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SNP molecular marker related to quantity of multiparity piglets of pigs, identification and application thereof

A litter size and long-term production technology, applied in the field of pig breeding, can solve problems such as lack of understanding and understanding, incomplete grasp of genes, etc., and achieve the effect of improving the theoretical level and technical level

Inactive Publication Date: 2018-11-27
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current understanding and understanding of the pig genome is far from enough, and the genes that affect the reproductive efficiency of pigs are not fully grasped. Further research is necessary to meet the needs of pig breeding methods

Method used

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  • SNP molecular marker related to quantity of multiparity piglets of pigs, identification and application thereof
  • SNP molecular marker related to quantity of multiparity piglets of pigs, identification and application thereof
  • SNP molecular marker related to quantity of multiparity piglets of pigs, identification and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] The acquisition of embodiment 1 SNP molecular marker

[0103] 1. Experimental sample collection

[0104] 234 large white pigs from a pig farm in Shandong were used for SNP typing of DHRS4 gene. Collect ear tissue samples one by one, put them in 75% alcohol and store them at -20°C for DNA extraction.

[0105] 2. DHRS4 gene SNP typing

[0106] 2.1 Extraction and detection of pig genomic DNA

[0107] (1) Cut an appropriate amount of pig ear tissue, cut it into pieces and put it into a 1.5mL Axgen tube;

[0108] (2) Take a 50mL BD centrifuge tube, mix proteinase K with a final concentration of 0.4mg / mL and lysis buffer evenly, and add 0.5mL lysis solution to the 1.5mL centrifuge tube containing pig ear tissue for lysis;

[0109] (3) Place the centrifuge tubes in parallel and evenly on the shaking plate of the thermostatic hybridization furnace (seal the tube caps tightly to avoid liquid leakage). Place at 55°C for more than 6 hours (it is very important to fully mix th...

Embodiment 2

[0144] Example 2 Detection of DHRS4 gene expression

[0145] 1. Experimental sample collection

[0146] The large white pigs and Meishan pigs used to detect the expression of DHRS4 gene came from the pig farm of Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Wuqing District, Tianjin. The ovaries of sows at 180 days and 300 days after birth were collected, and the samples were stored in liquid nitrogen, and total RNA was extracted. Three individuals of each species were collected as biological replicates. Among them, Meishan pig is one of the breeds with the strongest fecundity and the largest litter size among pig breeds in my country, and its litter size is 30% to 40% higher than that of modern European pig breeds such as Large White pigs. Comparing the expression of DHRS4 gene between Meishan pig and Large White pig can effectively prove the effect of DHRS4 gene expression on litter size.

[0147] 2. Detection of D...

Embodiment 3

[0175] Example 3 Detection of HRS4 gene-related protein expression level indicators

[0176] 1. Tissue sample processing and total protein quantification

[0177] Tissue sample processing: Take out the ovarian tissue from liquid nitrogen, add 100μL RIPA lysate, and add DTT and PMSF at a final concentration of 1mM just before use. Grind the tissue with a grinding pestle, freeze and thaw several times in liquid nitrogen to fully lyse the cells; place on ice for 30 minutes, then centrifuge at 12000g for 30 minutes at 4°C, and collect the supernatant;

[0178] Total protein quantification: operate according to the instructions of the BCA protein quantification kit, the standard preparation is shown in Table 7, and 10 μL is added after the sample is diluted 10 times. Read values ​​at 570nm on a microplate reader. Draw the standard curve with A570 as the ordinate and the concentration of the standard as the abscissa. The concentration of the protein sample is obtained from the sta...

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Abstract

The invention discloses an SNP site related to the quantity of multiparity piglets of pigs. The SNP site is located on Chr7:75245594 of a pig reference genome Sscrofa11.1; the two equipotential genesof the SNP site are C and T; three genotypes corresponding to the SNP site are respectively CC, TT and TC; and compared with the pigs with genotypes of TT and TC, the pigs with genotypes of CC have higher quantity of multiparity piglets. The confirmation for the correlation between the SNP site and the quantity of multiparity piglets can effectively be used for identifying or assisting in identifying the quantity of multiparity piglets and can quicken the breeding process of the high-yield piglets.

Description

technical field [0001] The invention relates to the field of pig breeding, in particular to a SNP molecular marker related to pig litter size and its identification for improving pig reproduction efficiency. Background technique [0002] Reproductive performance is one of the key factors affecting the economic benefits of pig farming. As a perennial viviparous animal, a very important indicator of reproductive traits is litter size, especially the litter size. The litter size can be directly related to the number of fattening pigs and the amount of pork supply. [0003] The reproductive ability of sows is affected by many factors, including sow breed, parity, placental efficiency, hormone level, nutritional level, breeding work, etc. Among them, steroid hormones include adrenal cortex hormones and sex hormones (male hormones and estrogens), which play an important role in regulating the reproductive ability of sows and maintaining normal pregnancy. Recognition of the role ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11G01N33/573
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/158G01N33/573G01N2333/904
Inventor 杨亚岚李奎周荣刘颖李文通李华于辉
Owner FOSHAN UNIVERSITY
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