Method for modifying nano-decalcification bone matrix particle coating layer by virtue of polycaprolactone-tricalcium phosphate bone tissue engineering scaffold

A technology of tissue engineering scaffold and decalcified bone matrix, which is applied in the fields of coating, tissue regeneration, and pharmaceutical formulations to achieve good plasticity, meet clinical needs, and have a wide range of sources

Active Publication Date: 2019-01-11
SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The organic combination of the three has not yet been reported in the literature

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  • Method for modifying nano-decalcification bone matrix particle coating layer by virtue of polycaprolactone-tricalcium phosphate bone tissue engineering scaffold
  • Method for modifying nano-decalcification bone matrix particle coating layer by virtue of polycaprolactone-tricalcium phosphate bone tissue engineering scaffold
  • Method for modifying nano-decalcification bone matrix particle coating layer by virtue of polycaprolactone-tricalcium phosphate bone tissue engineering scaffold

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Experimental program
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Embodiment 1

[0053] A method for modifying a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold with a nano-decalcified bone matrix particle coating, comprising the following steps:

[0054] 1. The allogeneic nano-decalcified bone matrix (DBM) material is mixed with 75% ethanol to dilute the nano-pulp

[0055] Mix the allogeneic nano-decalcified bone matrix (DBM) material (prepared in Example 3) with 75% ethanol at a volume ratio of 1:5, and oscillate sufficiently to evenly distribute the nano-DBM particles in the ethanol.

[0056] The mixing conditions are as follows: 20-25°C at room temperature, instrument: NHWY-100B tabletop constant temperature shaker, temperature control range is 5-60°C (30°C constant temperature), speed 40-300rmp, amplitude range Ф26mm, Changzhou Nuoji Instrument Co., Ltd. Company, the time is 5 minutes.

[0057] 2. Preliminary Freezing

[0058] Soak the PCL-TCP bone tissue engineering scaffold (20-25°C at room temperature, soak for 2 minutes) i...

Embodiment 2

[0069] A method for modifying a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold with a nano-decalcified bone matrix particle coating, comprising the following steps:

[0070] 1. The allogeneic nano-decalcified bone matrix (DBM) material is mixed with 75% ethanol to dilute the nano-pulp

Embodiment 3

[0074] The preparation method of adult allograft nanometer decalcified bone matrix material comprises the following steps:

[0075] 1. The fresh adult allogeneic bone was removed from the soft tissue, and the modified Urist method was used to prepare massive nano-decalcified bone matrix (DBM) bone blocks

[0076] (1) Source of bone tissue

[0077] The allogeneic bone used for bone tissue decalcification comes from the femoral head, tibial plateau, and femoral condyle resected from patients undergoing hip and knee replacement in joint surgery from 2015 to 2016 in Changzheng Hospital Affiliated to Naval Military Medical University. Approved by the Medical Ethics Committee of Changzheng Hospital, it was approved to be used in clinical research. The selected donors all meet the following requirements of the American Tissue Bank Association: no history of acute and chronic infectious diseases; no history of exposure to toxic substances and drug abuse; no history of tumors, sexuall...

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Abstract

The invention discloses a method for modifying a nano-decalcification bone matrix particle coating layer by virtue of a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold. The method comprises the following steps: mixing an allogeneic decalcification bone matrix material with 75% ethanol, soaking the polycaprolactone-tricalcium phosphate bone tissue engineering scaffold into diluted nano-decalcification bone matrix slurry, putting the polycaprolactone-tricalcium phosphate bone tissue engineering scaffold into liquid nitrogen, so as to finish primary freezing; putting the obtained frozen scaffold into a vacuum freeze-drying machine, carrying out freeze-drying for at least 48 hours to finish the modification of a surface coating layer, so as to obtain the polycaprolactone-tricalcium phosphate bone tissue engineering scaffold modified with the nano-decalcification bone matrix particle coating layer. By utilizing a low-temperature freezing method, the activity of a nano-DBM biological material is effectively guaranteed; and the osteogenic capability of the scaffold is improved based on original scaffold holes. According to the method, a certain theoretical basis isprovided for the research and development of bone transplantation substitutions and bone defect repairing materials.

Description

technical field [0001] The invention relates to the technical field of bone tissue engineering, in particular to a method for modifying a polycaprolactone-tricalcium phosphate bone tissue engineering scaffold to coat nanometer decalcified bone matrix particles. Background technique [0002] The repair of bone defects has always been a difficult and hot topic in the field of orthopedics. At present, bone defects are usually filled with autologous bone, allogeneic bone or tissue engineered bone. As the gold standard for bone defect repair, autologous bone has ideal properties of induction, conduction, and osteogenesis. However, its source is limited, and there are complications such as donor site infection and chronic pain. Although allograft bone comes from a wide range of sources and has good osteogenic and conductive osteogenic abilities, the source control is limited, and there are risks such as pathogen transmission and immune rejection. At present, more scholars focus ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/12A61L27/28
CPCA61L27/12A61L27/18A61L27/28A61L2400/18A61L2420/02A61L2430/02C08L67/04
Inventor 陈雄生王智巍苑博周盛源唐一钒许国峰
Owner SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV
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