Preparation method and application of photo-electrochemical immune sensor based on stannic oxide/ stannic sulfide/ mesoporous carbon nitride

An immune sensor and tin disulfide technology, applied in scientific instruments, instruments, and material analysis through electromagnetic means, can solve the problem of reducing the photoelectric conversion efficiency of photoelectric conversion materials, increasing the recombination rate of photoelectric conversion materials, and weakening the light absorption of photoelectric conversion materials and other issues, to achieve good water solubility and biocompatibility, strong photocurrent reduction effect, and good energy band matching.

Active Publication Date: 2018-11-06
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When silicon dioxide / lead sulfide is used as a marker, the insulating property of silicon dioxide will hinder the transfer of electrons; the competitive absorption of light by lead sulfide will weaken the light absorption of photoelectric conversion materials; the consumption of lead sulfide ascorbic acid will increase Recombination rate of photogenerated electron-hole pairs in photoelectric conversion materials
The above three factors synergistically lead to the reduction of photoelectric conversion efficiency of photoelectric conversion materials

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 A preparation method of a photoelectrochemical immunosensor based on tin dioxide / tin disulfide / mesoporous carbon nitride

[0047] 1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 0.1 mol / L sodium hydroxide solution and ultrapure water for 0.5 h, and dried under nitrogen;

[0048] 2) Take 6 µL of tin dioxide / tin disulfide / mesoporous carbon nitride solution and drop it on the conductive surface of ITO conductive glass, and let it dry at room temperature;

[0049] 3) Add 10 mmol / L 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride solution and 20 mmol / L N-hydroxy 4 μL of the mixture of succinimide solution; after incubating at room temperature for 0.5 h, wash the electrode surface with ultrapure water, and let it dry at room temperature to a wet film state;

[0050] 4) Add 6 μL, 1 μg / mL N-terminal pro-BNP precursor capture antibody standard solution dropwise, incubate at 4 °C for 1 h, wash with ultrapure water, and ...

Embodiment 2

[0054] Example 2 A preparation method of a photoelectrochemical immunosensor based on tin dioxide / tin disulfide / mesoporous carbon nitride

[0055] 1. A preparation method based on the photoelectrochemical immunosensor of tin dioxide / tin disulfide / mesoporous carbon nitride, is characterized in that, comprises the following steps:

[0056] 1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 0.1 mol / L sodium hydroxide solution and ultrapure water for 0.5 h, and dried under nitrogen;

[0057] 2) Take 6 µL of tin dioxide / tin disulfide / mesoporous carbon nitride solution and drop it on the conductive surface of ITO conductive glass, and let it dry at room temperature;

[0058] 3) Add 200 mmoL / L 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride solution and 100 mmoL / L N-hydroxy 4 μL of the mixture of succinimide solution; after incubating at room temperature for 0.5 h, wash the electrode surface with ultrapure water, and let it dry at roo...

Embodiment 3

[0063] Example 3 Preparation of tin dioxide / tin disulfide / mesoporous carbon nitride solution

[0064] 1) Preparation and carboxylation of mesoporous carbon nitride

[0065] 3.5 g of urea and 2.9 g of dicyandiamide were blended and ground into powder, placed in a muffle furnace for calcination at 600 °C for 5 h, and cooled to room temperature to obtain mesoporous carbon nitride; 2.0 g of mesoporous carbon nitride Add 100 mL of nitric acid solution with a concentration of 10 mol / L and reflux at 140 °C for 24 h. After cooling to room temperature, the refluxed product is centrifuged and washed 3 times with ultrapure water, and vacuum-dried at 40 °C for 12 h to obtain carboxylated mesoporous nitriding carbon materials;

[0066] 2) Preparation of tin dioxide / tin disulfide / mesoporous carbon nitride

[0067] Dissolve 0.1 g of carboxylated mesoporous carbon nitride in 10–50 mL of ultrapure water. After ultrasonication for 1 h, 1.38 g of tin tetrachloride pentahydrate was added to the...

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Abstract

The invention relates to a preparation method and application of a photo-electrochemical immune sensor based on stannic oxide / stannic sulfide / mesoporous carbon nitride. According to the method, stannic oxide / stannic sulfide / mesoporous carbon nitride are taken as a substrate material, and visible light is used for illumination to obtain light current; three components of the substrate materialcan be matched well, so that the photovoltaic conversion efficiency is greatly improved; silicon dioxide / lead sulfide used as second antibody markers have obvious quenching action on the light current; the quantity difference of to-be-tested amino-terminal pro-brain natriuretic peptide causes the quantity difference of combined second antibodies and second antibody markers, thereby causing the difference of influence degree on optoelectronic signals; the constructed sensor achieves the detection of the amino-terminal pro-brain natriuretic peptide, and the detection limit is 50 fg / mL.

Description

technical field [0001] The invention relates to the preparation method and application of a photoelectrochemical immunosensor based on tin dioxide / tin disulfide / mesoporous carbon nitride, specifically adopting tin dioxide / tin disulfide / mesoporous carbon nitride as the base material, Silicon / lead sulfide is used as a secondary antibody marker to prepare a photoelectrochemical immunosensor for sensitive detection of amino-terminal brain natriuretic peptide precursor, which belongs to the field of new functional materials and biosensing detection technology. Background technique [0002] The secretion and existence of N-terminal pro-BNP in the blood has an accumulation effect, and it is stable in vitro for a long time. It has good detection stability and high detection sensitivity. An important indicator of prognosis and treatment monitoring. [0003] At present, there are many clinical detection methods for N-terminal pro-BNP. The patent (authorized notification number CN204...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/327
CPCG01N27/3278G01N33/68
Inventor 魏琴张益峰冯锐魏东杜斌张勇孙旭杨兴龙
Owner UNIV OF JINAN
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