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Honeybee pupae extract and application in protecting against cisplatin induced kidney injury

An extract, a technology for kidney injury, which is applied to bee pupae extract and the application field in protecting cisplatin-induced kidney injury, can solve the problems of combined use of cisplatin, intensive use of bee pupae for medicinal or protective effects, etc. The effect of enhancing resistance, reducing nephrotoxicity, and improving animal physique

Active Publication Date: 2019-04-26
普洱学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In summary, there is currently no material that can be used in combination with cisplatin to reduce its toxic and side effects and better exert its effects in the prior art; at the same time, there is no research on the medicinal or protective effects of bee pupae in the use of bee pupae. in-depth technical innovation

Method used

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  • Honeybee pupae extract and application in protecting against cisplatin induced kidney injury
  • Honeybee pupae extract and application in protecting against cisplatin induced kidney injury
  • Honeybee pupae extract and application in protecting against cisplatin induced kidney injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of bee pupa extract, comprising the following steps:

[0026] Step 1. Use a syringe to absorb the bee pupa tissue liquid, homogenize it with a glass homogenizer, apply a voltage of 150V, ultrasonically disrupt the cells for 30 minutes, centrifuge at 12000 rpm / min for 10 minutes, and take the supernatant;

[0027] Step 2. According to the ratio of petroleum ether: bee pupa = 1:2, mix on a vortex oscillator for 3-5 minutes, centrifuge at 12000 rpm / min for 5 minutes, and repeat this step until completely degreased;

[0028] Step 3: Make freeze-dried powder in a vacuum freeze dryer, pack in centrifuge tubes, seal tightly with parafilm, and store in a -80°C refrigerator.

[0029] Both step 1 and step 2 were performed on ice.

[0030] The bee pupa extract used in this experimental study was obtained from wild wasp larvae.

Embodiment 2

[0032] Application of honeybee pupae extract in protecting kidney injury induced by cisplatin

[0033] Preparation of mouse model of cisplatin-induced kidney injury

[0034] Select 18 healthy male mice and randomly divide them into blank control group, cisplatin (CDDP) group and bee pupae group, with 6 mice in each group;

[0035] The control group and the CDDP group were given 0.3mL 0.9% normal saline, once a day, for 15 days in total;

[0036] The bee chrysalis group was given the bee chrysalis extract (the bee chrysalis extract was prepared with 0.9% physiological saline) at a dose of 200 mg / kg, once a day, for 15 consecutive days;

[0037] From the 11th day of gavage, the CDDP group and the bee pupae group were injected with 2 mg / kg cisplatin solution (cisplatin was prepared with 0.9% normal saline), once a day for 5 consecutive days, and the control group was injected with 0.9% normal saline. During this period, the mice were fed with basal feed, free to eat and drink w...

Embodiment 3

[0058] Application of bee chrysalis extract in the preparation of a drug that protects the kidney damage induced by cisplatin in mice;

[0059] A medicine for treating kidney damage caused by cisplatin, comprising the honeybee pupa extract;

[0060] Based on the above studies, bee pupae extract has extremely high free radical scavenging activity, can improve the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the body, and inhibit cisplatin The molecular mechanism of inducing the formation of superoxide free radicals, and from the role of reducing its nephrotoxicity, the research on the use of bee pupa extracts for the treatment of cisplatin-induced kidney injury or the preparation of cisplatin-induced mouse kidney injury protection Drugs that work.

[0061] The present invention firstly prepares the bee pupa extract, and applies it to the treatment of cisplatin-induced kidney damage, and has the effect of treating the kidney damage caused by cis...

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Abstract

The invention discloses a honeybee pupae extract and application in protecting against cisplatin induced kidney injury, and relates to the technical field of honeybee pupae and application. Accordingto the invention, the honeybee pupae extract is firstly prepared and then a product of the honeybee pupae extract is applied to treat the cisplatin induced kidney injury, and has the effects of treating the cisplatin induced kidney injury. An experiment finds that the honeybee pupae extract has extremely-high free radical scavenging activity and can improve the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in an organism. By inhibiting a molecular mechanism of inducing by cisplatin to form superoxide free radicals, the effect of reducing renal toxicity is achieved; meanwhile, the honeybee pupae extract has extremely-high nutritional value, so that animal physique can be improved and the immunity is enhanced; in addition, because the honeybee pupae extract isderived from active ingredients in the organism, the honeybee pupae extract has medicine and food dual-purpose function, and no toxic or side effects, can be obtained through ingestion and is expectedto be further developed as a safe and reliable treatment drug.

Description

technical field [0001] The invention relates to the technical field of bee pupae and its application, in particular to a bee pupae extract and its application in protecting cisplatin-induced kidney damage. Background technique [0002] Cisplatin (cis-dichlorodiammine platinum Ⅱ, Cisplatin, CDDP) is one of the commonly used anticancer drugs in clinical practice. Cisplatin has the characteristics of strong anti-cancer effect, high toxicity and side effects, synergistic effect with various anti-tumor drugs, and lack of cross-drug resistance. After injection of cisplatin, cisplatin will reach the organs and tissues of the whole body along with the blood circulation, mainly appearing in the liver, kidney, ovary, skin, uterus and so on. Cisplatin-induced nephrotoxicity mainly involves multiple mechanisms such as cytotoxicity, oxidative stress, apoptosis, and inflammatory response. Clinically, the curative effect of cisplatin is directly proportional to its dose. The greater the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P13/12
CPCA61P13/12A61K35/64
Inventor 赵锋杨绍伟赵芳熊萧然冯玄
Owner 普洱学院
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