Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for simultaneously determining contents of two active ingredients in Salvia miltiorrhiza-pseudo-ginseng preparation

A preparation, the technology of notoginseng, applied in the field of medicine, can solve the problems of effective quality control, time-consuming, and inability to realize prescriptions, and achieve the effect of fast method and improved work efficiency

Inactive Publication Date: 2018-11-30
BEIJING RED SUN PHARMA
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The quality control of Danqi preparations in the prior art only controls the components of notoginseng saponins. For example, Danqi Tablets (Chinese Pharmacopoeia 2015 Edition Part One, page 676) only controls notoginsenoside R1, ginsenoside Rg1 and ginsenoside Re These methods are all one-sided to a certain extent, and cannot effectively control the overall quality of the prescription; there are also methods that control danshensu and notoginsenoside R1, ginsenoside Rg1 and ginsenoside Re, but due to the combination of danshensu and three The chemical structure of heptasaponins is quite different, and the detection wavelengths of the two substances are quite different when they are detected by chromatography, and they cannot be detected at the same wavelength. page), the two types of substances need to be detected by two different chromatographic methods, and the quality control steps are cumbersome and time-consuming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously determining contents of two active ingredients in Salvia miltiorrhiza-pseudo-ginseng preparation
  • Method for simultaneously determining contents of two active ingredients in Salvia miltiorrhiza-pseudo-ginseng preparation
  • Method for simultaneously determining contents of two active ingredients in Salvia miltiorrhiza-pseudo-ginseng preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Detection of the content of two types of four active ingredients in Danqi Soft Capsules

[0024] (1) Instrument

[0025] Ultra-high performance liquid chromatography (UPLC, Waters, USA); series API 5500 triple quadrupole mass spectrometer (Applied Biosystems, USA), equipped with Waters UPLC autosampler, column thermostat, and data acquisition was completed by Analyst software.

[0026] (2) Instrument operating conditions

[0027] Liquid chromatography conditions: Waters ACQUITY UPLC BEH C18 column (2.1mm×50mm, 1.7μm); injection volume is 1μL; flow rate is 0.4mL / min; column temperature is 30°C; mobile phase A has a volume ratio of 0.1: 99.9 mixed solution of formic acid and water, mobile phase B is a mixed solution of formic acid and acetonitrile with a volume ratio of 0.1:99.9; carry out gradient elution, the gradient conditions are:

[0028]

[0029] Mass spectrometry conditions: ion source is Turbo V; ionization mode is ESI - The air curtain gas volum...

Embodiment 2

[0045] Example 2 Determination of Dissolution Rates of Two Kinds of Four Active Components of Danqi Soft Capsules

[0046] (1) Conditions and determination of dissolution rate

[0047] Measure 900ml of degassed dissolution medium (1% SDS aqueous solution), heat the medium temperature up to (37±0.5)°C, take 6 Danqi soft capsules, 6 Danqi tablets and 6 Danqi tablets Seven capsules, 1 capsule in each basket, 6 repetitions of each preparation, test at 100r / min, 2mL samples were taken at fixed points at 1, 5, 10, 20, 30, 45, 60, 70, 90min, At the same time, add the same medium at the same temperature and the same amount, add methanol to the sample to 4mL, shake well to pass through a 0.22μm PTFE filter membrane, take the subsequent filtrate, inject 1μL by UPLC-TQ MS method, measure the content of the target compound, and calculate the target compound cumulative dissolution rate. Taking time as the abscissa and the cumulative dissolution rate as the ordinate, the dissolution curve...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of medicines, and particularly relates to a method for simultaneously determining contents of two active ingredients in a Salvia miltiorrhiza-pseudo-ginseng preparation. The method uses UPLC-TQ MS to simultaneously determine the four active components of Danshensu, Salvianolic acid A, Notoginsenoside Re and Notoginsenoside R1 in the Salvia miltiorrhiza-pseudo-ginseng preparation. The method is proved to be fast, stable and reliable, and can provide an important basis for the quality evaluation of the Salvia miltiorrhiza-pseudo-ginseng preparation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for simultaneously measuring the contents of two types of active ingredients in Danqi preparations. Background technique [0002] The whole prescription of Danqi Preparation is composed of Danshen and Panax notoginseng. It has the functions of dispelling blood stasis and hemostasis, reducing swelling and relieving pain; the combined use of the two medicines has the effect of promoting blood circulation, dissipating blood stasis, reducing swelling and relieving pain. Clinically, it is mostly used for diseases caused by congestion, such as: chest obstruction and heartache, congestion headache, bruises, irregular menstruation, lower abdominal pain caused by postpartum stasis, traumatic hematoma, dysmenorrhea, lochia, coronary heart disease, angina pectoris, high blood pressure etc. In addition to the earliest Danqi tablets, the types of Danqi preparations als...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 林德良杨健
Owner BEIJING RED SUN PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com