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Composite scaffold for loading bone marrow mesenchymal stem cells and preparation method thereof

A technology of bone marrow mesenchymal and composite scaffolds, which is applied in the field of composite scaffolds loaded with bone marrow mesenchymal stem cells and its preparation, and can solve problems such as no occurrence

Active Publication Date: 2019-02-01
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after traditional stem cells leave the human body, they will undergo apoptosis in a short period of time due to the lack of a suitable living environment. Therefore, it is necessary to find suitable materials to load stem cells, so that stem cells can survive for a long time; and in the treatment of certain diseases (such as steroid-induced femoral head failure), the material needs to be transplanted into a high-pressure internal environment, which makes the material have a strong elastic modulus, but there is no such thing on the market that can make stem cells survive for a long time Biomaterials with large elastic modulus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of modified graphene

[0034] Described modified graphene comprises following composition by weight:

[0035] 100 parts of graphene;

[0036] 4 parts of 3-glycidyl etheroxypropyl triethoxysilane;

[0037] 2 parts of phenyl tris(dimethylsiloxane) silane.

[0038] 3 parts of 3-methacryloxypropylmethyldimethoxysilane.

[0039] The preparation method of described modified graphene is:

[0040] Step 1: Weigh the required substances according to the set weight parts; graphene oxide produced by oxidizing graphene;

[0041] Step 2: Add 3-glycidyloxypropyltriethoxysilane, phenyltris(dimethylsiloxane)silane and 3-methacryloxypropylmethyldimethoxysilane Dispersed in acetone solution;

[0042] Step 3: Add graphene oxide to the acetone solution obtained in step 2, mix evenly, and react at 70° C. for 12 hours to obtain modified graphene.

[0043] The first step is to add excess concentrated sulfuric acid into the container, control the temperature...

Embodiment 2

[0044] Embodiment 2: the preparation of hydroxyapatite

[0045] S1: Dissolve calcium nitrate in deionized water to form solution A; dissolve sodium dihydrogen phosphate in absolute ethanol to form solution B; the concentrations of solution A and solution B are both 1mol / L; after preparation, use The concentration is 0.1mol / L ammonia water to adjust the pH of solution A and solution B to 10;

[0046] S2: Put 16ml of solution A and 10ml of solution B into the reactor and stir and mix them at a speed of 150rad / min for 10min to form a mixed solution. Add 3g of PEG-400 to the mixed solution, and then set the temperature of the reactor to 50°C. Mix and stir for 4 hours, while maintaining the pH of the mixed solution at 10 with ammonia water;

[0047] S3: Place the mixed solution in a water bath at 55°C for 24 hours to obtain a white colloidal liquid; filter it after ultrasonic oscillation at room temperature for 30 minutes, and wash it with absolute ethanol three times to obtain a sa...

Embodiment 3

[0049] Embodiment 3: the preparation of composite support

[0050] A composite scaffold for loading bone marrow mesenchymal stem cells, comprising the following components by weight:

[0051] 100 parts of methacrylated gelatin;

[0052] 8 parts of modified graphene;

[0053] 8 parts of hydroxyapatite;

[0054] 4 parts blending agent;

[0055] 6 parts of modified additives.

[0056] The blending agent is a mixture of 1-(3-pyridyl)-3-(dimethylamino)-2-propen-1-one and resorcinol diglycidyl ether, the mass ratio of which is 1:1.

[0057] The modification additives are 4-acetylamino-2,2,6,6-tetramethyl-1-piperidinyloxy, ethyltrimethylsilyltrimethylsilylketene, N,N' - A mixture of bis(chlorodimethylsilyl)-1,1-dimethyl-silanediamine and 1,5-pentanediol diacrylate in a mass ratio of 3:2:2:5.

[0058] A method for preparing a composite scaffold for loading bone marrow mesenchymal stem cells, comprising the following steps:

[0059] Step 1: prepare raw materials according to the...

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Abstract

The invention discloses a composite scaffold for loading bone marrow mesenchymal stem cells and a preparation method thereof. Hydroxyapatite and modified graphene are added into methacrylated gelatin,and for improving synergy, a blender and a modifying auxiliary agent are added; a mixture of 1-(3-pyridyl)-3-(dimethylamino)-2-propylene-1-one and diglycidyl resorcinol ether is selected as the blender, and a mixture of 4-(acetylamino)-2,2,6,6-tetramethyl-1-piperidinyloxy, trimethylsilylketene ethyl trimethylsilyl acetal, N,N'-di(chlorodimethylsilane)-1,1-dimethylsilane diamine and 1,5-pentanediol diacrylate is selected as the modifying auxiliary agent. The finally prepared composite scaffold can make the stem cells survive longer, also has large elastic modulus. In addition, due to the addition of modified graphene and hydroxyapatite, the composite scaffold has good biocompatibility and is not easily rejected by a human body.

Description

technical field [0001] The invention relates to the field of biological materials, and more specifically relates to a composite scaffold for loading bone marrow mesenchymal stem cells and a preparation method thereof. Background technique [0002] With the rapid development of science and technology, regenerative medicine represented by cell therapy has gradually become an important direction for the development of clinical medicine, bringing new hope for complex and serious diseases that are difficult to be effective in drug treatment. Cell therapy is a treatment method that uses the patient's own (or allogeneic) adult cells or stem cells to repair tissues and organs. However, after traditional stem cells leave the human body, they will undergo apoptosis in a short period of time due to the lack of a suitable living environment. Therefore, it is necessary to find suitable materials to load stem cells, so that stem cells can survive for a long time; and in the treatment of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/44A61L27/46A61L27/50A61L27/54C01B32/194A01N1/02C01B25/32
CPCA01N1/0263A61L27/3834A61L27/446A61L27/46A61L27/50A61L27/54A61L2300/412A61L2400/06C01B25/325C01B32/194C08L89/00
Inventor 陈春陈雷林垂聪
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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