Method for identifying wool species of ancient wool fabric fragments based on immunoblotting

Abstract

The invention relates to the field of cultural relic detection, and discloses a method for identifying the wool species of ancient wool fabric fragments based on immunoblotting. The method comprises:preparing carbon quantum dots by using a reverse micro-emulsion method; labeling goat wool secondary antibody and sheep wool secondary antibody with the carbon quantum dots; extracting modern wool andan ancient wool fabric fragment by using a metal salt method and a reduction method, carrying out dialysis, purifying, carrying out SDS-PAGE gel electrophoresis, staining with a carbon quantum dot solution, and observing the electrophoresis image; transferring the obtained protein bands to a polyvinylidene fluoride membrane; and incubating with a wool primary antibody and a carbon quantum dot-labeled secondary antibody, observing the immunological bands in a gel imaging system, and identifying the wool species of the ancient wool fabric. According to the present invention, the method has advantages of less consumption of the chemical reagent, mild reaction, environmental protection and no harm; and the method further has advantages of less sample consumption, sensitivity, accuracy, intuitiveness and the like in the detection of ancient wool fabrics.

Description

technical field [0001] The invention relates to the field of detection of cultural relics, in particular to a method for identifying wool types of ancient wool fabric fragments based on the immunoblotting method. Background technique [0002] China has a long history. A large number of wool products have been unearthed from early Chinese tombs. The quantity and the exquisite weaving are both amazing. Studying the wool types of these unearthed woolen fabrics can help us better understand the origin of ancient woolen fabrics, ethnic minority costumes, and cultural exchanges between ethnic groups. [0003] Among the remains of ancient wool fabrics, wool remains are mainly wool fabric fragments. Such remains have been buried in the underground environment for a long time. Under the influence of environmental factors such as water, heat, oxygen, and microorganisms, wool keratin will undergo denaturation and aging degradation. After being unearthed, due to drastic changes in en...

AI Technical Summary

Problems solved by technology

After being unearthed, due to drastic changes in environmental conditions, the aging of fibers was further accelerated. Therefore, it is not difficult to imagine that in the long history, woolen fabrics buried in tombs or ruins have already lost their original appearance and degraded into Peptides or amino acids cannot be recognized by the naked eye, and the earlier the evidence is, the harder it is to find, which brings great difficulties to the identification of wool types in woolen cultural relics

[0004] At present, ordinary analysis and detection techniques, such as Fourier transform infrared spectroscopy, Raman spectroscopy, X-ray diffraction analysis, etc., are often at a loss when encountering such residue analysis. Most of the identification of ancient woolen fabrics at home and abroad still remain In the analysis of fiber morphology, it is difficult to identify the wool species of different ancient woolen fabrics; therefore, it is very necessary to find a more scientific method to identify ancient woolen fabrics