyphenyl)-3-hydroxypropionicAcid, HPHPA), its structural formula is as shown in formula (IV): [0003] [0004] HPHPA is a metabolite of 3-hydroxyphenylalanine (tyrosine analogue) and a metabolite of Clostridium spp. It is excreted in urine. It can cause stereotyped behaviors, hyperactive and highly reactive autism-like behaviors by depleting catecholamines in the brain. [0005] Autism spectrum disorders (ASD), also known as generalized developmental disorders or autism, ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C59/68C07C51/09C07C69/712C07C67/31C07K14/765C07K14/77C07K14/795C07K14/47C07K16/44
Inventor 虞留明王益超娄明星
Owner 苏州博源医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com