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Method for assaying glucosamine and chondroitin sulfate

A technology of chondroitin sulfate and glucosamine, which is applied in the field of analytical chemistry to achieve the effects of accurate results, strong specificity and not easy to be lost.

Active Publication Date: 2018-11-23
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing domestic and foreign standards, patents and papers do not solve the problem of determination of glucosamine and chondroitin sulfate well, so it is necessary to develop an analytical method with simple operation, high sensitivity, good repeatability and accurate results

Method used

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  • Method for assaying glucosamine and chondroitin sulfate
  • Method for assaying glucosamine and chondroitin sulfate
  • Method for assaying glucosamine and chondroitin sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The selection of embodiment 1 chromatographic conditions

[0070] Preparation of the reference solution: Weigh 5mg of chondroitin sulfate and D-glucosamine hydrochloric acid dried at 105°C for 4 hours in a 10ml measuring bottle, add 1ml of acetonitrile, oscillate to disperse evenly, then add water, sonicate to dissolve, water Dilute to the mark to obtain a mixed reference solution containing 0.5 mg each of glucosamine and chondroitin sulfate per 1 ml.

[0071] Preparation of the test solution: Take 20180201 batches of samples, remove the film-coated tablets, grind finely, take 100mg of powder, accurately weigh it, place it in a 50mL measuring bottle, add 5mL of acetonitrile, ultrasonically disperse for 1min, add water to the mark, and ultrasonically for 10min , dilute to the mark with water, shake well, and centrifuge to get ready.

[0072] 1. Selection of chromatographic column

[0073] First, the most commonly used C18 column (WatersAtlantis T3 C 18 ) for pre-testi...

Embodiment 2

[0089] The HPLC-ELSD quantitative detection method of glucosamine and chondroitin sulfate in embodiment 2 health food

[0090] 1. Preparation of mixed reference solution

[0091] Take 5 mg each of glucosamine and chondroitin sulfate reference substances dried at 105°C for 4 hours, accurately weigh them in a 25ml measuring bottle, add 2ml of acetonitrile, oscillate to disperse evenly, then add water, ultrasonically dissolve, cool to room temperature, Dilute water to the mark and shake well to obtain a mixed low-concentration standard solution containing 0.2 mg each of glucosamine and chondroitin sulfate per 1 ml; prepare a mixed low-concentration standard solution containing 0.6 mg each of glucosamine and chondroitin sulfate per 1 ml by the same method solution.

[0092] 2. Preparation of the test solution

[0093] Take this product, remove the film-coated tablet, grind finely, take 100mg of powder, accurately weigh it, place it in a 50mL measuring bottle, add 5mL acetonitril...

Embodiment 3

[0113] The HPLC-ELSD quantitative detection method of glucosamine and chondroitin sulfate in the raw material of embodiment 3

[0114] Take 20 mg of glucosamine sulfate potassium salt or chondroitin sulfate as the raw material, weigh them accurately, place them in a 50 mL measuring bottle, add 5 mL of acetonitrile, disperse by ultrasonic for 1 min, add water to the mark, ultrasonic for 10 min, dilute to the mark with water, shake well, Centrifuge, that is. All the rest are carried out according to Example 2, and the content of the raw material glucosamine sulfate chondroitin potassium salt is 95.37%, 97.80%.

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Abstract

The invention relates to the technical field of analytical chemistry and particularly relates to a method for simultaneously assaying glucosamine and chondroitin sulfate in healthy foods. According tothe method, by adopting an evaporative light scattering detector, the sensitivity of detection on the glucosamine and the chondroitin sulfate is improved; by adopting an amide chromatographic column,the retention time is moderate, the degree of separation is good, the separation of all chromatographic peaks is relatively good, the reference line is stable, the peak form is good, and the repeatability is relatively good. The method has good linear relationship, precision, stability and recovery rate, can be used for accurately assaying the content of the glucosamine and the chondroitin sulfate in the healthy foods simultaneously, is applicable to the assaying of the glucosamine and the chondroitin sulfate in the healthy foods and is also applicable to the assaying of glucosamine or chondroitin sulfate raw materials.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for determining glucosamine and chondroitin sulfate in food or medicine. Background technique [0002] Glucosamine is a hexosamine extracted from natural carapace. It is a natural component of glycoprotein in the joint tissues of humans and animals. It has the functions of anti-inflammatory, pain relief, treatment and repair of articular cartilage tissue. At present, glucosamine and its hydrochloride, sodium sulfate or potassium salt are commonly used in the market. Chondroitin sulfate is an acidic mucopolysaccharide extracted from animal cartilage. It is a biological polymer compound and is mainly used for the treatment of nervous headache, angina pectoris, coronary heart disease, and bone and joint diseases. In recent years, there have been more and more reports on the combined use of chondroitin sulfate, aminodextran, sodium hyaluronate and other drugs i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/60G01N30/74
CPCG01N30/02G01N30/60G01N30/74
Inventor 肖伟于桂芳胡宝玲胡军华曹苗苗林夏王振中
Owner JIANGSU KANION PHARMA CO LTD
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