Fluorescent probe for tumor diagnosis and treatment as well as preparation method and application of fluorescent probe

Abstract

The invention provides a fluorescent probe for tumor diagnosis and treatment as well as a preparation method and an application of the fluorescent probe. In the fluorescent probe, an azacyclo structure base is taken as an H<+> receptor, a near infrared fluorescent dye with high extinction coefficient and tumor targeting effect is taken as a fluorophore, then the OFF-ON type probe capable of regulating fluorescence intensity by rapid PET (photoinduced electron transfer) process is formed, fluorescence of the probe is strengthened in a slightly acidic environment of tumor, tumor detection is realized, the probe has strong PA (photoacoustic) signals at the tumor while fluorescence imaging is performed, and tumor boundary can be determined accurately by NIRF / PA bimodal imaging. The fluorescentprobe also has good photo-thermal treatment effect on the tumor and can be used for preparing corresponding tumor treatment drugs.

Description

technical field [0001] The present invention relates to the field of drugs for tumor diagnosis and treatment, in particular to a fluorescent probe for tumor diagnosis and treatment as well as its preparation method and application. Background technique [0002] Cancer is one of the biggest killers threatening human life around the world, and it is also a major challenge in the field of current medical research. How to effectively prevent and treat cancer has become a top priority in scientific research. [0003] Clinically, cancer is mainly treated by three methods: surgery, radiation therapy and chemotherapy. However, surgical treatment has defects such as high risk, large trauma, and prone to complications; while radiotherapy and chemotherapy kill cancer cells, they will also cause serious damage to normal cells in the human body, greatly reducing the survival of patients. quality. Therefore, the development of efficient and low-toxic treatment methods is of great signi...

AI Technical Summary

Problems solved by technology

[0006] However, if you want to use nanosystems for clinical diagnosis and treatment in a safer, more reliable, efficient and low-toxicity way, you need to conduct in-depth exploration of many basic issues in nanosystems to overcome the bottleneck problems faced by common nanomaterials, such as: quantum Nano-gold / silver particles are inorganic heavy metal materials, which are easy to accumulate in living organisms and difficult to degrade; nano-gold / silver particles have a great impact on normal cell activities and metabolism in vivo, and the particle size and shape are greatly affected by the environment, which greatly reduces Its application in the field of tumor diagnosis and treatment; the distribution of nano-carbon materials in the body is complex and difficult to degrade, and there is potential biotoxicity; Accurate positioning and stability after combining with drug carriers have a great influence; the size of lipid / polymer nanoparticles is difficult to control, easy to metabolize and degrade in vivo, and the tumor targeting effect is poor; the metabolic ability of mesoporous nanomaterials is relatively low. Poor, and the interaction mechanism between the loaded diagnosis and treatment drugs and mesoporous materials is difficult to analyze, and the impact on the diagnosis and treatment process has not yet been comprehensively and in-depth research

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